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1m tris hcl

Manufactured by Solarbio
Sourced in China

1M Tris-HCl is a buffer solution commonly used in various laboratory applications. It is a concentrated form of Tris (tris(hydroxymethyl)aminomethane) hydrochloride, which is a widely used buffer system for maintaining pH in biological and biochemical experiments.

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2 protocols using 1m tris hcl

1

Chemiluminescent Detection of Mycotoxins

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N, N-dimethylformamide (DMF) was purchased from Aladdin Chemical Co. Ltd. (Shanghai, China). Hemin, luminol, 20× phosphate-buffered saline (pH 7.4), 1M Tris-HCL (pH 7.4) and 1M Tris-HCL (pH 9.0) were purchased from Solarbio Life Sciences (Beijing, China). The 1 mM Hemin and 30 mM luminol were prepared in DMF and stocked in the dark at 4 °C. H2O2 (30%), methanol (HPLC grade), magnesium chloride hexahydrate and potassium chloride were obtained from Beijing Jingri Jindian Technology Co., Ltd. (Beijing, China). Zearalenone (ZEN), aflatoxin B1 (AFB1), deoxynivalenol (DON) and ochratoxin A (OTA) were ordered from Meizheng Bio-Tech (Beijing, China). Costar 96-well white flat-bottom microplates were purchased from Corning (Beijing, China). All the oligonucleotides were synthesized by Tsingke Biotechnology (Beijing, China) and prepared as stocks of 10 μM in nuclease-free water.
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2

Peptide-Based Biosensor Fabrication

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Urea, sodium chloride (NaCl), potassium ferricyanide (K3Fe(CN)6) were obtained from Sinopharm Chemical Reagent Company (Shanghai, China). 1 M Tris–HCl, Bovine Serum Albumin (BSA) were supplied by Solarbio Company (Beijing, China). PCT, C-reaction protein and Interleukin-6 were purchased from Cusag Company (Wuhan, China). The capturing peptide powder was synthesized from Top-peptide Company (Shanghai, China), and the sequence of capturing peptide was displayed as follows:34 (link)PP: TSCNGHTCTRPNF.
All experimental water was ultrapure water by Mill-Q water purification (18.2 MΩ).
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