Human CRC HCT-8 cells and 5-FU resistant HCT-8/5-FU cells were obtained from Nanjing KeyGen Biotech. Co., Ltd. Cells were grown in RPMI-1640 medium, supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. In addition, HCT-8/5-FU cell cultures were supplemented with 15 µg/ml 5-FU. Cells were cultured at 37°C and 5% CO2 in a humidified incubator. The cells were subcultured at a ratio of 1:4-1:6 at between 80 and 90% confluency.
Hct 8 cells
Manufactured by Keygen Biotech
Sourced in China
The HCT-8 cells are a well-established human colorectal adenocarcinoma cell line. They are commonly used in cancer research and drug development. The HCT-8 cells exhibit epithelial-like morphology and have a doubling time of approximately 20-24 hours.
Automatically generated - may contain errors
2 protocols using hct 8 cells
1
CRC Cell Line Maintenance Protocol
2
Cell Culture Protocols for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HCT116 cells, RKO cells, SW480 cells, HCT8 cells, HT29 cells, LOVO cells, and FHC cells were obtained from the KeyGEN BioTECH (Nanjing, Jiangsu, China). Cell culture was performed in an atmosphere of 5% CO2 at 37°C.
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