The CT26.WT cell line (murine colon cancer) was obtained from American Type Culture Collection (ATCC). The HaCaT cell line (human skin keratinocytes) was purchased from Cell Lines Service (CLS) [30 (link)]. The HT29 cell line (human colon cancer) was generously provided by Dr. Helleday (Karolinska Institute, Sweden). The HT29 and HaCaT cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) high glucose medium. The CT26.WT cell line was grown in RPMI 1640. All media were supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, and 10% fetal bovine serum. All cells were kept at 37 °C in a humidified atmosphere containing 5% CO2. Cell culture reagents were purchased from Biowest or Thermo Fisher Scientific.
Ct26 wt cell line
Manufactured by American Type Culture Collection
Sourced in United States
The CT26.WT cell line is a murine colon carcinoma cell line derived from a tumor induced in a BALB/c mouse. It is a widely used model for studying cancer biology and immunotherapy.
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2 protocols using ct26 wt cell line
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Cell Lines for Cancer Research
2
Mouse CT26.wt Cell Culture Protocol
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Mouse CT26.wt cell line was purchased from American Type Culture Collection (ATCC, USA). The cell line was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (ExCell Bio, China, Uruguay Origin) and 1% penicillin, streptomycin and amphotericin-B. All cells were incubated in a constant temperature incubator maintained at 37°C and containing 5% CO2.
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