Tecnai t12 cryo electron microscope

5 µl α-syn at 10 µM concentration was spotted on freshly glow-discharged Formvar-Carbon film 400 mesh copper grids (Electron Microscopy Sciences). After 4 min incubation, grids were rinsed with 5 μL ultrapure water and stained with 3 μl filter sterilized uranyl acetate solution (2% w/v in ultrapure water) for 4 min. Excess uranyl acetate was removed by blotting and another 3 μl of 2% uranyl acetate was spotted on the grid. After 3 min incubation, excess uranyl acetate was removed by blotting and the grids were allowed to air dry for 5 min. TEM images were taken using a FEI Tecnai T12 cryo-electron microscope at 120 kV with 15,000X magnification.

Transmission electron microscopy (TEM) images were acquired on Tecnai T12 Cryo–electron microscope (FEI) operating with an acceleration voltage of 120 kV. Dynamic light scattering (DLS) measurements were performed on a Zetasizer Nano instrument (Malvern) with a 10-mW helium-neon laser and thermoelectric temperature controller. TMS was used as internal standard, and 1H nuclear magnetic resonance (NMR) spectra were recorded on a 500-MHz Bruker AV400 spectrometer. FTIR measurements were performed using an FTIR spectrometer (Nicolet 6700, Thermo). X-ray photoelectron spectroscopy measurements were performed using an x-ray photoelectron spectroscopy (ESCALAB 250Thermo). Fluorescence intensity imaging was performed using an Automatic multi-function imaging analysis system (OI600-MF-Touch). Cell adhesion on the surface of the material was observed by SEM (CarlZeiss SMT, Germany) imaging. Fluorescence intensity was measured with microplate reader Multiskan GO (Thermo). Fluorescently stained cells were imaged with an OLYMPUS upright fluorescence microscope (Japan). UV absorption measurement was used by NANODROP (Thermo).