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Ventanabenchmark xt automatic

Manufactured by Roche
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The VentanaBenchmark XT is an automated immunohistochemistry (IHC) and in situ hybridization (ISH) staining system. The core function of the VentanaBenchmark XT is to automate the staining process, allowing for consistent and reproducible results in a clinical or research laboratory setting.

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Lab products found in correlation

Ultraview universal dab detection kit, by Roche (2 mentions) Desmin, by Agilent Technologies (1 mentions) Anti ki67, by Thermo Fisher Scientific (1 mentions) Ae1 ae3, by Agilent Technologies (1 mentions) Bcor c 10, by Santa Cruz Biotechnology (1 mentions) Myogenin f5d, by Agilent Technologies (1 mentions) Cd34 qbend 10, by Agilent Technologies (1 mentions) Desmin d33, by Agilent Technologies (1 mentions) Ema e29, by Agilent Technologies (1 mentions) Ini 1, by BD (1 mentions)

2 protocols using ventanabenchmark xt automatic

1

Histological Analysis of FFPE Tissues

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Histological examination was performed on 4 µm-thick sections cut from formalin-fixedparaffin-embedded (FFPE) tissue and stained with hematoxylin and eosin. The following primary antibodies were used: anti-Ki-67 (1/100; rabbit monoclonal, clone SP6, Thermo Fischer Scientific USA), Desmin (1/80; mouse monoclonal, clone D33, Agilent, Courtaboeuf, France), Myogenin (1/1; mouse monoclonal, clone F5D, Agilent), Myod1 (1/1, mouse EP212, Roche diagnostics). Immunostaining was performed on a VentanaBenchmark XT automatic stainer (Ventana, Tuscon, AZ) and revealed with the ultraview Universal Dab Detection kit.
Karanian M., Pissaloux D., Gomez-Brouchet A., Chevenet C., Le Loarer F., Fernandez C., Minard V., Corradini N., Castex M.P., Duc-Gallet A., Blay J.Y, & Tirode F. (2020). SRF-FOXO1 and SRF-NCOA1 Fusion Genes Delineate a Distinctive Subset of Well-differentiated Rhabdomyosarcoma. The American journal of surgical pathology, 44(5).
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2

Immunohistochemical Analysis of Soft Tissue Tumors

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Sections were cut at 4 μm thick from formalin-fixed-paraffin-embedded (FFPE) tissue and immunostained using a VentanaBenchmark XT automatic stainer (Ventana, Tuscon, AZ, USA). Signals were revealed using the ultraView Universal Dab Detection kit (Ventana). The following antibodies were used: CD99 (12E7, Dako), EMA (E29, 1:50, Dako), desmin (D33, 1:80, Dako), cytokeratin AE1/AE3 (AE1/AE3, 1:50, Dako), caldesmon (h-CD, 1:100, Dako), myogenin (F5D, 1:100, Dako), S100 protein (Z0311, 1:800, Dako), CD34 (QBend-10, 1:25, Dako), INI1 (25, 1:50, BD Transduction Laboratories), BCOR (C-10, 1:50, Santa Cruz), ETV4 (16, 1:50, Santa Cruz). Immunohistochemistry for NTRK1 was performed using a 32 min incubation with the NTRK1 antibody (ab76291, clot EP1058Y, Abcam, dilution 1:200) on a Ventana ULTRA machine with Cell Conditioning Solution 1 pre-treatment for 64 min.
Alholle A., Karanian M., Brini A.T., Morris M.R., Kannappan V., Niada S., Niblett A., Ranchère-Vince D., Pissaloux D., Delfour C., Gonzalez A.M., Antonescu C.R., Sumathi V., Tirode F, & Latif F. (2018). Genetic analyses of undifferentiated small round cell sarcoma identifies a novel sarcoma subtype with a recurrent CRTC1-SS18 gene fusion. The Journal of pathology, 245(2), 186-196.
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