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Anti goat hrp dab cell tissue staining kit

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The Anti-Goat HRP-DAB Cell & Tissue Staining Kit is a tool for the immunohistochemical detection of goat-derived antigens in cell and tissue samples. The kit utilizes a horseradish peroxidase (HRP)-based system and 3,3'-diaminobenzidine (DAB) as the chromogenic substrate to visualize the target antigens.

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Lab products found in correlation

Polyclonal goat anti mouse ccl11 antibody, by R&D Systems (2 mentions) Xylene, by Thermo Fisher Scientific (2 mentions) Goat antimouse trop2, by R&D Systems (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) Dp72 ccd camera, by Olympus (1 mentions) Af1320, by R&D Systems (1 mentions) Bx51 microscope, by Olympus (1 mentions) Ihc zinc fixative, by BD (1 mentions) Safefix, by Thermo Fisher Scientific (1 mentions)

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Anti-goat HRP-DAB Cell and Tissue staining kit HRP‐DAB Cell & Tissue Staining Kit

8 protocols using anti goat hrp dab cell tissue staining kit

1

Kidney Tissue Staining Protocol

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Formalin fixed kidney sections (4 μm) were deparaffinized in xylene and rehydrated through a graded ethanol series to water. After blocking with 10% normal horse serum in PBS, the tissue was stained with rat NGAL and KIM-1 polyclonal antibody overnight at 4°C and then was stained with the Anti-Goat HRP-DAB Cell&Tissue Staining Kit (R&D system, Minneapolis, MN, USA) and counterstained with hematoxylin.
Xie Y., Xiao J., Fu C., Zhang Z., Ye Z, & Zhang X. (2018). Ischemic Preconditioning Promotes Autophagy and Alleviates Renal Ischemia/Reperfusion Injury. BioMed Research International, 2018, 8353987.
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2

Immunohistochemical Analysis of CCL11

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Paraffin-embedded hearts were cut into 4-μm-thick sections. After deparaffinization, heat-induced antigen retrieval and blocking, sections were stained with 5 μg/ml Polyclonal Goat Anti-Mouse CCL11 antibody (R&D Systems) and Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems) and counter stained with Hematoxylin.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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3

Tumor Necrosis and Angiogenesis Quantification

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Two days after the therapy, 3–4 mice from each experimental group were sacrificed and the tumors were excised. The tumors were fixed in IHC zinc fixative (BD Biosciences) and embedded in paraffin. Two consecutive 2-μm thick sections were cut from each paraffin block. The first section was used to estimate the percent of necrosis by 4 independent observers after staining with hematoxylin and eosin. The second section was used for immunohistochemical (IHC) staining of blood vessels. It was incubated with primary goat polyclonal antibodies against mouse CD105 (AF1320, R&D Systems, Minneapolis, MN, USA) at dilution 1:1800. As the colorogenic reagent, a peroxidase-conjugated streptavidin–biotin system (CTS008, Anti-Goat HRP-DAB Cell & Tissue Staining Kit, R&D Systems) was used and followed by hematoxylin counterstaining.
The IHC stained slides were observed under light microscopy and at least 5 images of viable tumor tissue from each slide were captured with a DP72 CCD camera (Olympus) connected to a BX-51 microscope (Olympus) under 40x magnification (numerical aperture 0.85). On the acquired images the number of CD105 positive blood vessels was determined.
Stimac M., Dolinsek T., Lampreht U., Cemazar M, & Sersa G. (2015). Gene Electrotransfer of Plasmid with Tissue Specific Promoter Encoding shRNA against Endoglin Exerts Antitumor Efficacy against Murine TS/A Tumors by Vascular Targeted Effects. PLoS ONE, 10(4), e0124913.
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4

