Atcc 700684

H. pylori reference strains ATCC 700392, ATCC 43504 (metronidazole-resistant), and ATCC 700684 (clarithromycin resistant) were purchased from the American Type Culture Collection (Rockville, MD, USA). The strains were routinely grown in Blood Agar Base No. 2 (OXOID, Basingstoke, UK) supplemented with 8% defibrinated horse blood (OXOID) in a humidified microaerobic incubator (85% N₂, 10% CO₂, 5% O₂) at 37 °C for 48–72 h. For certain experiments, bacteria were grown for 48–72 h at 37 °C in brain heart infusion broth (OXOID) supplemented with 4% foetal bovine serum (Gibco, Carlsbad, CA, USA).
Escherichia coli strain ATCC 25922 and Staphylococcus epidermidis strain ATCC 12228, obtained from the local culture collection of the Department of Microbiology, Preventive Medicine and Public Health of the University of Zaragoza (Spain), were used in some susceptibility evaluations. For this purpose, the strains were grown in Mueller-Hinton agar/broth (PanReac AppliChem, Barcelona, Spain) overnight at 37 °C.

H. pylori reference strains ATCC 700392, ATCC 43504 (metronidazole-resistant), and ATCC 700684 (clarithromycin resistant) were purchased from the American Type Culture Collection and used in the in vitro antibacterial assays. The strains were grown in Blood Agar Base No.2 (OXOID) supplemented with 8% defibrinated horse blood (OXOID) in a humidified microaerobic incubator (85% N₂, 10% CO₂, 5% O₂) at 37 °C for 48–72 h. For certain experiments, bacteria were grown for 48–72 h at 37 °C in brain heart infusion broth (OXOID) supplemented with 4% fetal bovine serum (Gibco). For in vivo experiments, the CagA⁺ mouse colonizing strain pre-mouse Sydney Strain 1 (PMSS1) [20 (link),21 (link)], obtained from the French National Reference Center for Campylobacters & Helicobacters (www.cnrch.fr), was used. For infection inocula, H. pylori PMSS1 was grown on in-house selective Wilkins Chalgren agar plates [22 (link)] under microaerobic conditions and transferred to Brucella broth medium as described below.

Helicobacter pylori reference strains ATCC 43504 (metronidazole-resistant) and ATCC 700684 (clarithromycin-resistant) were purchased from the American Type Culture Collection (Rockville, MD, United States). The H. pylori clinical isolate Donostia 2, resistant to levofloxacin, was isolated from gastroduodenal biopsies at Donostia University Hospital (San Sebastian, Spain). The H. pylori strain 26695 (ATCC 700392) was used in some experiments. All H. pylori strains were routinely grown in Blood Agar Base No. 2 (OXOID, Basingstoke, United Kingdom) supplemented with 8% defibrinated horse blood (OXOID) in a humidified microaerobic incubator (85% N₂, 10% CO₂, and 5% O₂) at 37°C for 48–72 h. For certain experiments, bacteria were grown for 48–72 h at 37°C in brain heart infusion broth (OXOID) supplemented with 4% fetal bovine serum (Gibco, Carlsbad, CA, United States).
Escherichia coli ATCC 25922 and Staphylococcus epidermidis ATCC 12228 were kindly provided by Jose Antonio Aínsa (University of Zaragoza), and belong to the microbial culture collection of the Department of Microbiology, from this university. Both strains were routinely cultured overnight in Mueller-Hilton agar/broth (PanReac AppliChem, Barcelona, Spain) at 37°C.