3x106 SUDHL6 cells were injected subcutaneously into NSG mice
(Charles River Laboratories, Wilmington, MA, USA). After 17 days (when tumour
volume averaged 63mm3) mice were randomised into two groups (each
n=4) and treated orally with either vehicle (0.1% DMSO in 10%
2-hydroxypropyl-β-cyclodextrin; Cayman Chemical, MI, USA) or 6mg/kg
Siponimod (Selleckchem.com, Munich, Germany) every 48h. Mice were culled when
average tumour volumes in control mice reached 400mm3 (28 days).
Organs were weighed, minced and incubated with Liberase DL/Liberase TL and
DNASEI (Roche, Basel, Switzerland) [24 (link)].
Cell suspensions were labelled with mouse CD31 and CountBright absolute counting
beads (Thermofisher Scientific) and analysed by flow cytometry on LSRII and FACS
diva 8 (BD, Franklin Lakes, NJ, USA). Details of the other mouse models tested
are inSupplementary Materials
and Methods . All mouse experiments were done according to UK Home
Office guidelines.
(Charles River Laboratories, Wilmington, MA, USA). After 17 days (when tumour
volume averaged 63mm3) mice were randomised into two groups (each
n=4) and treated orally with either vehicle (0.1% DMSO in 10%
2-hydroxypropyl-β-cyclodextrin; Cayman Chemical, MI, USA) or 6mg/kg
Siponimod (Selleckchem.com, Munich, Germany) every 48h. Mice were culled when
average tumour volumes in control mice reached 400mm3 (28 days).
Organs were weighed, minced and incubated with Liberase DL/Liberase TL and
DNASEI (Roche, Basel, Switzerland) [24 (link)].
Cell suspensions were labelled with mouse CD31 and CountBright absolute counting
beads (Thermofisher Scientific) and analysed by flow cytometry on LSRII and FACS
diva 8 (BD, Franklin Lakes, NJ, USA). Details of the other mouse models tested
are in
and Methods
Office guidelines.