Microplate reader

Cells were seeded in 96-well plates. A2780 and A2780cis cells were seeded with 1 × 10⁴ cells per well and other cell lines were seeded with 9 × 10³ cells per well. Cells were incubated in 37 °C for 24 h. WOD, MOD, UA, OA, and cisplatin were added in various concentrations, and incubation continued for 48 h. After treatment, 15 μL 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) (Sigma Aldrich, St, Louis MO, USA) stock solution in PBS (5 mg/mL) was added to each well. After 4 h at 37 °C, the medium was removed, and cells were solubilized in 100 μL dimethyl sulphoxide (DMSO). The viability of cells was read using a microplate reader (Biochrome, Berlin, Germany) at 562 nm.

NK-92MI cells were treated with 25KbPEI for 12 h, after which time 1 × 10⁶ cells were co-incubated (for 4 h at 37 °C/5% CO₂) with target A2780, OVCAR3, SKOV3, and MDA-MB231 cancer cells at an effector:target (E:T) ratio of 10:1. Then, the cell culture supernatants were harvested to measure secreted IFN-γ using the Human IFN-γ ELISA Set (555142, BD Biosciences, San Jose, CA). Absorbance was measured in a microplate reader (Biochrome, Berlin, Germany) at a wavelength of 450 nm.