The largest database of trusted experimental protocols

3 protocols using caspase 12

1

Histopathological Analysis of Liver and Pancreas

Check if the same lab product or an alternative is used in the 5 most similar protocols
After euthanasia, the liver and pancreas were processed, including 10% neutral formaldehyde fixing, paraffin embedding, 5 µm thickness sectioning, and H&E staining [26 (link)]. The pathological changes were observed using a stereomicroscope (SMZ25, Nikon, Tokyo, Japan). For immunohistochemistry, the sections of the liver were antigen retrieval, blocked with goat serum (37 °C for 20 min), and then incubated with primary antibodies. The antibodies included AMPK (1:100, Beyotime, Shanghai, China), Phosphorylated protein kinase RNA-like endoplasmic reticulum kinase (p-PERK, 1:200, Bioss, Beijing, China), Dynamic-related protein 1 (DRP1, 1:100, Beyotime), Cytochrome c (CytC, 1:100, Proteintech, Chicago, IL, USA), caspase 12 (1:100, Bioss), Inositol-requiring enzyme-1 (IRE1, 1:100, Proteintech) and Mitofusion 1/2 (MFN1/2, 1:100, Proteintech). Subsequently, the slides were visualized using DAB and then observed under a stereomicroscope (SMZ25, Nikon).
+ Open protocol
+ Expand
2

Western Blot Analysis of ER Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was abstracted from frozen intestinal tissue. Extracted protein was separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to nitrocellulose blotting membranes (Roche, CA, USA). The membranes were incubated with primary antibodies to Tubulin (Proteintech, wuhan, china), Grp78 (Bioworld, USA), Caspase-12 (Bioss, BeiJing, China), Gadd153 (Bioworld, USA), ATF4 (Santa Cruz, USA), P-elF2 (Bioeasy, shanghai, china) and P-PERK (Santa Cruz, USA), and horseradish-peroxidase–conjugated secondary antibodies for 2 hours, then developed with SuperSignal West Pico chemiluminescent Substrate Kit (Pierce, Rockford, USA), and finally analyzed with the Bio-Image Analysis System (Syngene, Frederick, MD, USA).
+ Open protocol
+ Expand
3

Protective Effects of HDAX on ER Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
HDAX were mainly composed of Coptidis Rhizoma, Radix Rehmanniae, Radix Puerariae, Radix Ophiopogonis, Eriobotryae Folium, and Radix Notoginseng, which were purchased from Anhui Provincial Hospital of Chinese Medicine. Notoginsenoside R1, ginsenoside Rg1, puerarin, acteoside, berberine, coptisine, and palmatine hydrochloride were purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). 4-phenylbutyric acid (4-PBA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibody of GRP78, CHOP was purchased from A nity Bioscience (1:1300, USA); Caspase-12, Caspase-9, Caspase-3 were obtained from Bioss (1:1000, Beijing, China); Bax, Bcl-2 was provided by Bioworld (1:1000, Nanjing, China).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!