HEK293T, HK-2, ACHN, 786-O, Caki-1, KETR3, and OS-RC2 cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). 786-O, Caki-1, KETR3, and OS-RC2 cells were cultured in RPMI-1640 medium (KGM31800NH-500, KeyGEN BioTECH) supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin and incubated in a 37◦C humidified incubator with 5% CO2. ACHN cells were cultured in MEM (KGM41500N-500, KeyGEN BioTECH), HEK293T, and HK-2 cells were cultured in DMEM (KGM12800NH-500, KeyGEN BioTECH), and the other cell culture conditions were the same as those described above.
Ketr 3
Manufactured by American Type Culture Collection
Sourced in United States
The Ketr-3 is a laboratory equipment designed for the cultivation and maintenance of cell cultures. It provides a controlled environment for the growth and preservation of various cell lines. The Ketr-3 ensures consistent temperature, humidity, and gas conditions required for optimal cell culture development.
Automatically generated - may contain errors
Lab products found in correlation
Caki 1, by American Type Culture Collection (2 mentions)
Osrc 2, by American Type Culture Collection (2 mentions)
Caki 2, by American Type Culture Collection (2 mentions)
Caki 1, by Keygen Biotech (1 mentions)
Os rc2, by Keygen Biotech (1 mentions)
Kgm41500n 500, by Keygen Biotech (1 mentions)
Kgm12800nh 500, by Keygen Biotech (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Ect1 e6e7, by Thermo Fisher Scientific (1 mentions)
Caki 1, by Thermo Fisher Scientific (1 mentions)
Emem medium, by Thermo Fisher Scientific (1 mentions)
Ketr 3, by Thermo Fisher Scientific (1 mentions)
Rt112, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ect1 e6e7, by American Type Culture Collection (1 mentions)
Rt112, by American Type Culture Collection (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
5 azac, by Merck Group (1 mentions)
4 protocols using ketr 3
1
Culture and Maintenance of Renal Cell Lines
2
Establishing RCC Cell Lines for Research
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Thirty pairs of RCC specimens and adjacent normal tissues were obtained from patients who underwent surgical treatment from October 2015 to August 2016 in Tai'an City Central Hospital. Written informed consent was obtained prior to resection from patients and the study was approved by the Ethics Committee of Tai'an City Central Hospital.
Cell lines. RCC cell lines 786-O, Caki-1, Ketr-3, RT112 and T24, as well as the normal renal epithelial cells Ect1/E6E7 were purchased from the American Type Culture Collection (ATCC, Gaithersburg MD, USA). The 786-O, Ketr-3, RT-112 and T24 cells were cultured in RPMI-1640 medium (Gibco, Rockville, MD, USA) containing 10% fetal bovine serum (FBS; Gibco). The Caki-1 cells were cultured in McCoy's 5A medium containing 10% FBS, and Ect1/E6E7 cells were cultured in EMEM medium (Gibco) containing 10% FBS. All cells were cultured in incubators at 37˚C in a 5% CO 2 atmosphere.
Cell lines. RCC cell lines 786-O, Caki-1, Ketr-3, RT112 and T24, as well as the normal renal epithelial cells Ect1/E6E7 were purchased from the American Type Culture Collection (ATCC, Gaithersburg MD, USA). The 786-O, Ketr-3, RT-112 and T24 cells were cultured in RPMI-1640 medium (Gibco, Rockville, MD, USA) containing 10% fetal bovine serum (FBS; Gibco). The Caki-1 cells were cultured in McCoy's 5A medium containing 10% FBS, and Ect1/E6E7 cells were cultured in EMEM medium (Gibco) containing 10% FBS. All cells were cultured in incubators at 37˚C in a 5% CO 2 atmosphere.
3
Cell Lines Maintenance for HDAC1 and SPOP
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All tumor cell lines including HCT-116, A498, 769-P, Caki-2, Ramos, OS-RC-2 and Ketr-3 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, United States). All cells were maintained in a humidified incubator with 5% CO2 and 95% air at 37 °C under standard conditions. Compounds were purchased from WuXi AppTec. HDAC1 and SPOP proteins were obtained from Abcam (Cambridge, MA, United States).
4
Demethylation of ccRCC cell lines
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Five ccRCC-derived cell lines (A498, Caki-2, Ketr-3, Osr, and 786-O) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) were prepared to validate the methylation status of ADAMTS18. The HEK293 human normal embryonic kidney cell line and HK-2 human kidney proximal tubular epithelial cell line were both routinely cultured and served as “normal” controls. All of these cell lines were maintained in dulbecco’s modified eagle medium supplemented with 2 mM glutamine and 10% fetal bovine serum at 37 °C with 5% CO2. The demethylating agent 5-AzaC (Sigma®, Hong Kong, China) was freshly prepared in ddH2O and filter sterilised. Then, the ccRCC-derived cell lines were demethylated by daily treatments with fresh medium containing 10 µM 5-AzaC for 3 days (doubling time: 36 h). The medium was changed every 24 h. After the treatment, the cells were pelleted and washed with PBS, and DNA/RNA was extracted.
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