Quantification of confocal images of melanoma cell motility, reporter and pigment intensity in LSM Zen 2009 (Carl Zeiss) and pigment/EZH2 intensity in human tissue array performed in ImageJ. Cellular pigment or reporter intensity was corrected for background levels and then normalised to the average intensity for the whole image. Cell area measured in Volocity (Perkin Elmer). Cell tracking was performed with ImageJ Manual Tracking Plug-in. Overlapping tracks of motile cells were defined as parallel tracks within 5μm of each other laterally, with overlap of longer than 12μm and cells present within 15 minutes of each other. Persistence was calculated by actual distance travelled by the cell divided by the Euclidean distance.
Lsm zen 2009
Manufactured by Zeiss
Sourced in Germany
The LSM Zen 2009 is a laser scanning microscope system developed by Zeiss. It provides high-resolution imaging capabilities for various scientific and research applications. The system combines advanced optical components, precise scanning mechanisms, and a modular design to enable flexible configuration and customization to meet user requirements.
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3 protocols using lsm zen 2009
1
Melanoma Cell Motility and Pigment Quantification
2
Melanoma Cell Motility and Pigment Quantification
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Quantification of confocal images of melanoma cell motility, reporter and pigment intensity in LSM Zen 2009 (Carl Zeiss) and pigment/EZH2 intensity in human tissue array performed in ImageJ. Cellular pigment or reporter intensity was corrected for background levels and then normalised to the average intensity for the whole image. Cell area measured in Volocity (Perkin Elmer). Cell tracking was performed with ImageJ Manual Tracking Plug-in. Overlapping tracks of motile cells were defined as parallel tracks within 5μm of each other laterally, with overlap of longer than 12μm and cells present within 15 minutes of each other. Persistence was calculated by actual distance travelled by the cell divided by the Euclidean distance.
3
Prolong Diamond Mounting for Microscopy
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Samples were mounted with ProLong Diamond (Thermo Fisher Scientific). Images were obtained using a LSM5 Pascal or LSM710 microscope and analyzed using LSM5 Pascal Image and LSM ZEN2009 software (Carl Zeiss, Oberkochen, Germany).
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