Pegdma

Manufactured by Polysciences

Sourced in United States

PEGDMA is a polyethylene glycol-based dimethacrylate crosslinking agent. It is commonly used in the synthesis of hydrogels and other polymer-based materials for various applications.

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Polyethylene glycoldimethacrylate(PEGDMA, (3 citations)

10 protocols using pegdma

Fabrication of Photocrosslinkable Microgels

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PEG precursor solution was prepared by dissolving (20%, wt/wt) Poly(ethylene glycol) dimethacrylate (PEGDMA; MW 1000; Polysciences) in Dulbecco’s phosphate-buffered saline (DPBS, GIBCO). GelMA precursor solution was prepared by dissolving (5%, wt/wt) gelatin methacrylate (GelMA) foam-like powder in Dulbecco’s phosphate-buffered saline (DPBS, GIBCO). Then, 2-hydroxy-1-(4-(hydroxyethoxy)phenyl)-2-methyl-1-propanone photoinitiator (1%, wt/wt, Irgacure 2959; CIBA Chemicals) was added to the prepolymer solution. In this study, we used photomasks with several geometries (Fig. 2). A 50 μl droplet of photocrosslinkable prepolymer solution was pipetted onto a glass slide covered by a cover slip and separated by spacers (cover slip 25 × 25 mm², thickness: 150 μm). The photomasks were placed on the cover slip between the UV light and prepolymer. Another 25 × 25 mm² coverslide was placed onto the droplet. Microgels were fabricated by exposing the gel prepolymer solution to UV light (500 mW; at a height of 50 mm above the microgels) for 30 seconds, for polymerization to take place on the surface of the glass slide. Then, the photomask and glass cover slip were removed. Using a scalpel, the microgels were removed and left to soak in DPBS solution in a standard 60 mm Petri dish (Fisher Scientific).

Tasoglu S., Diller E., Guven S., Sitti M, & Demirci U. (2014). Untethered micro-robotic coding of three-dimensional material composition. Nature communications, 5, 3124.

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Hydrogel Synthesis and Characterization

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PEGDMA (MW: 1000) was purchased from Polysciences (Warrington, PA, USA). Edible pigments were purchased from Lgreentech (Daejeon, Gyeonggi, South Korea). Penicillin/streptomycin (P/S) and phosphate buffered saline (PBS, pH 7.4) were purchased from WelGene (Daegu, Gyeongbuk, South Korea). Gelatin (Type A, 300 bloom from porcine skin), methacrylic anhydride (MA), and LAP (≥95%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemical agents used in this study were of analytical grade.

Seo J.W., Kim G.M., Choi Y., Cha J.M, & Bae H. (2022). Improving Printability of Digital-Light-Processing 3D Bioprinting via Photoabsorber Pigment Adjustment. International Journal of Molecular Sciences, 23(10), 5428.

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Fluorescence-Preserving BB-PEG Formulation

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BB-PEG was prepared from mixing 75% v/v benzyl benzoate (BB) (Sigma-Aldrich B6630) and 25% v/v PEGMMA500 (Sigma-Aldrich 409529) supplemented with 3% w/v Quadrol (Sigma-Aldrich 122262) together. The fresh medium was a colorless liquid with low viscosity and turned slightly yellow in a week. Other forms of PEGs including PEGMMA200 (Polysciences 16664), PEGDA400 (Polysciences, 01871), PEGDMA (Polysciences, 00096), PEG200 (Sigma-Aldrich 81150), PEG400 (Sigma-Aldrich 807485), and PEG1000 (Sigma-Aldrich 81190) were also used for comparing their fluorescence preservation capabilities.

Jing D., Zhang S., Luo W., Gao X., Men Y., Ma C., Liu X., Yi Y., Bugde A., Zhou B.O., Zhao Z., Yuan Q., Feng J.Q., Gao L., Ge W.P, & Zhao H. (2018). Tissue clearing of both hard and soft tissue organs with the PEGASOS method. Cell Research, 28(8), 803-818.

