Xn 9000 analyzer

Manufactured by Sysmex

Sourced in Japan, Germany

The XN-9000 analyzer is a hematology instrument developed by Sysmex. It is designed to perform complete blood count (CBC) and other related tests. The XN-9000 utilizes advanced fluorescence flow cytometry technology to provide accurate and reliable results for a wide range of hematological parameters.

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Lab products found in correlation

Vacuette tube, by Greiner (3 mentions) Au5800, by Beckman Coulter (3 mentions) Cobas 8000, by Roche (2 mentions) Dimension vista analyzer, by Siemens (1 mentions) Dy206, by R&D Systems (1 mentions) G8 analyzer, by Tosoh (1 mentions) Cobas 8000 modular analyzer, by Roche (1 mentions) Enzyme linked immunosorbent assay, by DRG International (1 mentions) Dxi800, by Beckman Coulter (1 mentions) Coulter access 2, by Beckman Coulter (1 mentions) Cobas e411, by Roche (1 mentions) Histopaque, by Merck Group (1 mentions) Cobas 8000 system, by Roche (1 mentions) Au5800 analyzer, by Beckman Coulter (1 mentions) Luminex multiplex assay, by R&D Systems (1 mentions) Bio plex 200, by Bio-Rad (1 mentions) Biophen heparin lrt, by Hyphen Biomed (1 mentions) Dade actin fsl, by Siemens (1 mentions) Dade innovin, by Siemens (1 mentions) Elecsys ferritin, by Roche (1 mentions)

Spelling variants of this product

XN-9000 (290 citations) XN-9000 hematology analyzer (31 citations) Sysmex XN-9000 Automated Hematology Analyzer (2 citations) XN-9000 automatic hematology analyzer (2 citations)

25 protocols using xn 9000 analyzer

Blood Sampling and Platelet Isolation

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Blood was obtained by venipuncture from healthy volunteers, who had not received anti-platelet medication for at least two weeks and provided full informed consent according to the declaration of Helsinki. The study was approved by the Medical Ethics Committee of Maastricht University Medical Centre⁺ (NL31480.068.10). Blood was collected into 3.2% trisodium citrate after discarding the first 3 ml of blood (Vacuette tubes, Greiner Bio-One, Alphen a/d Rijn, The Netherlands).
For the small molecule screening, two bags of 330 mL platelet concentrates were obtained from Stichting Sanquin (Amsterdam, The Netherlands; permission NVT0505.01). These concentrates were pooled from five healthy donors with identical ABO and Rh(D) compatible blood types (11 × 10⁹ platelets/mL PAS-E plasma). The platelet batches were available after safety screening, at two days after blood drawing. Platelets were isolated immediately after receival and checked for full responsiveness to CRP and thrombin. Platelet count and hematological parameters were measured with an XN-9000 analyzer (Sysmex, Kobe, Japan).

Fernández D.I., Troitiño S., Sobota V., Tullemans B.M., Zou J., van den Hurk H., García Á., Honarnejad S., Kuijpers M.J, & Heemskerk J.W. (2024). Ultra-high throughput-based screening for the discovery of antiplatelet drugs affecting receptor dependent calcium signaling dynamics. Scientific Reports, 14, 6229.

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Diabetes Impact on Carotid Plaque Characteristics

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Demographics, vascular risk factors (hypertension, active smoking, type 2 diabetes, dyslipidemia with detailed treatment), chronic renal insufficiency and symptomatic stenosis were collected. Patients were divided into groups according to their diabetic status: control group and diabetic group. Preoperative plaque imaging assessment included for all patients doppler ultrasound evaluating plaque echolucency and degree of stenosis according to NASCET measures, and CT angiographies (CTA) describing the presence of plaque ulceration and calcification. Glycated hemoglobin A1c (HbA1c) was measured by high pressure liquid chromatography on a Tosoh G8 analyzer (Tosoh Bioscience, Tokyo, Japan), C reactive protein (CRP), troponin I, creatinine, total cholesterol, HDL cholesterol and triglycerides were determined on a Dimension Vista analyzer using dedicated reagents (Siemens). Perioperative hematology parameters were determined on a XN-9000 analyzer (Sysmex). Serum IL-1β levels were determined using a high-sensitivity enzyme-linked immunosorbent assay (ThermoFisher Scientific) with a limit of quantification (LOQ) of 0.05 pg/mL. IL-6 and IL-18 were determined by commercial ELISA Kit (Human IL-6 and Human Total IL-18 DuoSet ELISA Ref DY318 and DY206, R&D System, Minneapolis, MN, USA).

