Caspase 3 (AC-DEVD-AMC) and Caspase 9 (AC-LEHD-AMC) substrates were obtained from Enzo (Lausen, Switzerland). APOPercentage assays with releasing buffer were obtained from Biocolor (Belfast, Northern Ireland). A mitochondrial stain 5,50, 6,60-tetrachloro-1,10,3,30-tetraethylbenzimidazolyl carbocyanine iodide (JC-1) and Probenecid were obtained from Santa Cruz (Dallas, Texas, USA). Pluronic® F-127 was obtained from Biovision(San Francisco, USA). Dihydrorhodamine-123 (DHR 123), was obtained from Sigma Aldrich (St. Louis, MO), Fura-2 AM calcium fl orescent dye was obtained from Calbiochem (Darmstadt, Germany).
Caspase 3 ac devd amc
Manufactured by Enzo Life Sciences
Sourced in Switzerland, United States
Caspase 3 (AC-DEVD-AMC) is a fluorogenic substrate used for the detection and measurement of caspase-3 activity. Caspase-3 is a key executioner caspase involved in the apoptosis (programmed cell death) pathway. The AC-DEVD-AMC substrate contains the caspase-3 cleavage sequence DEVD, which, upon cleavage by active caspase-3, releases the fluorescent AMC (7-amino-4-methylcoumarin) moiety, allowing for quantitative measurement of caspase-3 activity.
Automatically generated - may contain errors
Lab products found in correlation
Dihydrorhodamine 123 dhr123, by Merck Group (1 mentions)
Pluronic f 127, by Abcam (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Wst 1 assay, by Roche (1 mentions)
2 protocols using caspase 3 ac devd amc
1
Apoptosis and Mitochondrial Assays
2
Isoquercitrin Extraction and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organic solvents of analytical grade were purchased either from POCh S.A. (Gliwice, Poland) or from Merck (Darmstadt, Germany). Water was purified by a Milli-Q system (Millipore Corp., Bedford, MA, USA). MeOH and MeCN of HPLC grade were purchased from Merck (Darmstadt, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were from Gibco (Invitrogen, Paisley, UK). WST-1 assay was purchased from Roche Diagnostic (Basel, Switzerland). Caspase-3 (Ac-DEVD-AMC) and cathepsin D (MOCA-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2) fluorogenic substrates were obtained from Enzo Life Sciences (New York, NY, USA). All other reagents were from Sigma (Sigma-Aldrich Chemie GmbH, Schnelldorf, Germany). Isoquercitrin (purity > 95% by HPLC) was isolated from flowers of Xerolekia speciosissima (L.) Anderb. [60 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!