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Nb300 141

Manufactured by Cell Signaling Technology
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NB300-141 is a primary antibody that recognizes the phosphorylated form of p70 S6 kinase (Thr389). It can be used for Western blotting, immunoprecipitation, and immunohistochemistry applications.

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Lab products found in correlation

Anti neun, by Cell Signaling Technology (2 mentions) H00009572 mo2, by Abnova (2 mentions) Lsm 880 microscope, by Zeiss (2 mentions)

2 protocols using nb300 141

1

Quantifying NR1D1-Positive Nuclei in Mouse Spinal Cord

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Antigen retrieval and staining in paraffin‐embedded tissues was performed as previously described.35 Mice lumbar spinal cord sections were stained with anti‐GFAP (Novus, NB300‐141, lot 179‐071616) or anti‐NeuN (Cell Signaling, 24307, lot 2) and anti‐NR1D1 (Abnova, H00009572‐MO2, lot J9051‐4F6) antibodies. Nuclei were counterstained with DAPI (4′,6‐diamidino‐2‐phenylindole dihydrochloride). Images were acquired on an LSM 880 microscope (Zeiss) with Zen Black (v2.3) image software. To determine the percent of NR1D1‐positive nuclei, images were analyzed using ImageJ (version 1.52p). After setting a threshold, individual image channels were converted to binary, overlaid, and then the number of nuclei containing overlapping areas were obtained using the Analyze Particles function. In each group, there were four or five mice and two to four images were analyzed per animal.
Killoy K.M., Harlan B.A., Pehar M, & Vargas M.R. (2022). NR1D1 downregulation in astrocytes induces a phenotype that is detrimental to cocultured motor neurons. The FASEB Journal, 36(4), e22262.
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2

NR1D1 Expression in Spinal Cord Cells

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Antigen retrieval and staining in paraffin-embedded tissues was performed as previously described (35 ). Mice lumbar spinal cord sections were stained with anti-GFAP (Novus, NB300–141, lot 179–071616) or anti-NeuN (Cell Signaling, 24307, lot 2) and anti-NR1D1 (Abnova, H00009572-MO2, lot J9051–4F6) antibodies. Nuclei were counterstained with DAPI (4’,6-Diamidino-2-phenylindole dihydrochloride). Images were acquired on an LSM 880 microscope (Zeiss) with Zen Black (v2.3) image software. To determine the percent of NR1D1-positive nuclei, images were analyzed using ImageJ (version 1.52p). After setting a threshold, individual image channels were converted to binary, overlaid, and then the number of nuclei containing overlapping areas were obtained using the Analyze Particles function. In each group, there were 4–5 mice and 2–4 images were analyzed per animal.
Killoy K.M., Harlan B.A., Pehar M, & Vargas M.R. (2022). NR1D1 downregulation in astrocytes induces a phenotype that is detrimental to cocultured motor neurons. The FASEB Journal, 36(4), e22262.
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