Ménégaut L., Laubriet A., Crespy V., Leleu D., Pilot T., Van Dongen K., de Barros J.P., Gautier T., Petit J.M., Thomas C., Nguyen M., Steinmetz E, & Masson D. (2023). Inflammation and oxidative stress markers in type 2 diabetes patients with Advanced Carotid atherosclerosis. Cardiovascular Diabetology, 22, 248.

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Early Cerebral Infarction Cortisol Dynamics

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Peripheral blood from patients with early cerebral infarction (day 3) was collected in Goyang, South Korea. Patients with stroke were 68.8 ± 3.2 years old (mean ± SEM, n = 20, 65% male). Blood samples from patients diagnosed with dizziness, headaches, and weaknesses were used as controls. The specimens were collected over a 4-month period in a non-consecutive cohort. Sampling for cortisol levels was performed between 7:30 and 8:00 AM. Control patients were 61 ± 2.5 years old (mean ± SEM, n = 14, 50% male). Hematological measurements (Complete Blood Count) were obtained using the XN-9000 analyzer (Sysmex) at the Department of Laboratory Medicine, Dongguk University Ilsan Hospital, Goyang, South Korea. Serum cortisol concentration was measured using a competitive immunoassay (Elecsys cortisol assay) in a Cobas® 8000 Modular Analyzer (Roche Diagnostics). The Institutional Review Board of Dongguk University Ilsan Hospital approved the study. All patients or their legally authorized representatives gave a written informed consent.

Courties G., Frodermann V., Honold L., Zheng Y., Herisson F., Schloss M.J., Sun Y., Presumey J., Severe N., Engblom C., Hulsmans M., Cremer S., Rohde D., Pittet M.J., Scadden D.T., Swirski F.K., Kim D.E., Moskowitz M.A, & Nahrendorf M. (2019). Glucocorticoids Regulate Bone Marrow B Lymphopoiesis After Stroke. Circulation research, 124(9), 1372-1385.

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Healthy Volunteer Blood Collection

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Blood was obtained through venipuncture from healthy volunteers who had not received antiplatelet medication for at least two weeks. All subjects gave their full informed consent according to the Declaration of Helsinki. Studies were approved by the local Medical Ethics Committee. Blood samples were collected into 3.2% trisodium citrate (Vacuette tubes, Greiner Bio-One, Alphen a/d Rijn, the Netherlands). All subjects had platelet counts within the reference range, as measured with a Sysmex XN-9000 analyzer (Sysmex, Cho-ku, Kobe, Japan).

Jooss N.J., De Simone I., Provenzale I., Fernández D.I., Brouns S.L., Farndale R.W., Henskens Y.M., Kuijpers M.J., ten Cate H., van der Meijden P.E., Cavill R, & Heemskerk J.W. (2019). Role of Platelet Glycoprotein VI and Tyrosine Kinase Syk in Thrombus Formation on Collagen-Like Surfaces. International Journal of Molecular Sciences, 20(11), 2788.

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Anemia Treatment Response Prediction

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The primary outcome was treatment response, defined as positive if an increase in hemoglobin of at least 5 g/L was observed within 2 months from randomization. Those remaining moderately anaemic and consenting to continue with the study were given the alternative treatment after month 2, with response assessed by month 4. Baseline variables were assessed for their ability to predict treatment response. Additional blood samples were taken at randomization for measurement of red cell parameters including reticulocyte hemoglobin using the XN‐9000 analyzer (Sysmex, UK) and hepcidin by enzyme‐linked immunosorbent assay (DRG International, New Jersey).

Hildebrand S., Busbridge M., Duncan N.D., Tam F.W, & Ashby D.R. (2022). Predictors of iron versus erythropoietin responsiveness in anemic hemodialysis patients. Hemodialysis International. International Symposium on Home Hemodialysis, 26(4), 519-526.

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Prognostic Factors in ICU Patients

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We collected each patient’s general information, APACHE II score, SIRS score, SOFA score, and CURB-65 score upon entering the ICU. Vital signs, blood tests, biochemical tests, coagulation analysis, and blood gas analysis were performed. We also collected each patient’s number of ICU hospitalization days and the prognosis when exiting the ICU. According to the different prognoses when leaving the ICU, we divided our cohort into the survival group and the mortality group. Blood routine testing was performed with a XN9000 analyzer (Sysmex Corporation, Japan). Biochemical analysis was performed with a Siemens ADVIA 2400 automatic biochemical analyzer. Brain natriuretic peptide (BNP) levels were measured with a DXi800 (Beckman Coulter, USA). Procalcitonin (PCT) was measured with a Cobas 8000 (Roche, Germany). C-reactive protein (CRP) levels were measured with an AU5800 (Beckman Coulter, USA). All detection reagents used the manufacturer’s matching reagents.

