Hep3B and HCT116 cells were purchased from BCRC (Food Industry Research and Development Institute, Taiwan). Hep3B cells were maintained in Dulbecco’s modified Eagle’s medium (high glucose, 1954626, Gibco). HCT116 cells were maintained in RPMI 1640 medium (31800022, Gibco). GFP and GFP::Npas4 were transfected into Hep3B and HCT116 by PolyJet and Lipofectamine 2000, respectively, according to the manufacturers’ instructions. For IL-6 treatment, cells were starved for 18 hours by maintaining them on serum-free medium before being subjected to IL-6 (25 ng/ml; GF338, Millipore) treatment at the indicated time points.
Hep3b cells
Manufactured by Thermo Fisher Scientific
Sourced in United States
Hep3B cells are a widely used human hepatocellular carcinoma cell line derived from a hepatocellular carcinoma tumor. They are a commonly used in vitro model for studying liver biology and disease. Hep3B cells retain many of the morphological and functional characteristics of primary human hepatocytes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Hct116, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Gf338, by Merck Group (1 mentions)
Nucleosides, by Thermo Fisher Scientific (1 mentions)
Cos 7, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Cos 7, by American Type Culture Collection (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
C11095500bt, by Thermo Fisher Scientific (1 mentions)
Ht 29, by Thermo Fisher Scientific (1 mentions)
C11995500bt, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
7 protocols using hep3b cells
1
Transient Transfection and IL-6 Treatment
2
Cell Line Authentication and Culture
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PC-3, COS-7 and Hep3B cells were obtained from the American Type Culture Collection (ATCC) and were authenticated by short-tandem repeat DNA profiling by Genetica. PC-3 and COS-7 cells were cultured in Dulbecco's modified Eagle medium supplemented with 10% foetal calf serum, while Hep3B cells were cultured in minimum essential medium (MEM)-α medium with GlutaMAX supplement and without nucleosides (Gibco).
3
Culturing Hep3B Cells under Normoxic and Hypoxic Conditions
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Human hepatocellular carcinoma Hep3B cells (ATCC) were maintained in RPMI 1640 medium (Gibco) containing 10% FBS and Penicillin/Streptomycin. Cell cultures were kept under standard normoxic conditions (21% O2) at 37 °C in a humidified atmosphere with 5% CO2. Cells were harvested by trypsination and subcultured in a ratio of 1:5–1:10.
For hypoxic exposure cells were incubated at 37 °C in a humidified atmosphere with 3% O2, 5% CO2 and balanced N2 for 5 or 8 h depending on type/purpose of the experiment.
For hypoxic exposure cells were incubated at 37 °C in a humidified atmosphere with 3% O2, 5% CO2 and balanced N2 for 5 or 8 h depending on type/purpose of the experiment.
4
Cell Culture Protocols for Hepatocellular Carcinoma
5
Culturing Human Cancer Cell Lines
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The human breast adenocarcinoma cell line MCF-7 (ATCC) and human renal carcinoma cells 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl (all described in [28 (link)]) were maintained in DMEM high glucose medium (Gibco®) supplemented with 10 % fetal bovine serum (FBS, Gibco®) and Penicillin/Streptomycin. Human hepatocellular carcinoma Hep3B cells (ATCC) were cultured in RPMI 1640 medium (Gibco®) supplemented with 10 % FBS and Penicillin/Streptomycin. Cell cultures were maintained at 37 °C in a humidified atmosphere containing 5 % v/v CO2. Cells were harvested by trypsinization and subcultured at least twice a week in a ratio of 1:5 – 1:10.
6
Cell Culture and Transfection Protocol
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HT29 was purchased from the American Type Culture Collection (ATCC; Manassas, USA), and HCT-116, H4, and Hep3B cells were purchased from Cell Bank of the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The H4 cells was cultured in Dulbecco’s modified Eagle’s medium (DMEM; C11995500BT; Gibco, Grand Island, USA), the Hep3B cells were cultured in Minimum Essential Medium (MEM; C11095500BT; Gibco), and the HT-29 and HCT-116 cells were cultured in McCoy′s 5A Medium (16600082; Gibco), all supplemented with 10% FBS (10091148; Gibco) and 1% penicillin/streptomycin (15140-122; Gibco) at 37°C in a humidified 5% CO
2 atmosphere. The cells were grown on coverslips in 35-mm diameter culture dishes until they reached approximately 70%-80% confluence. Then, the cells were transfected with the indicated plasmids utilizing Lipofectamine 3000 (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions.
2 atmosphere. The cells were grown on coverslips in 35-mm diameter culture dishes until they reached approximately 70%-80% confluence. Then, the cells were transfected with the indicated plasmids utilizing Lipofectamine 3000 (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions.
7
Cell Culture and Transient Transfection
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Hep3B cells (Cat: HB-8064) were purchased from American Type Culture Collection (ATCC, Manassas, USA). Hep3B cells were cultured in ATCC-formulated Eagle's Minimum Essential Medium (Cat: 30-2003, Manassas, USA) plus 10% fetal bovine serum (FBS, Gibco, Rockville, USA) and 50 μg/ml penicillin/ streptomycin (P/S, Gibco, Rockville, USA). Huh1 (Cat: JCRB0199) cells were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan), and were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco, Rockville, USA) plus 10% FBS and 50 μg/ml P/S. Cells were maintained in an incubator at 37 °C with 5% CO2. For transient transfection, PolyJet reagent (SL100688, Signagen, USA) was used following the manufacturer's instructions.
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