Thirteen human gastric cancer cell lines were used in this study (Table S1 ) and all cultures maintained at 37 °C in the presence of 5% CO2. SNU5 (IMDM + 20% FBS); MKN45, NCI-N87, SNU16, HGC27, AGS, NUGC3, SNU1 and NUGC4 (RPMI 1640 + 10% FBS); KATOIII (IMDM + 20% FBS); MGC803 and BGC823 (DMEM + 10% FBS). Abemaciclib (#S5716) and JQ1(#S7110) were obtained from SelleckChem LLC (Houston,TX). Ki-67 Rabbit mAb (#15,580) was purchased from Abcam. HRP conjugated Goat Anti-Rabbit IgG (H + L) secondary antibody (Servicebio Cat#GB23303, RRID:AB_2811189) was purchased from Servicebio. Cell Titer-Glo® (#G7570) was obtained from Promega. Crystal violet was obtained from Sigma. Antibodies against p21 (#2947T), p53 (#2524S), Phospho-Rb (Ser807/811) (#8516T), 53BP1 (#88,439) were purchased from Cell Signaling Technology (Danvers, MA). Anti- γH2AX (Ser139) (#05-636) was purchased from Millipore.
Ki 67 rabbit mab
Manufactured by Abcam
Ki-67 Rabbit mAb is an antibody used for the detection of the Ki-67 protein, a cellular marker for proliferation. The antibody is designed for use in immunohistochemistry (IHC) and other immunoassay techniques to identify and quantify Ki-67 expression in various cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet, by Merck Group (1 mentions)
Anti γh2ax ser139, by Merck Group (1 mentions)
Hrp conjugated goat anti rabbit igg h l secondary antibody, by Wuhan Servicebio Technology (1 mentions)
Antibodies against p21, by Cell Signaling Technology (1 mentions)
Celltiter glo, by Promega (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Gapdh rabbit mab, by Abcam (1 mentions)
Click it tunel colorimetric ihc detection kit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Akt pan c67e7 rabbit mab, by Cell Signaling Technology (1 mentions)
P akt ser473 rabbit mab, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 rabbit mab, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
2 protocols using ki 67 rabbit mab
1
Gastric Cancer Cell Lines: Protocols
2
Trastuzumab-Loaded PLGA Nanoparticles for Anti-HER2 Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Trastuzumab (Herceptin) was provided by Genentech and used directly. Meo-PEG5k-Dlinkm-PLGA11k copolymer and G0-C14 were synthesized using our published methods54 (link), 55 , 56 (link). PTEN mRNA, EGFP mRNA, and fluorescent dye Cy5-labeled mRNA (Cy5-EGFP mRNA and Cy5-PTEN mRNA) were purchased from APExBIO (Houston, TX, USA). PTEN rabbit mAb (#ab32199), HER2 rabbit mAb (ab214275), Ki67 rabbit mAb (#ab92742), and GAPDH rabbit mAb (#ab181602) were purchased from Abcam. Anti-rabbit IgG horseradish peroxidase (HRP)-linked secondary mAb (#7074), Akt (Pan) (C67E7) rabbit mAb (#4691), p-Akt (Ser473) rabbit mAb (#4060), and cleaved caspase-3 rabbit mAb (#9664) were purchased from Cell Signaling Technology (CST). Alexa Fluor 647-conjugated secondary antibody (#A-11011) and Click-iT™ TUNEL Colorimetric IHC Detection Kit (#C10625) were obtained from ThermoFisher. Fetal bovine serum (FBS), penicillin-streptomycin, Dulbecco's modified Eagle medium (DMEM), and trypsin were obtained from Invitrogen Corp. All solvents and reagents are of analytical grade and used directly.
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