Chromanorm

Manufactured by Avantor

Sourced in United States

Chromanorm is a line of laboratory equipment designed for chromatography applications. The core function of Chromanorm products is to facilitate the separation, identification, and purification of chemical compounds in a laboratory setting.

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HiPerSolv CHROMANORM for LC–MS Acetonitrile HiPerSolv CHROMANORM LC-MS grade HiPerSolv CHROMANORM gradient grade HiPerSolv CHROMANORM for HPLC Water HiPerSolv Chromanorm Methanol HiPerSolv CHROMANORM LC-MS grade HiPerSolv CHROMANORM quality Acetonitrile HiPerSolv Chromanorm HiPerSolv Chromanorm

9 protocols using chromanorm

Ni Foil Anodization Experiments

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For the anodization experiments
Ni foils
(99.9+%, 0.125 mm thick), NH₄F (≥99%), and ethylene
glycol (≥99%) were all purchased from Sigma-Aldrich, and KOH
(≥86% in water) was obtained from Fluka. For cleaning the metal
foils ethanol (Chromanorm) 2-propanol (Chromanorm), and acetone (Rectapur)
were purchased from VWR. For the electrochemical measurements Na₂SO₄ (99%, Alfa Aesar) was used, along with N₂ gas (99.995%, Messer, Budapest, Hungary). All solutions were
prepared with ultrapure water (Milli-Q, ρ = 18.2 MΩ cm).
All chemical reagents were used without further purification.

Sápi A., Varga A., Samu G.F., Dobó D., Juhász K.L., Takács B., Varga E., Kukovecz Á., Kónya Z, & Janáky C. (2017). Photoelectrochemistry by Design: Tailoring the Nanoscale Structure of Pt/NiO Composites Leads to Enhanced Photoelectrochemical Hydrogen Evolution Performance. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 121(22), 12148-12158.

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Quantitative HPLC-MS/MS Analysis of Ribonucleosides

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Ribonucleosides were separated using a Luna Omega Polar (150 × 2.1 mm; particle size, 2.5 μm; pore size, 100 Å; Phenomenex, Torrance, CA) on an Agilent 1290 series HPLC system equipped with a diode array detector. Mobile phase A was 5 mM NH₄OAc (≥99%; HiPerSolv CHROMANORM, VWR) adjusted to pH 5.3 with glacial acetic acid (≥99%, HiPerSolv CHROMANORM, VWR), and mobile phase B was pure acetonitrile (LC-MS grade, purity ≥99.95; Roth). Gradient elution started with 98% A and increased to 10% B after 2 min, 30% B after 3 min, and 60% B after 3.5 min. Starting conditions are re-established at 4 min followed by 2 min of equilibration. The flow rate was 0.4 ml/min, and the column temperature was 30°C. The effluent from the column was directed through the DAD (diode array detector) before entering the Agilent 6490 Triple Quadrupole mass spectrometer in dynamic multiple reaction monitoring (MRM) mode. The MS was operated in positive-ion mode with the following parameters: electrospray ionization (ESI-MS, Agilent Jetstream); fragmentor voltage, (set in tunefile to) 250 V; cell accelerator voltage, 2 V; N₂-gas temperature, 150°C; N₂-gas flow, 15 liters/min; nebulizer, 30 psi; sheath gas (N₂) temperature, 275°C; sheath gas flow, 11 liters/min; capillary, 2500 V; and nozzle voltage, 500 V. The instrument was operated in dynamic MRM mode with the method listed in table S2.

Ignatova V.V., Kaiser S., Ho J.S., Bing X., Stolz P., Tan Y.X., Lee C.L., Gay F.P., Lastres P.R., Gerlini R., Rathkolb B., Aguilar-Pimentel A., Sanz-Moreno A., Klein-Rodewald T., Calzada-Wack J., Ibragimov E., Valenta M., Lukauskas S., Pavesi A., Marschall S., Leuchtenberger S., Fuchs H., Gailus-Durner V., de Angelis M.H., Bultmann S., Rando O.J., Guccione E., Kellner S.M, & Schneider R. (2020). METTL6 is a tRNA m3C methyltransferase that regulates pluripotency and tumor cell growth. Science Advances, 6(35), eaaz4551.

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HPTLC Analysis of Sildenafil and Tadalafil

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Reagents and analytical standards used for the implementation of the HPTLC method were as follows: methanol (HiPerSolv purity ≥ 99,8%), dichloromethane (HiPerSolv purity ≥ 99,8%), chloroform (HiPerSolv CHROMANORM, purity ≥ 99,8%), acetone (HiPerSolv CHROMANORM) all from VWR; ammonium hydroxide solution (25%) from Honeywell Fluka™); and diethylamine (purity ≥ 99.5%) from Sigma-Aldrich.
Sildenafil citrate (purity ≥ 98%) was from Sigma-Aldrich and Viagra® tablets 50 mg B/4 B226423B from Pfizer limited. ERECTAD® (tadalafil tablets) 20 mg B/4 AT2E-1601 was from ASMOH Laboratories Limited.

Sakira A.K., Ouattara M.S., Yabre M., Bande M, & Some T.I. (2021). Contribution to the Detection of Poor Quality Sildenafil Drugs in Burkina Faso Using High-Performance Thin-Layer Chromatography. Journal of Analytical Methods in Chemistry, 2021, 4093859.