Quantifying Eosinophil Infiltration in Mouse Hearts

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Mouse hearts were cut in half, fixed in SafeFix (Thermo Fisher Scientific), paraffin embedded, and cut into 5-μm-thick sections (Histoserv). Serial sections were stained with 5 μg/mL polyclonal goat anti-mouse CCL11 or CCL24 antibodies (R&D Systems) using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems) and counterstained with Hematoxylin. The number of infiltrating eosinophils was determined by counting eosinophils on 40×images of H&E-stained biopsy sections. The number of eosinophils was averaged over the area observed.
Diny N.L., Hou X., Barin J.G., Chen G., Talor M.V., Schaub J., Russell S.D., Klingel K., Rose N.R, & Čiháková D. (2016). Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart. European journal of immunology, 46(12), 2749-2760.
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5

Immunohistochemical Analysis of CCL11

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Paraffin-embedded hearts were cut into 4-μm-thick sections. After deparaffinization, heat-induced antigen retrieval and blocking, sections were stained with 5 μg/ml Polyclonal Goat Anti-Mouse CCL11 antibody (R&D Systems) and Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems) and counter stained with Hematoxylin.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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6

Immunohistochemical Analysis of Mouse Prostate

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3-month-old and 24-month-old mouse prostate tissue was embedded in paraffin and sectioned at UCLA’s Translational Pathology Core Laboratory. Sections were incubated at 60°C in a vacuum oven for 45-60 min. Slides were transferred into xylene (Fisher) 3 times, 100% alcohol (Decon Labs) 2 times, 95% ethanol 1 time and 70% ethanol 1 time, each for 3 min. Slides were transferred into PBS (GIBCO) for 5 min prior to epitope unmasking using a heat antigen retrieval step. Staining of sections was performed using the manufacturer’s protocol for the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R & D Systems) with primary antibody goat antimouse Trop2 (R & D Systems) at a 10 μg/ml concentration. Hematoxylin and eosin staining was performed by UCLA’s Translational Pathology Core Laboratories. Frozen sections were fixed with 4% paraformaldehyde in PBS for 5 min at room temperature, washed with PBS, and stained with primary antibodies and stained with the following secondary antibodies: goat anti-mouse IgG-Alexa Fluor 488 and goat anti-rabbit IgG-Alexa Fluor 594.
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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7

Immunohistochemical Analysis of Mouse Prostate

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3-month-old and 24-month-old mouse prostate tissue was embedded in paraffin and sectioned at UCLA’s Translational Pathology Core Laboratory. Sections were incubated at 60°C in a vacuum oven for 45-60 min. Slides were transferred into xylene (Fisher) 3 times, 100% alcohol (Decon Labs) 2 times, 95% ethanol 1 time and 70% ethanol 1 time, each for 3 min. Slides were transferred into PBS (GIBCO) for 5 min prior to epitope unmasking using a heat antigen retrieval step. Staining of sections was performed using the manufacturer’s protocol for the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R & D Systems) with primary antibody goat antimouse Trop2 (R & D Systems) at a 10 μg/ml concentration. Hematoxylin and eosin staining was performed by UCLA’s Translational Pathology Core Laboratories. Frozen sections were fixed with 4% paraformaldehyde in PBS for 5 min at room temperature, washed with PBS, and stained with primary antibodies and stained with the following secondary antibodies: goat anti-mouse IgG-Alexa Fluor 488 and goat anti-rabbit IgG-Alexa Fluor 594.
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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8

Immunohistochemical Detection of CCL3 in Paraffin Sections

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Paraffin sections were prepared in the same manner as that for H&E staining. Antigen retrieval was performed according to the primary antibody instructions. An appropriate amount of endogenous peroxidase blocker was added to the sample, which was incubated at room temperature for 10 min and then rinsed with PBS. Goat anti-mouse CCL3 antibody (1:40, AF-450-SP) was added to the specimen and incubated at 37°C for 60 min. The tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; R&D Systems, CTS008) and counterstained with hematoxylin (blue).
Yuan X., Xiong Z., Liu W., Li Y., Li H., Zhang X., Yin Y., Xu P., Cao J., Chen D, & Song Z. (2022). Novel Therapeutic Targeting of CCL3-CCR4 Axis Mediated Apoptotic Intesitnal Injury in Necrotizing Enterocolitis. Frontiers in Immunology, 13, 859398.
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