Aicar

For egg yolk feeding, chicken eggs were obtained from local grocery stores, and the yolk was separated and diluted to 5% by volume with 0.3× Danieau solution as previously described (26 (link)). All drugs were made in 1,000× stock solution and stored in light-protected Eppendorf tubes at −20°C. NBI-31772 (5 mmol/L; Sigma-Aldrich), linsitinib (10 mmol/L; LC laboratories), H-89 (10 mmol/L; LC laboratories), AICAR (100 mmol/L; LC laboratories), afatinib (10 mmol/L; LC laboratories), SB431542 (10 mmol/L; Selleckchem), DAPT (10 mmol/L; Sigma-Aldrich), SU5402 (15 mmol/L; Calbiochem and Tocris), neocuproine (10 mmol/L; Sigma-Aldrich), PD0325901 (10 mmol/L; Sigma-Aldrich), U0126 (10 mmol/L; LC laboratories), and TUDCA (0.5 mol/L; Calbiochem) were dissolved in DMSO at the indicated concentrations. NVP-AEW541 (10 mmol/L; Cayman Chemicals), vatalanib (10 mmol/L; LC Laboratories), and SAG (10 mmol/L; EMD Millipore) were dissolved in water. CyA (10 mmol/L; LC Laboratories) was dissolved in ethanol.
Induction of transgene expression of Kir6.2^DN was performed as previously described (24 (link)). SU5402 was added after 16 h of the induction for an 8-h treatment.