Cells were collected using a scraper, washed with PBS and resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 mM EGTA) containing protease and phosphatase inhibitor cocktail (Roche Molecular Biochemicals). The following antibodies with corresponding concentrations were used: anti-PAR (Trevigen, 1:1000), anti-Chk2 (EMD Millipore, 1:1000), anti-pChk2-T68 (Cell Signaling, 1:1000), anti-Chk1 (Santa Cruz, 1:750), anti-pChk1-S345 (Cell Signaling, 1:1000), anti-pKAP1-S824 (Bethyl Laboratories, 1:2000), anti-Cas9 (Cell Signaling Technology, 1:1000), anti-Clathrin Heavy Chain (BD Transduction Laboratories, 1:10 000) and anti-Tubulin (Sigma, 1:10 000).
Anti pkap1 s824
Manufactured by Fortis Life Sciences
Anti-pKAP1-S824 is a rabbit polyclonal antibody that detects phosphorylated KRAB-associated protein 1 (KAPA1) at serine 824. It is designed for use in Western blotting and immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti chk1, by Santa Cruz Biotechnology (2 mentions)
Anti pchk1 s345, by Cell Signaling Technology (2 mentions)
Anti clathrin heavy chain, by BD (1 mentions)
Anti cas9, by Cell Signaling Technology (1 mentions)
Anti par, by Bio-Techne (1 mentions)
Anti pchk2 t68, by Cell Signaling Technology (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Protease and phosphatase inhibitor cocktail, by Roche (1 mentions)
Anti brca1, by Cell Signaling Technology (1 mentions)
Anti v5, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Thermo Fisher Scientific (1 mentions)
Donkey anti rabbit igg hrp, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg hrp, by Thermo Fisher Scientific (1 mentions)
Anti patm s1981, by Cell Signaling Technology (1 mentions)
Anti blm, by Fortis Life Sciences (1 mentions)
Anti dna pkcs, by Fortis Life Sciences (1 mentions)
Anti kap1, by Fortis Life Sciences (1 mentions)
Anti ctip, by Fortis Life Sciences (1 mentions)
Anti rad51, by Merck Group (1 mentions)
2 protocols using anti pkap1 s824
1
Chk1/Chk2 Signaling Pathway Analysis
2
DNA Damage Response Pathway Profiling
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The following primary antibodies were used: anti-β-Actin (Thermo Fisher Scientific; #MA1-140), anti-pDNA-PKcs-S2056 (Thermo Fisher Scientific; #PA5-78130), anti-DNA-PKcs (Bethyl Laboratories; #A300-516A), anti-pRPA2-S4/8 (Bethyl Laboratories; #A300-245A), anti-RAD51 (Merck Millipore; #PC130), anti-pKAP1-S824 (Bethyl Laboratories; #A300-767A), anti-pATM-S1981 (Cell Signaling Technology; #13050S), anti-KAP1 (Bethyl Laboratories; #A300-274A), anti-pCHK1-S345 (Cell Signaling Technology; #2341), anti-CHK1 (Santa Cruz Biotechnology; #sc-8408), anti-pH2AX-S139 (Bethyl Laboratories; A300-081A), anti-BRCA1 (Cell Signaling Technology; #9010), anti-CTIP (Bethyl Laboratories; #A300-488A), anti-BLM (Bethyl Laboratories; #A300-110A), anti-V5 (Thermo Fisher Scientific; #R960-25). The secondary antibodies used were Goat anti-Mouse IgG-HRP (Thermo Fisher Scientific; #NA931V) and Donkey anti-Rabbit IgG-HRP (Thermo Fisher Scientific; #NA934V).
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