Wang H., Sun B., Li X., Wang Y, & Yang Z. (2022). Clinical analysis of severe COVID-19 patients. Technology and Health Care, 30(Suppl 1), 225-234.

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Comprehensive Biomarker Analyses in Clinical Samples

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Counter blood cell analyses were performed on XN 9000 analyzer (Sysmex Corporation, Kobe, Japan). Serum ALT, AST, ALP, LDH, GGT, creatinine, BUN, and uric acid measurements were conducted on Roche Cobas 8000 Modular Chemistry Analyzer (Roche Diagnostics, Indianapolis IN). Procalcitonin and IL‐6 tests were conducted on Coulter Access 2 (Beckman Coulter, Inc., Brea, CA, USA). CK‐MB, troponin, and ferritin measurements were conducted on Roche Cobas e411 (Roche Diagnostics, Indianapolis IN). Fibrinogen and D‐dimer were measured on ACL TOP 700 analyzer (Diamond Diagnostics Kft. Hungary).
Measurement of KYN metabolites by LCMSMS was done by modifying the method of Tömösi et al.²⁰

Cihan M., Doğan Ö., Ceran Serdar C., Altunçekiç Yıldırım A., Kurt C, & Serdar M.A. (2022). Kynurenine pathway in Coronavirus disease (COVID‐19): Potential role in prognosis. Journal of Clinical Laboratory Analysis, 36(3), e24257.

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Platelet Isolation from Healthy Volunteers

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Blood was obtained by venipuncture from healthy male and female volunteers who had not received anti-platelet medication for at least two weeks, after full informed consent according to the Helsinki declaration. The study was approved by the Medical Ethics Committee of Maastricht University. According to the approval, blood donor age and sex were not recorded. Blood samples were collected into 3.2% trisodium citrate (Vacuette tubes, Greiner Bio-One, Alphen a/d Rijn, The Netherlands). All subjects had normal platelet counts, as measured with a Sysmex XN-9000 analyzer (Sysmex, Kobe, Japan). Where indicated, platelet concentrates from unknown healthy subjects were obtained from Sanquin (Amsterdam, the Netherlands), after written permission from Sanquin and full informed consent. A concentrate unit contained >2.5 x10¹¹ platelets in 300 mL PAS-E solution and 30-35% plasma (Curvers et al., 2004 (link)). Concentrates were obtained at two days after blood drawing and were kept at room temperature by gently shaking.

Fernández D.I., Provenzale I., Cheung H.Y., van Groningen J., Tullemans B.M., Veninga A., Dunster J.L., Honarnejad S., van den Hurk H., Kuijpers M.J, & Heemskerk J.W. (2021). Ultra-high-throughput Ca2+ assay in platelets to distinguish ITAM-linked and G-protein-coupled receptor activation. iScience, 25(1), 103718.

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Comprehensive Metabolic and Hematologic Profiling

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All blood samples were collected with anticoagulant tubes in the morning after an overnight (12 h) fast during hospital stay. All biochemical analyses were conducted at the accredited hospital laboratories on the day of blood collection. Serum homocysteine levels were analyzed via the enzymatic cycling method. Serum levels of fasting glucose, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), total triglyceride (TG), lipoprotein (LPa), apolipoprotein A1 (Apo A1), apolipoprotein B (Apo B), creatinine, cystatin C, blood urea nitrogen (BUN), uric acid (UA), and albumin were determined with standard methods on an automatic biochemistry analyzer (Roche Cobas-c702 modular analyzer). Levels of hemoglobin, platelets and white blood cell (WBC) were determined using a Sysmex XN-9000 analyzer.

Shang K., Ning X., Kuang J., Xue A., Yan X, & Chen H. (2023). Hypertension and cystatin C account for sex differences in serum homocysteine levels in acute coronary syndrome subjects with normal serum creatinine. Journal of Health, Population, and Nutrition, 42, 81.

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Platelet Measurement and Yield Calculation

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Numbers of platelets, white (WBC) and red (RBC) blood cells were measured using a Sysmex XN-9000 analyzer (Sysmex, Kobe, Japan). By the same procedure, the following additional platelet parameters were recorded: mean platelet volume (MPV), which describes the platelet size, immature platelet fraction (IPF), which reflect the fraction of immature platelets, and platelet distribution width (PDW), which describes the variation in platelet size.
For some purposes the platelet yield, that is the total number of platelets in the sample, is of relevance, for instance in the process of isolating platelets, where a high platelet yield after the centrifugation is preferable. The platelet yield was calculated, using plasma density = 1025 mg/cm 3 , by the following equation:

Söderström A.C., Nybo M., Nielsen C, & Vinholt P.J. (2016). The effect of centrifugation speed and time on pre-analytical platelet activation. Clinical chemistry and laboratory medicine, 54(12).

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