Avicel® PH 101 (Sigma Aldrich, St. Louis, USA) and milled birch wood (Betula pubescens) SE at 210 °C for 10 min [21 (link)] were used as substrates. Additionally, a sample of SE birch was extensively washed by six cycles of resuspending in Milli-Q water followed by centrifugation at 8000 rpm for 15 min, in order to prepare a substrate without the soluble compounds generated during pretreatment. Phosphoric acid swollen cellulose (PASC), used for characterization of LPMO activity, was prepared from Avicel using the method described by Wood [30 ].
Ph 101
Manufactured by Merck Group
Sourced in United States
The PH 101 is a laboratory pH meter designed for basic pH measurement and analysis. It features a digital display, automatic temperature compensation, and a standardized electrode connection. The PH 101 provides accurate and reliable pH readings for routine laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ph 101
1
Substrate Preparation for LPMO Analysis
2
Cultivation of E. coli and C. thermocellum
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The strains used in this study are listed in table S1. E. coli strains were routinely cultivated in Luria-Bertani (LB) medium with shaking at 200 rpm at 37°C. C. thermocellum strains were grown anaerobically at 55°C in GS-2 medium (47 (link)) with fructose (5 g/liter), glucose (5 g/liter), cellobiose (5 g/liter), or Avicel (5 g/liter; PH-101, Sigma-Aldrich) as the sole carbon source. When required, the media for E. coli and C. thermocellum were supplemented with kanamycin (50 μg/ml), chloramphenicol (30 μg/ml), or thiamphenicol (Tm; 3 μg/ml).
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