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Synthesis of PEGDMA Hydrogels

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Polyethylene glycol dimethacrylate (PEGDMA) was acquired from Polysciences Inc. with average molecular weights of 1000 and 8000 Dalton (PEGDMA 1000 and PEGDMA 8000). Iron chloride tetrahydrate, iron chloride hexahydrate, sodium hydroxide pellets and 2,2-dimethoxy-2-phenylacetophenone (DMPA) were purchased from Sigma Aldrich.

Oberdick S.D., Russek S.E., Poorman M.E, & Zabow G. (2020). Observation of iron oxide nanoparticle synthesis in magnetogels using magnetic resonance imaging. Soft matter, 16(45), 10244-10251.

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Fabrication of Photocrosslinkable Microgels

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Tasoglu S., Diller E., Guven S., Sitti M, & Demirci U. (2014). Untethered micro-robotic coding of three-dimensional material composition. Nature communications, 5, 3124.

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Dual Hydrogel Scaffold Fabrication

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The dual hydrogel culture system was fabricated on the membranes of Transwell ® 6-well inserts (0.4 µm/PES; Corning) using digital projection photolithography as previously described (Curley and Moore, 2011; (link)Bowser and Moore, 2019) (link). The outer cell-impermeable hydrogel was created using a photo-translinkable solution of 10% w/v polyethylene glycol dimethacrylate 1000 (PEGDMA; Polysciences, Warrington, PA) and 0.55 mM lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP; Sigma-Aldrich, St. Louis, MO). The photo-translinkable solution was irradiated for 25-30 sec using ultraviolet light of 385 nm wavelength at 300 W/m 2 with a digital micromirror device (DMD) light engine (PRO4500 Wintech Digital Systems Technology Corp, Carlsbad, CA). A mask design and polymerization parameters were selected using commercial software (DLP Lightcrafter 4500 Control Software, Texas Instruments, Dallas, TX). The mask shape resembles a keyhole, where the circular reservoir is referred to as the "bulb" and the narrow slot is the "channel". The constructs were washed using 2% antibiotic/antimycotic wash buffer (Thermo Fischer Scientific, Walton, MA) and the channels were filled with 8% Growth Factor-Reduced Matrigel ® Matrix (Corning, Corning, NY) to create a cell-permeable scaffold.

Kramer L., Nguyen H.T., Jacobs E., McCoy L., Curley J.L., Sharma A.D, & Moore M.J. (2020). Modeling chemotherapy-induced peripheral neuropathy using a Nerve-on-a-chip microphysiological system. ALTEX, 37(3).

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Zwitterionic Hydrogel Synthesis and Characterization

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Photoinitiator
2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (HEPK,
Sigma-Aldrich) was dissolved into deionized water to obtain an approximately
0.077 wt % solution. The two zwitterionic monomers used (chemical
structures shown in Figure 1) were sulfobetaine methacrylate (SBMA, 2-methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium
hydroxide, Sigma-Aldrich) and carboxybetaine methacrylate (CBMA, 3-[[2-(methacryloyloxy)ethyl]-
dimethylammonio]propionate, TCI Chemicals). Poly(ethylene glycol)
methacrylate (PEGMA, 400 g/mol, Polysciences) and 2-hydroxyethyl methacrylate
(HEMA, Sigma-Aldrich) were used as nonzwitterionic monomer controls.
Poly(ethylene glycol) dimethacrylate (PEGDMA, 400 g/mol, Polysciences)
was used as the cross-linker (chemical structure shown in Figure 1) for all monomer
systems. Monomer and cross-linker were added at various ratios totaling
35 wt % of the prepolymer solution, with the remaining 65 wt % composed
of the water/HEPK mixture. Monomer solutions and resultant hydrogel
films are identified with the percent of the total monomer that is
PEGDMA (cross-linker).

Peel A., Bennion D., Horne R., Hansen M.R, & Guymon C.A. (2024). Photografted Zwitterionic Hydrogel Coating Durability for Reduced Foreign Body Response to Cochlear Implants. ACS Applied Bio Materials, 7(5), 3124-3135.

