MyoVin-1 (Millipore), Pentabromopseudalin (PBP, Fisher Scientific) or EGTA-AM (Millipore) were diluted in DMSO (Sigma-Aldrich) and stored at −20°C. Samples were incubated in imaging solution with 30 μM Myo-1 for 5–10 min or 5 μM PBP for 5 min, or 250 μM EGTA-AM for 20 min before dye loading. The effective final DMSO concentration was < 0.5%. Extended exposure to MyoVin-1 or PBP caused cell death, thus the bath solution during the experiment did not include Myo-1 or PBP. Our control measurements indicated that continuous presence of these blockers during the experiments did not have additional effects on vesicle motility beyond the effects of pre-incubation (data not shown).
Myovin 1
Manufactured by Merck Group
Sourced in United States
MyoVin-1 is a lab equipment product manufactured by Merck Group. It is a small molecule that acts as a selective inhibitor of myosin II ATPase activity. The core function of MyoVin-1 is to provide researchers with a tool for studying the role of myosin II in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Egta am, by Merck Group (3 mentions)
Latrunculin a, by Merck Group (2 mentions)
Ouabain, by Merck Group (1 mentions)
Bapta am, by Merck Group (1 mentions)
Bapta, by Merck Group (1 mentions)
Amiloride eipa, by Merck Group (1 mentions)
Gadolinium 3 chloride, by Merck Group (1 mentions)
Ck 666, by Merck Group (1 mentions)
Cytochalasin d, by Merck Group (1 mentions)
Para amino blebbistatin, by Cayman Chemical (1 mentions)
5 protocols using myovin 1
1
Vesicle Motility Modulation Assay
2
Inhibition of Myosin-Based Vesicle Motility
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MyoVin-1 (Millipore), Pentabromopseudalin (PBP, Fisher Scientific) or EGTA-AM (Millipore) were diluted in DMSO (Sigma-Aldrich) and stored at −20°C. Samples were incubated in imaging solution with 30 µM Myo-1 for 5–10 min or 5 µM PBP for 5 min, or 250 µM EGTA-AM for 20 min before dye loading. The effective final DMSO concentration was <0.5%. Extended exposure to MyoVin-1 or PBP caused cell death, thus the bath solution during the experiment did not include Myo-1 or PBP. Our control measurements indicated that continuous presence of these blockers during the experiments did not have additional effects on vesicle motility beyond the effects of pre-incubation (data not shown).
3
Vesicle Motility Inhibition Protocols
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MyoVin-1 (Millipore), Pentabromopseudalin (PBP, Fisher Scientific) or EGTA-AM (Millipore) were diluted in DMSO (Sigma-Aldrich) and stored at -20°C. Samples were incubated in imaging solution with 30 µM Myo-1 for 5-10 min or 5 µM PBP for 5 min, or 250 µM EGTA-AM for 20 min before dye loading. The effective final DMSO concentration was <0.5%. Extended exposure to MyoVin-1 or PBP caused cell death, thus the bath solution during the experiment did not include Myo-1 or PBP. Our control measurements indicated that continuous presence of these blockers during the experiments did not have additional effects on vesicle motility beyond the effects of pre-incubation (data not shown).
4
Inhibition Assay for Cell Motility
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Latrunculin A (Sigma–Aldrich), 5‐(N‐Ethyl‐N‐isopropyl) amiloride (EIPA, Sigma–Aldrich), Ouabain (Sigma–Aldrich), Bumetanide (Ro 10–6338, Santa Cruz Biotechnology), Rab7 inhibitior CID 1067700 (Sigma–Aldrich), MyoVin‐1 (Sigma–Aldrich), BAPTA (Sigma–Aldrich), BAPTA‐AM (Sigma–Aldrich), FTY720, 2‐APB and CAI (Tocris Biosciences), Calpain inhibitor I (Millipore Sigma) in dimethylsulfoxide and Gadolinium (III) Chloride (Sigma–Aldrich) in water were used. For all inhibition experiments, cells were incubated in cell culture media with drugs for a duration of 1 h (except Ouabain ≈2 h) and then, high viscosity media was added. Cells were then imaged over a span of 5 h for speed measurements or were incubated over a span of ≈4 h for immunostaining.
5
Actin and Myosin Inhibitor Concentrations
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The following reagents and working concentrations were used for actin and myosin inhibitors: cytochalasin D (0.5 μM and 1 μM, C8273), latrunculin A (0.5 μM and 2.5 μM, L5163), CK-666 (25 μM and 50 μM, SML0006) and myoVin1 (0.5 μM and 1.5 μM, 475984), were obtain from Sigma, USA. para-aminoblebbistatin (5 μM and 20 μM, 22699) was purchased from Cayman Chemical, USA.
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