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Impress excel polymer system

Manufactured by Vector Laboratories
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The ImPRESS™ Excel Polymer system is a lab equipment product offered by Vector Laboratories. It is a detection system designed for use in immunohistochemistry and in situ hybridization applications.

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Lab products found in correlation

Dibutylphthalate polystyrene xylene (dpx), by Merck Group (3 mentions) Streptavidin biotin horseradish peroxidase complex, by Agilent Technologies (2 mentions) 3 3 diaminobenzidine (dab), by Merck Group (2 mentions)

3 protocols using impress excel polymer system

1

Immunohistochemistry Protocol for Tissue Sections

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Five micrometer formalin-fixed tissue sections were dewaxed in xylene, then rehydrated through ethanol into water. Antibodies are detailed in Table S3. Following appropriate antigen retrieval (Table S3), sections were incubated with optimally titrated primary antibodies and immunodetectedusing secondary antibodies linked to streptavidin-biotin horseradish peroxidase complex (DAKO), ImPRESS™ Excel Polymer system (Vector labs), or BenchMark Ultra automated staining system (Ventana) (Table S3). All slides were counterstained in Mayer's haematoxylin and mounted in DPX (Sigma). Control slides and tissues were included to check specificity.
Daza-Cajigal V., Albuquerque A.S., Pearson J., Hinley J., Mason A.S., Stahlschmidt J., Thrasher A.J., Mishra V., Southgate J, & Burns S.O. (2019). Loss of Janus Associated Kinase 1 Alters Urothelial Cell Function and Facilitates the Development of Bladder Cancer. Frontiers in Immunology, 10, 2065.
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2

Immunohistochemical Analysis of Tight Junctions in Human Ureter

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Sections (5 μM) of human ureter were dewaxed and rehydrated. For immunolabelling with ZO-1 and claudin 3 antibodies, endogenous avidin and biotin were blocked and antigen retrieval was performed by boiling sections for 10 min in 10 mM citric acid buffer, pH 6.0. After a 16 h incubation of primary antibody at 4°C, slides were washed, incubated in biotinylated secondary antibody and visualised by addition of streptavidin-biotin horseradish peroxidase complex (DAKO) and 3,3′-diaminobenzidine (Sigma Aldrich). For ZO-1α+ labelling, antigen retrieval was performed by boiling of slides in 1 mM EDTA in 10 mM Tris-HCl buffer (pH 9.0) before incubating with primary antibody for 16 h at 4°C. Antibody binding was visualised using the ImPRESS™ Excel Polymer system (Vector labs), according to the manufacturer's instructions. All slides were counterstained in Mayer's haematoxylin and mounted in DPX (Sigma).
Smith N.J., Hinley J., Varley C.L., Eardley I., Trejdosiewicz L.K, & Southgate J. (2015). The human urothelial tight junction: claudin 3 and the ZO-1α+ switch. Bladder, 2(1), e9-.
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3

Immunohistochemical Analysis of Tight Junctions in Human Ureter

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Sections (5 μM) of human ureter were dewaxed and rehydrated. For immunolabelling with ZO-1 and claudin 3 antibodies, endogenous avidin and biotin were blocked and antigen retrieval was performed by boiling sections for 10 min in 10 mM citric acid buffer, pH 6.0. After a 16 h incubation of primary antibody at 4°C, slides were washed, incubated in biotinylated secondary antibody and visualised by addition of streptavidin-biotin horseradish peroxidase complex (DAKO) and 3,3′-diaminobenzidine (Sigma Aldrich). For ZO-1α+ labelling, antigen retrieval was performed by boiling of slides in 1 mM EDTA in 10 mM Tris-HCl buffer (pH 9.0) before incubating with primary antibody for 16 h at 4°C. Antibody binding was visualised using the ImPRESS™ Excel Polymer system (Vector labs), according to the manufacturer's instructions. All slides were counterstained in Mayer's haematoxylin and mounted in DPX (Sigma).
Smith N.J., Hinley J., Varley C.L., Eardley I., Trejdosiewicz L.K, & Southgate J. (2015). The human urothelial tight junction: claudin 3 and the ZO-1α+ switch. Bladder, 2(1), e9-.
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