Standard western blotting was conducted for protein expression assays in hypoxic H9C2 cells transfected with miR-210 mimic, inhibitor, and NC. Two days following transfection, proteins were isolated with RIPA lysis buffer containing 1 mg protease inhibitors (Applygen Technologies, Beijing, China). The protein contents were quantified using the Bicinchoninic Acid (BCA) Protein Assay Kit (CoWin Biotech, Beijing, China). Primary antibodies against light chain 3 (LC3; ab48394), p62 (ab56416), Caspase8ap2 (ab4052), cleaved caspase 8 (ab25901), cleaved caspase 3 (ab13847), Beclin-1 (ab62557), and the internal controls β-actin and GAPDH were from Abcam (Cambridge, UK). Furthermore, secondary antibodies labelled with horseradish peroxidase were incubated with membranes for 1 h at 37°C. Samples were then transferred to a polyvinylidene fluoride (PVDF) microporous membrane (Millipore, Boston, MA, USA). Blots were imaged by a FluorChem E imager (Cell Biosciences, San Jose, CA, USA). The grey value of each band was determined with ImageJ version 1.51.
Ab4052
Manufactured by Abcam
Sourced in United Kingdom
Ab4052 is a laboratory equipment product manufactured by Abcam. It is designed for use in scientific research and analysis. The core function of this product is to provide a specific capability or tool for researchers to utilize in their experiments and studies.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by CoWin Biotech (2 mentions)
Pvdf microporous membrane, by Merck Group (2 mentions)
Protease inhibitor, by Applygen (2 mentions)
Fluorcheme imager, by Cell Biosciences (2 mentions)
Ab13847, by Abcam (2 mentions)
Ab62557, by Abcam (2 mentions)
Ab56416, by Abcam (2 mentions)
Ab25901, by Abcam (2 mentions)
β actin, by Abcam (2 mentions)
Ripa lysis buffer, by Applygen (2 mentions)
Gapdh, by Abcam (1 mentions)
Ab48394, by Abcam (1 mentions)
2 protocols using ab4052
1
Protein Expression Profiling of Hypoxic H9C2 Cells
2
Hypoxic H9C2 Cell Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard western blotting was conducted for protein expression assays in hypoxic H9C2 cells transfected with miR-210 mimic, inhibitor and NC. Two days following transfection, proteins were isolated with RIPA lysis buffer containing 1 mg protease inhibitors (Applygen Technologies Inc., Beijing, China). The protein contents were quantified using the Bicinchoninic Acid (BCA) Protein Assay Kit (CoWin Biotech Co., Ltd., Beijing, China). Primary antibodies against light chain 3 (LC3; ab48394), p62 (ab56416), Caspase8ap2 (ab4052), cleaved caspase 8 (ab25901), cleaved caspase 3 (ab13847), Beclin-1 (ab62557), and the internal controls β-actin and GAPDH were from Abcam (Cambridge, UK). Furthermore, secondary antibodies labelled with horseradish peroxidase were incubated with membranes for 1 h at 37°C. Samples were then transferred to a polyvinylidene fluoride (PVDF) microporous membrane (Millipore, Boston, USA). Blots were imaged by a FluorChem E imager (Cell Biosciences, San Jose, USA). The grey value of each band was determined with ImageJ version 1.51.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!