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Zinc sulfate monohydrate

Manufactured by Merck Group
Sourced in United States, Germany

Zinc sulfate monohydrate is a chemical compound with the formula ZnSO4·H2O. It is a white, crystalline solid that is commonly used in various laboratory and industrial applications. The compound is soluble in water and has a wide range of uses, including as a nutrient supplement, a reagent in chemical reactions, and a source of zinc ions in various applications.

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6 protocols using zinc sulfate monohydrate

1

Synthesis of Metal Nanoparticles

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Hexane (anhydrous, 95%), ethyl alcohol (EtOH),
oleic acid (OA, 99%), tri-n-octylamine (TOA, >98.0%),
and sodium oleate (NaOL, >97.0%) were purchased from Alfa Aesar.
Sodium
hydroxide (NaOH), iron(III) chloride hexahydrate (FeCl3·6H2O, 97.0–102.0%), zinc sulfate monohydrate
(ZnSO4·H2O), manganese chloride tetrahydrate
(MnCl2·4H2O), sodium sulfide (Na2S), and l-cysteine were purchased from Sigma-Aldrich and
were used without further purification.
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2

Intranasal Zinc Sulfate and Anesthetic Injection

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Zinc sulfate monohydrate (Sigma, USA) was dissolved in a sterile 0.9% sodium chloride solution to a final concentration of 5% and was sprayed intranasal as 0.1 cc solution with 0.4 cc air. Ketamine and Xylazine were diluted to a final concentration of 75 mg/ml and 5 mg/ml in a sterile 0.9% sodium chloride solution and injected intramuscular at 75 mg/kg and 5 mg/kg, respectively.
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3

Protein Stabilization and Characterization

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BSA [molecular weight (MW), ~66.5 kDa) was purchased from Rocky Mountain Biologicals. Sodium phosphate monobasic anhydrous (NaHPO4) (MW, 119.98 g/mol) was obtained from Research Products International. Sodium chloride (NaCl) (MW, 58.44 g/mol) was purchased from Thermo Fisher Scientific. Ammonium persulfate (APS) [(NH4)2S2O8] (MW, 228.20 g/mol; 1 M) solution, tris(bipyridine) ruthenium(II) chloride {[Ru(bpy)3]2+} (MW, 748.62 g/mol; 6.67 mM) solution, Trizma base NH2C(CH2OH)3 (MW, 121.14 g/mol), hydrochloric acid (HCl) (37%), zinc sulfate monohydrate (ZnSO4·H2O; purity, ≥99.9%) (MW, 179.47 g/mol), copper(ii) chloride dihydrate (CuCl2·2H2O; purity, ≥99.99%) (MW, 170.48 g/mol), and Sigmacote were purchased from Sigma-Aldrich. Tris [20 mM Tris-NaCl and 150 mM NaCl (pH ~7.4)] and phosphate-buffered saline [10 mM NaHPO4 and 150 mM NaCl (pH ~3)] were used as buffers. Double distilled H2O was used in all solution preparations.
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4

Zinc Sulfate Supplementation in Calves

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All calves were under the same management practices including housing and diet14 (link). Throughout the study, calves were housed in individual metal hutches. The respective treatments were administered in the morning milk bottles for 14 days starting from enrollment; calves in the zinc sulfate treatment group received 0.22 g zinc sulfate monohydrate (80 mg of elemental zinc) (Sigma-Aldrich Company, St. Louis, MO, United States) with 0.44 g milk replacer powder, while those in the placebo group received only milk replacer powder (0.44 g). Antimicrobials included in dietary milk for calves 0–25 days of age included chlortetracycline hydrochloride (Pennchlor 64, Pharmgate Animal Health, Omaha, NE, United States) and neomycin sulfate (NeoMed 325, Bimeda, Inc., Le Sueur, MN, United States). Calves 25 days of age to weaning received dietary milk that included oxytetracycline hydrochloride (NT-10G, Agri-Best™, Strauss Feeds LLC, Watertown, WI, United States). Calves from which samples were employed for the current study were all treated for symptoms of diarrhea at some point over the duration of the trial and received the same treatment consisting of an oral mixture of 118.5 mL bismuth subsalicylate (Bismusal Suspension, Durvet, Inc., Blue Springs, MO, United States) and 31.5 mL of spectinomycin (SpectoGard, Bimeda, Inc., Le Sueur, MN, United States)14 (link).
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5

Removal of Methylmercury from Water

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MeHgCl, tiourea, 2-mercaptobenzimidazole (MBI), 2-mercaptobenzothiazole (MBT), acrylic acid (AA), ethylene glycol dimethacrylate (EGDMA) and azodiisobutyronitrile (AIBN) were purchased from Sigma-Aldrich (St. Louis, MO, USA); ethanol (EtOH) absolute (99.9%) was obtained from JT Baker (Radnor, PA, USA); MeHgCl standard solution 1000 mg L−1 from Alfa Aesar (Tewksbury, MA, USA); and hydrochloric acid (HCl), sodium acetate (NaCH3COO), acetic acid (CH3COOH), sodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), sodium hydroxide (NaOH), and sodium tetraborate (Na2B4O7) were purchased from Merck (Darmstadt, Germany);. For the selectivity study, solutions were prepared from salt standards of lead nitrate, cadmium nitrate tetrahydrate, zinc sulfate monohydrate, and mercury dichloride (all acquired form Sigma-Aldrich). All the reagents used in this work were of analytical grade, and the solutions were prepared in ultrapure water (resistivity of 18.2 MΩ cm).
The samples were collected from the Batalha river (21.72 S, 49.20 W), and directly from the tap.
WARNING: MeHgCl appears as white microcrystals and is very toxic by inhalation, in contact with skin and if swallowed. Protection measures such as glasses, full face-shields, gloves and particulate respirators are required to manipulate it. Its disposal should also be carried out carefully.
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6

Characterization of Insulin-Protamine Complex

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Insulin human recombinant, expressed in yeast ([?]98% purity, MW 5.8 kDa ), protamine sulfate salt from salmon (MPRRRRSSSRPVRRRRRPRVSRRRRRRGGRRRR) ( MW 5.1 kDa), 8 anilino 1 naphthalenesulfonic acid ammonium salt (ANS) and zinc sulfate monohydrate (ZnSO 4 ) were purchased from Sigma Aldrich. Na 2 HPO 4 , NaH 2 PO 4 , NaCl and phenol were purchased from Merck-chemicals Ltd. All experiments were performed in phosphate buffer at pH 8.0, containing 10 mM Na 2 HPO 4 , 10 mM NaH 2 PO 4 , and 100 mM NaCl. The concentration of insulin was measured using absorbance a 276 nm on the Cary-100 UV-Vis spectrophotometer. The molar extinction coefficient used for insulin was 6200 M -1 cm -19 .
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