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Chiba needle

Manufactured by Cook Medical
Sourced in United States

The Chiba needle is a medical device used for various diagnostic and therapeutic procedures. It is a type of fine-bore needle designed for percutaneous interventions. The Chiba needle is typically used for procedures such as biopsies, aspirations, and other image-guided interventions.

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4 protocols using chiba needle

1

Pericardial Drainage Using Ultrasound-Guided Technique

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All PCD procedures were performed by one interventional radiologist with 20 years of experience. After administration of 1% lidocaine to the skin and the deeper tissue of the subxiphoid area, a 21-gauge Chiba needle (Cook, Bloomington, IN, USA) was advanced via a subxiphoid approach under ultrasound and fluoroscopic guidance (Fig 1A). The Chiba needle was advanced into the skin at a cephalad angle, and the coronary, pericardial, and internal mammary arteries were avoided. Once the fluid-filled pericardial space was entered with the Chiba needle tip under ultrasound guidance, a 0.018-inch guidewire (Cook) was introduced into the pericardial space through the Chiba needle under fluoroscopic guidance (Fig 1B).
Then, the Chiba needle was exchanged for a 6-Fr Neff catheter (Cook). A 0.035-inch stiff guidewire (Terumo, Tokyo, Japan) was advanced through the Neff catheter (Fig 1B), and an 8.5-Fr pigtail catheter (Cook) was inserted into the pericardial space over the 0.035-inch guidewire (Fig 1C). After the effusion was manually drained, the position of the catheter was confirmed to be in the pericardial space by injection of a contrast agent (Fig 1D). The catheter was secured to the skin with 2–0 silk sutures and maintained in place until the amount of drainage was less than 30 mL/day [3 ].
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2

Isolating and Cryopreserving Immune Cells

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PBMCs were purified from whole blood or leukapheresis products via standard density gradient centrifugation and cryopreserved at −140°C. Lymph node mononuclear cells were isolated via mechanical disruption and cryopreserved at −140°C. Non-lymphoid mononuclear cells were isolated using a combination of mechanical disruption and collagenase treatment. TDL was accessed as described previously (Nadolski and Itkin, 2012 (link)). Briefly, a 25-gauge spinal needle was inserted under ultrasound guidance into an inguinal LN on each side of the body, and the oil-based contrast agent ethiodol (Savage Laboratories) was injected under fluoroscopic guidance into each LN. After opacification of the cisterna chyli, access was gained via an anterior transabdominal approach using a 21-gauge or a 22-gauge Chiba needle (Cook Medical Inc.), and a V-18 control guidewire (Boston Scientific) was inserted into the thoracic duct and manipulated cephalad, followed by a 60-cm 2.3F Rapid Transit Microcatheter (Cordis Corp.), which was advanced further into the thoracic duct to aspirate TDL. Aspirated TDL was collected in heparin tubes, and the contrast agent was removed via standard density gradient centrifugation. TDL samples were used directly in flow cytometry experiments or cryopreserved at −140°C.
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3

Percutaneous I-125 Seed Implantation

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The operation was performed under local anesthesia by experienced interventional radiologists with more than 20 years of experience. A Chiba needle (Cook Medical, Bloomington, IN, USA) was used to puncture the second-order portal vein with color Doppler ultrasound guidance. A 6-Fr outer sleeve (Cook, Inc.) was inserted into the portal vein, followed by a 5-Fr sheath (Cordis, Miami Lakes, FL, USA). The I-125 seed strand was delivered to the target position through the 5-Fr sheath. The puncture tract then was occluded by coils or gelatin sponge (Figure 1).
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4

Isolating and Cryopreserving Immune Cells

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PBMCs were purified from whole blood or leukapheresis products via standard density gradient centrifugation and cryopreserved at -140°C. Lymph node mononuclear cells were isolated via mechanical disruption and cryopreserved at -140°C. Non-lymphoid mononuclear cells were isolated using a combination of mechanical disruption and collagenase treatment. TDL was accessed as described previously (Nadolski and Itkin, 2012) . Briefly, a 25-gauge spinal needle was inserted under ultrasound guidance into an inguinal LN on each side of the body, and the oil-based contrast agent ethiodol (Savage Laboratories) was injected under fluoroscopic guidance into each LN. After opacification of the cisterna chyli, access was gained via an anterior transabdominal approach using a 21-gauge or a 22-gauge Chiba needle (Cook Medical Inc.), and a V-18 control guidewire (Boston Scientific) was inserted into the thoracic duct and manipulated cephalad, followed by a 60-cm 2.3F Rapid Transit Microcatheter (Cordis Corp.), which was advanced further into the thoracic duct to aspirate TDL. Aspirated TDL was collected in heparin tubes, and the contrast agent was removed via standard density gradient centrifugation. TDL samples were used directly in flow cytometry experiments or cryopreserved at -140°C.
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