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Magnetite Nanoparticle-Based Solid-Phase Extraction

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All chemical used during method development were of analytical grade, unless stated otherwise. Caffeine was purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO; United States). Stock solutions (100 mg L⁻¹) were prepared in methanol and stored at 4 °C. Working solutions were prepared daily by diluting appropriate quantity of stock standard solution with ultrapure water. Acetonitrile (≥99.9%) CHROMANORM® of LC-MS grade was purchased from VWR International (VWR International, Radnor, Pennsylvania, United States).
For the synthesis of the magnetite nanoparticles (Fe₃O₄), iron(iii) chloride hexahydrate (FeCl₃·6H₂O), iron(ii) chloride tetrahydrate (FeCl₂·4H₂O) and ammonia solution (NH₃) were purchased from Sigma-Aldrich. All chemicals used during the synthesis of the sorbent were of reagent grade. Wood-based activated carbon (BAX-1500) was manufactured by Mead Westvaco (Richmond, Virginia, USA). River and lake surface water samples were collected in Vienna (Austria) and filtered with nylon filter membranes, pore size 0.45 μm, prior to the MSPE procedure.

Manousi N., Deliyanni E.A., Rosenberg E, & Zachariadis G.A. (2021). Magnetic solid-phase extraction of caffeine from surface water samples with a micro–meso porous activated carbon/Fe3O4 nanocomposite prior to its determination by GC-MS. RSC Advances, 11(32), 19492-19499.

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Solvent Preparation for Analytical Methods

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Acetonitrile (ACN) and methanol (MeOH) (Chromanorm) and the formic acid Emparta (FA) were purchased from VWR (Milan, Italy). Ultrapure water was obtained via a Milli-Q apparatus by the Millipore corporation (Burlington, MA, USA).

Clericuzio M., Novello G., Bivona M., Gamalero E., Bona E., Caramaschi A., Massa N., Asteggiano A, & Medana C. (2024). A Study of Metabolites from Basidiomycota and Their Activities against Pseudomonas aeruginosa. Antibiotics, 13(4), 326.

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Analytical Extraction and Fractionation

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Solvents for extraction and fractionation (analytical grade) as well as HPLC-grade acetonitrile (Chromanorm) and methanol (LiChrosolv) were obtained from VWR International (West Chester, Pennsylvania, USA). Glacial acetic acid (Rotichrom) was purchased from Carl Roth (Karlsruhe, Germany). Genistein (HPLC quality) for the dereplication approach was purchased from Sigma-Aldrich (St. Louis, Missouri, USA).

Ateba S.B., Njamen D., Medjakovic S., Zehl M., Kaehlig H., Jungbauer A, & Krenn L. (2014). Lupinalbin A as the most potent estrogen receptor α- and aryl hydrocarbon receptor agonist in Eriosema laurentii de Wild. (Leguminosae). BMC Complementary and Alternative Medicine, 14, 294.

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Preparation of Analytical-Grade Solvents

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All solvents were analytical-grade or HPLC-grade. ACN (Chromanorm), EtOAc (Normapur) and MeOH (LiChrosolv) were obtained from VWR International (Radnor, Pennsylvania, PA, USA). Toluene and ethylformate were acquired from Merck KGaA (Darmstadt, Germany). HCOOH was obtained from Carl Roth GmbH (Karlsruhe, Germany).

Mittermair E., Kählig H., Tahir A., Rindler S., Hudec X., Schueffl H., Heffeter P., Marian B, & Krenn L. (2020). Methylated Xanthones from the Rootlets of Metaxya rostrata Display Cytotoxic Activity in Colorectal Cancer Cells. Molecules, 25(19), 4449.

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Synthesis and Characterization of Nucleoside Analogues

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1-Methyl-2(1H)-pyrimidinone (Specs), β-D-ribose (97%, Sigma-Aldrich) and zebularine (≥98%, Sigma-Aldrich) were used as received. The solvents water (Chromanorm, VWR chemicals), methanol (Uvasol, Merck KGaA), perdeuterated methanol (Euriso-top, 99.8%), and tetrahydrofuran (Uvasol, Merck KGaA) were of spectroscopic grade. Research grade gases (nitrogen (N50), oxygen (N48), and nitrous oxide (N25)) were supplied by Air Liquide.

Micheel M., Torres Ziegenbein C., Gilch P, & Ryseck G. (2015). Pyrimidinone: versatile Trojan horse in DNA photodamage?. Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 14(9).

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Optimized Purification Procedures

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All chemicals used were reagent grade and used as supplied except where noted (Sigma Aldrich, TCI, Carl Roth, VWR, etc.). Solvents for chromatography and workup procedures were obtained from VWR (Chromanorm, HPLC grade). Reactions were performed under an argon atmosphere except where noted. Analytical thin-layer chromatography was performed on E. Merck silica gel 60 F 254 plates (0.25 mm). Compounds were visualized by UV-light at 254 nm and by dipping the plates in ninhydrin solution, cerium sulfate ammonium molybdate (CAM) solution, or permanganate stain. Liquid chromatography was performed using forced flow of the indicated solvent on Normasil 60 silica gel 40-63 mm (VWR, Dresden).

Klapper M., Braga D., Lackner G., Herbst R, & Stallforth P. (2018). Bacterial Alkaloid Biosynthesis: Structural Diversity via a Minimalistic Nonribosomal Peptide Synthetase. Cell chemical biology, 25(6).

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