Nupage 4 12 bis tris gradient precast gels

Manufactured by Thermo Fisher Scientific

The Nupage 4–12% Bis-Tris gradient precast gels are polyacrylamide gel electrophoresis (PAGE) products designed for the separation and analysis of proteins. These gels feature a gradient of acrylamide concentrations, allowing for the effective separation of a wide range of protein sizes.

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Lab products found in correlation

Protease and phosphatase inhibitor, by Roche (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Anti rabbit igg secondary antibody, by GE Healthcare (1 mentions) Vinculin, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Ptk2 fak, by Cell Signaling Technology (1 mentions) Ha tag, by Cell Signaling Technology (1 mentions) V5 tag, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

NuPAGE 4–12% Bis-Tris gel (1261 citations) NuPAGE 4–12% Bis-Tris gradient gels (139 citations) NuPAGE Bis-Tris precast gels (95 citations) NuPAGE 12% Bis-Tris gels (60 citations) NuPage 4–12% Bis-Tris precast gels (57 citations) NuPAGE Bis-Tris gradient gels (56 citations) NuPAGE 4–12% gradient precast gels (4 citations) NuPage precast 4–12% Bis-Tris gels (3 citations) NuPAGE bis-tris precast gradient gels (2 citations) 3–12% precast Bis-Tris gradient gels (2 citations)

2 protocols using nupage 4 12 bis tris gradient precast gels

Western Blot Analysis of Signaling Proteins

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Cells were washed with PBS twice, and lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Total protein lysates were run in Nupage 4–12% Bis-Tris gradient precast gels (Invitrogen) in MOPS buffer. Membranes were probed using specific antibodies: PTK2/FAK (#3285S; RRID: AB_2269034) pAKT (S473) (#4060; RRID: AB_2315049), pAKT (T308) (#13038; RRID: AB_2629447), total AKT (#4691; RRID: AB_915783), pS6 (S240/244) (#5364; RRID: AB_10694233), pS6 (S235/236) (#4858; RRID: AB_916156), HA-tag (#3724; RRID: AB_1549585), V5-tag (#13202; RRID: AB_2687461), β-actin (#4970S; RRID: AB_2223172) and vinculin (#13901; RRID: AB_2728768) were purchased from Cell Signaling Technology (CST). The same antibody was used to detect both PTK2/FAK (125 kDa) and FRNK (43 kDa). All primary antibodies were diluted 1:1000 and anti-rabbit IgG secondary antibody (GE Healthcare) (RRID: AB_772206) (1:10000) was used.

Ladewig E., Michelini F., Jhaveri K., Castel P., Carmona J., Fairchild L., Zuniga A.G., Arruabarrena-Aristorena A., Cocco E., Blawski R., Kittane S., Zhang Y., Sallaku M., Baldino L., Hristidis V., Chandarlapaty S., Abdel-Wahab O., Leslie C., Scaltriti M, & Toska E. (2022). The Oncogenic PI3K-Induced Transcriptomic Landscape Reveals Key Functions in Splicing and Gene Expression Regulation. Cancer Research, 82(12), 2269-2280.

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FOXA1 Protein Expression Western Blot

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Western blot was performed as previously described (Carracedo et al., 2012 (link)). Briefly, total protein lysates were run in Nupage 4–12% Bis-Tris gradient precast gels (Invitrogen) in MOPS buffer. Proteins were subjected to long migration time (~2.5h) in ice to allow correct separation and visualization of exogenous FOXA1-V5 and endogenous FOXA1. Primary antibodies used in this study are: rabbit anti-FOXA1 (CST, #58613), rabbit anti-V5 tag (D3H8Q, CST, # 13202) and rabbit anti-VINCULIN (CST, # 4650).

Arruabarrena-Aristorena A., Maag J.L., Kittane S., Cai Y., Karthaus W.R., Ladewig E., Park J., Kannan S., Ferrando L., Cocco E., Ho S.Y., Tan D.S., Sallaku M., Wu F., Acevedo B., Selenica P., Ross D.S., Witkin M., Sawyers C.L., Reis-Filho J.S., Verma C.S., Jauch R., Koche R., Baselga J., Razavi P., Toska E, & Scaltriti M. (2020). FOXA1 Mutations Reveal Distinct Chromatin Profiles and Influence Therapeutic Response in Breast Cancer. Cancer cell, 38(4), 534-550.e9.

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