Human colorectal cancer cell line HCT-116 (ATCC, United States) was cultured in 1640 medium and LoVo (ATCC, United States) was cultured in F-12K medium, containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 mg/ml streptomycin at 37°C in a 5% CO2 incubator. Rabbit monoclonal antibodies against human E-cadherin, N-cadherin, Vimentin, Snail, β-arrestin1, GSK-3β, β-catenin, c-Myc, and CyclinD1 were purchased from Cell Signaling Technology (United States). Rabbit monoclonal antibodies against human MMP2 and MMP9 were purchased from Santa Cruz Biotechnology (United States). Mouse monoclonal antibody against human GAPDH was purchased from Proteintech (China). Tanshinone IIA (S2365) with 99% purity (HPLC) was purchased from Selleck (Houston, TX, United States).
β arrestin1
Manufactured by Cell Signaling Technology
Sourced in United States
β-arrestin1 is a protein that functions in the regulation of G protein-coupled receptor (GPCR) signaling. It plays a role in desensitizing GPCRs and facilitating receptor internalization.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin d1, by Cell Signaling Technology (2 mentions)
Rabbit monoclonal antibodies against human mmp2 and mmp9, by Santa Cruz Biotechnology (2 mentions)
C myc, by Cell Signaling Technology (2 mentions)
Hct116, by American Type Culture Collection (2 mentions)
Snail, by Cell Signaling Technology (2 mentions)
Gsk3β, by Cell Signaling Technology (2 mentions)
β catenin, by Cell Signaling Technology (2 mentions)
N cadherin, by Cell Signaling Technology (2 mentions)
Vimentin, by Cell Signaling Technology (2 mentions)
Control sirna, by Cell Signaling Technology (1 mentions)
β arrestin 2, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Pdgf b, by Abcam (1 mentions)
Mapk 42 44, by Cell Signaling Technology (1 mentions)
β arrestin2, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Phospho p38, by Cell Signaling Technology (1 mentions)
Odyssey scanner, by LI COR (1 mentions)
Tanshinone iia, by Selleck Chemicals (1 mentions)
5 protocols using β arrestin1
1
Tanshinone IIA Regulates Epithelial-Mesenchymal Transition in Colorectal Cancer Cells
2
Silencing β-Arrestin Proteins in Cells
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For siRNA-induced silencing of β-arrestins, β-arrestin1 (#6218S; Cell Signaling Technology, Danvers, MA, USA), β-arrestin2 (#sc-29743; Santa Cruz Biotechnology, Dallas, TX) or control siRNA (#6568S; Cell Signaling Technology) each at 25 mM were transfected using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's protocol.
3
Antibody Detection in Cell Signaling
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Antibodies against β-arrestin-1 and β-arrestin-2 (Cell signaling, Danvers, MA, USA), PDGF-β (Abcam), β-actin (Sigma), MAPK 42/44 (Cell Signaling), and phosphorylated MAPK 42/44 (Cell Signaling) were used at a 1:1000 dilution for the western blot analysis and at a 1:500 dilution for immunofluorescence.
4
Western Blotting Methodology for Protein Analysis
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The proteins separated by SDS–PAGE were transferred to a nitrocellulose membrane for western blotting using the procedure outlined previously [29 (link)]. The commercially obtained primary antibodies used in this study were against MK2 (Cell Signaling Technology, Cat. no. 3042), P38 (Cell Signaling Technology, Cat. no. 9212), phospho-P38 (Cell Signaling Technology, Cat. no. 9215), p44/42 ERK MAPK (Cell Signaling Technology, Cat. no. 4696), β-Arrestin1 (Cell Signaling Technology, Cat. no. 4674) and GST (Cell Signaling Technology, Cat. no. 2624). The antibodies to PDE4A5, PDE4B, PDE4C, PDE4D and phospho-PDE4A5 were raised by the Baillie Laboratory and have been described previously [18 (link),22 (link),29 (link)]. Arrestin and ERK were detected concomitantly during competition overlay experiments on peptide arrays using the Licor Odyssey Scanner as previously described [16 (link)].
5
Colorectal Cancer Cell Line Characterization
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Human colorectal cancer cell line HCT-116 (ATCC, USA) was cultured in 1640 medium and LoVo (ATCC, USA) was cultured in F-12K medium, at 37°C in a 5% CO 2 incubator, containing 10% fetal bovine serum (FBS), 100U/mL penicillin, 100mg/mL streptomycin. Rabbit monoclonal antibodies against human Ecadherin, N-cadherin, Vimentin, Snail, β-arrestin1, GSK-3β, β-catenin, c-Myc and CyclinD1 were purchased from Cell Signaling Technology (USA). Rabbit monoclonal antibodies against human MMP2 and MMP9 were purchased from Santa Cruz Biotechnology (USA). Mouse monoclonal antibodies against human GAPDH were purchased from Proteintech (China). Tanshinone IIA was purchased from Selleck (Houston, TX, USA).
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