Equine recombinant granulocyte monocyte colony stimulating factor gm csf

Experimental reagents were obtained from the following sources: equine recombinant granulocyte-monocyte colony stimulating factor (GM-CSF) was obtained from Kingfisher Biotech (Saint Paul, MN, USA); lipopolysaccharide (LPS) from E. coli 055:B5, bovine serum albumin (BSA), sodium dodecyl sulfate (SDS), sodium deoxycholate, NP-40, sodium pyrophosphate, sodium fluoride, phenylmethylsulfonyl fluoride (PMSF), and diisopropylfluorophosphate (DFP) were obtained from Sigma-Aldrich (St. Louis, MO, USA); goat polyclonal anti-mPGES-1, anti-COX-1, and anti-COX-2 antibodies and HRP-conjugated donkey anti-goat secondary antibodies were purchased from Santa Cruz Biotechnologies (Dallas, TX, USA); mouse monoclonal anti-cPGES antibody, MF63, NS-398, and indomethacin were from Cayman Chemical (Ann Arbor, MI, USA); rabbit polyclonal anti-β-Actin antibody and HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were from Cell Signaling (Danvers, MA, USA).