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Fabrication of Biomimetic Nerve Scaffolds

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Collagen (type IV, 0.5–2 mg ml⁻¹) (Sigma-Aldrich, USA), acetic acid (Sigma-Aldrich, USA), trypsin (Gibco, USA), donor horse serum (DHS) (Capricorn, Germany), fetal bovine serum (FBS) (Sigma-Aldrich, USA), l-glutamine (Gibco, USA), gentamicin (Gibco, USA), RPMI-1640 (Sigma-Aldrich, USA), nerve growth factor (NGF) (Vipera lebetina venom) (Sigma-Aldrich, USA), phosphate-buffered saline (PBS) (Sigma Aldrich, USA), NaOH (Sigma-Aldrich, USA), Irgacure (Sigma-Aldrich, USA), PEGDMA (PolyScience, USA), MWCNTs (Nanografi, Turkey), 3-(trimethoxysilyl)propyl methacrylate (TMSPM) (Sigma-Aldrich, USA), AZ5214E (MicroChemicals, Germany), Calcein-AM Cell Viability Assay Kit (Biotium, USA), Blood/Cell Total RNA Mini Kit (Geneaid, Sijhih City, Taiwan), M-MuLV First Strand cDNA Synthesis Kit (Biomatik, Ontario, Canada).

Seven F., Gölcez T., Yaralı Z.B., Onak G., Karaman O, & Şen M. (2020). Guiding neural extensions of PC12 cells on carbon nanotube tracks dielectrophoretically formed in poly(ethylene glycol) dimethacrylate. RSC Advances, 10(44), 26120-26125.

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PEG-based Hydrogel Coating for MEAs

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The 5 wt% of Poly(ethylene glycol) dimethacrylate (PEGDMA, M_n 20,000, 25406-5, Polysciences), 5 wt% of PEGDMA (M_n 550, 409510, Sigma), and 0.5 wt% of 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone photoinitiator (IRGACURE 2959, 410896, Sigma) were mixed in PBS and ultrasonicated until complete dissolution. MEAs were dipped in this solution for 3 s and then exposed to UV light (365 nm, Thorlabs) for 15 min. Afterward, MEAs were stored inside the hardening solution (1 wt% of CaCl₂ in PBS).

Ferlauto L., D’Angelo A.N., Vagni P., Airaghi Leccardi M.J., Mor F.M., Cuttaz E.A., Heuschkel M.O., Stoppini L, & Ghezzi D. (2018). Development and Characterization of PEDOT:PSS/Alginate Soft Microelectrodes for Application in Neuroprosthetics. Frontiers in Neuroscience, 12, 648.

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Fabrication and Characterization of Ocular Biomaterials

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PEGDMA was purchased from Polysciences (Warrington, PA, USA). PEGDA, Irgacure 2959, benzophenone, FITC labeled dextran-40 kDa, PLGA, dexamethasone, chitosan were purchased from Sigma-Aldrich (St. Louis, MO, USA). FITC-PLGA was purchased from Nanosoft Biotechnology (Lewisville, NC, USA). Acrylic sheets and very-high-bond (VHB) foam tape were purchased from Mcmaster-Carr (Robbinsville, NJ, USA). Bevacizumab solution (Avastin) was purchased from Genentech, Inc (San Francisco, CA, USA). Anti-bevacizumab antibodies (HCA 182 and HCA184P), HISPEC Assay Diluent (BUF049) were purchased from Bio-Rad (Raleigh, NC, USA). The standard ABTS ELISA development kit was purchased from Peprotech (Rochy Hill, NJ, USA). Retinal pigmented epithelium cells (RPE-19, ATCC CRL-2302), choroidal/retina endothelial cells (RF/6A, ATCC CRL-1780), primary corneal epithelial cells (Human Pr. HCEC, ATCC PCS-700–010), and corneal endothelial cells (BCE C/D-1b, ATCC CRL-2048) were purchased from ATCC (Manassas, Virginia, USA).

Zhao F., Fan S., Ghate D., Romanova S, & Zhao S. (2021). A hydrogel ionic circuit-based high-intensity iontophoresis device for intraocular macromolecules and nanoparticles delivery. Advanced materials (Deerfield Beach, Fla.), 34(5), e2107315.

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