Doxorubicin

Manufactured by Corning

Sourced in United States

Doxorubicin is a laboratory product used in research settings. It is a cytotoxic drug that can be used to study cell biology and drug development processes.

Automatically generated - may contain errors

Lab products found in correlation

4 hydroxytamoxifen, by Merck Group (1 mentions) Atplite, by PerkinElmer (1 mentions) Topotecan, by Merck Group (1 mentions) Hydroxyurea, by Selleck Chemicals (1 mentions) Cls3610, by Corning (1 mentions) 4 nitroquinoline, by Merck Group (1 mentions) Enspire plate reader, by PerkinElmer (1 mentions) Cell culture grade water, by Corning (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Safire2 plate reader, by Tecan (1 mentions) 96 well plate, by Corning (1 mentions) Doxorubicin, by Merck Group (1 mentions) Paclitaxel, by Merck Group (1 mentions) Docetaxel, by Merck Group (1 mentions) Epirubicin, by Corning (1 mentions) Epirubicin, by Merck Group (1 mentions) Celltiter 96 aqueous non radioactive cell proliferation assay solution, by Promega (1 mentions)

3 protocols using doxorubicin

Recql4 Knockout Cells Drug Sensitivity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hoxb8 immortalized [23 (link)] R26-CreER^T2Recql4^fl/+ (control) and R26-CreER^T2Recql4^fl/K525A cells were maintained in IMDM, 10% FBS (non-heat inactivated) and 1% GM-CSF containing media (BHK-HM5 cell conditioned media). The cells were treated for 4 days with 400nM 4-hydroxy tamoxifen (Merck Millipore) then genotyped to confirm complete recombination. Cells were then plated at 10,000 cells/well in 96 well plates (Corning, CLS3610) and incubated for 48 hours with the indicated concentration of drugs in triplicates per dose (dose range Doxorubicin: 0–0.5μM, Hydroxyurea: 0–0.5mM, 4-Nitroquinoline: 0–2μM and Topotecan: 0–0.5mM). Doxorubicin was obtained from St. Vincent’s Hospital Pharmacy. Hydroxyurea was purchased from Selleck. 4-Nitroquinoline and Topotecan were purchased from Sigma-Aldrich. Cell viability was measured using ATP-Lite (Perkin Elmer) as directed by the manufacturer and measured on an EnSpire plate reader (Perkin Elmer). Data were plotted and the IC₅₀ value calculated using Prism 7 software. The dose-response curve was plotted as mean±SEM.

Castillo-Tandazo W., Smeets M.F., Murphy V., Liu R., Hodson C., Heierhorst J., Deans A.J, & Walkley C.R. (2019). ATP-dependent helicase activity is dispensable for the physiological functions of Recql4. PLoS Genetics, 15(7), e1008266.

+ Open protocol

+ Expand

Cardiotoxicity Evaluation in hiPSC-CMs

Check if the same lab product or an alternative is used in the 5 most similar protocols

doxorubicin hydrochloride (MedChem Express) was resuspended to 10 mM in cell culture-grade water (Corning) and aliquots were stored at −20 °C. Day 30 hiPSC-CMs were treated for 24 h or 72 h with doxorubicin (0.01-100 μM) diluted in RPMI 1640 medium (no phenol red, Corning) supplemented with 500 μg/ml recombinant human serum albumin (Oryzogen). For RARG agonist treatment, day 30 hiPSC-CMs were treated with respective agonists (all from Tocris, resuspended in DMSO) for 24 h prior to doxorubicin administration and then a second dose was co-administered with doxorubicin as above.

Magdy T., Jiang Z., Jouni M., Fonoudi H., Lyra-Leite D., Jung G., Romero-Tejeda M., Kuo H.H., Fetterman K.A., Gharib M., Burmeister B.T., Zhao M., Sapkota Y., Ross C.J., Carleton B.C., Bernstein D, & Burridge P.W. (2021). RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy. Cell stem cell.

+ Open protocol

+ Expand

Cytotoxicity of Chemotherapeutic Agents

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Epirubicin, doxorubicin, paclitaxel and docetaxel were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Drugs were dissolved in dimethyl sulfoxide and aliquots of stock solutions were frozen at −80 °C. Cell proliferation assays were performed in triplicate at each drug concentration. Cytotoxicity assays with the lymphoblastoid and tumor cell lines were performed in triplicate at each dose. Specifically, 90 μl of cells (5 × 10³ cells per ml) were plated into 96-well plates (Corning, Corning, NY, USA)^{37 (link)} and were treated with 10 μl of Epirubicin or doxorubicin at final concentrations of 0, 0.0156, 0.03125, 0.0625, 0.125, 0.25, 0.55, 1 and 2 μmol l⁻¹. Similarly, cells were treated with paclitaxel or docetaxel at 0, 0.01, 0.1, 1, 10, 50, 100, 1000 and 5000 nmol l⁻¹. After incubation for 72 h, 20 μl of CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay solution (Promega Corporation) was added to each well. Plates were read in a Safire2 plate reader (Tecan AG).

Hanson C., Cairns J., Wang L, & Sinha S. (2015). Computational discovery of transcription factors associated with drug response. The Pharmacogenomics Journal, 16(6), 573-582.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!