Agarose, low electroendosmosis was purchased from Alfa Aesar (Ward Hill, MA, USA). Av, FITC conjugate, Av, Texas Red conjugate, NeutrAvidin (Nu), FITC conjugate, Nu, Texas Red conjugate, Calcein AM, and Ethidium Homodimer-1 were purchased from Invitrogen (Eugene, OR, USA). Protease Inhibitor Mini Tablets, EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride), Sulfo-NHS (N-hydroxysulfosuccinimide), Quant-iT PicoGreen dsDNA Assay Kit, and Dimethyl Sulfoxide (DMSO) were purchased from Thermo Scientific Pierce (Rockford, IL, USA). Trypsin–EDTA phenol red, high glucose DMEM, and Pen-Strep Antibiotic–Antimycotic were purchased from Gibco (Carlsbad, CA, USA). Proteinase-K was purchased from Roche Diagnostics (Risch-Rotkreuz, Switzerland). Nonfat-dried bovine milk, Fluorescein isothiocyanate-Dextran (FITC-Dextran), Fluorescein isothiocyanate isomer 1, Bovine Serum Albumin, 4-(Dimethylamino)pyridine (DMAP), Pyridine, Succinic Anhydride (SA), 2-(N-Morpholino)ethanesulfonic acid (MES), Dimethylmethylene Blue (DMMB), and other salts and reagents were purchased from Sigma (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Atlanta Biologics. Nitric oxide (NO) Griess Reagent System was purchased from Promega (Madison, WI, USA).
Trypsin edta phenol red
Manufactured by Thermo Fisher Scientific
Sourced in United States
Trypsin-EDTA phenol red is a cell dissociation reagent used to detach adherent cells from culture surfaces. It contains the proteolytic enzyme trypsin and the chelating agent EDTA, which helps to break down the extracellular matrix and cell-cell adhesions. The phenol red indicator is included for pH monitoring purposes.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor mini tablet, by Thermo Fisher Scientific (3 mentions)
Proteinase k, by Roche (3 mentions)
K alpha, by Thermo Fisher Scientific (2 mentions)
Fluorescein isothiocyanate dextran, by Merck Group (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Fitc conjugate, by Thermo Fisher Scientific (1 mentions)
Texas red conjugate, by Thermo Fisher Scientific (1 mentions)
Fluorescein isothiocyanate isomer 1, by Merck Group (1 mentions)
Succinic anhydride sa, by Merck Group (1 mentions)
Ethidium homodimer 1, by Thermo Fisher Scientific (1 mentions)
Calcein am, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Fluorescein diacetate, by Merck Group (1 mentions)
Chondroitinase abc, by Merck Group (1 mentions)
Tris hcl buffer, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Thermo Fisher Scientific (1 mentions)
L proline, by Thermo Fisher Scientific (1 mentions)
Recombinant human il 1α, by Thermo Fisher Scientific (1 mentions)
Recombinant human igf 1, by Thermo Fisher Scientific (1 mentions)
4 protocols using trypsin edta phenol red
1
Multifunctional Biomolecular Labeling Protocol
2
Chondrocyte Differentiation Induction Protocol
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Human recombinant IL-1α and human recombinant IGF-1 were purchased from PeproTech (Rocky Hill, NJ). Dulbecco’s modification of Eagle’s medium (DMEM) was purchased from Corning (Corning, NY). Trypsin-EDTA phenol red, HEPES buffer, non-essential amino acids (NEAA), and penicillin streptomycin antibiotic-antimycotic (PSA) were purchased from Gibco (Carlsbad, CA). Ascorbic acid (AA) and L-proline were from Fisher Bioreagents (Pittsburgh, PA). Proteinase-K was purchased from Roche Diagnostics (Risch-Rotkreuz, Switzerland). NHS-PEG2-Maleimide and Protease Inhibitor Mini Tablets were from Thermo Scientific Pierce (Rockford, IL). Propidium iodide (PI) was obtained from Thermofisher Acros Organics (Geel, Belgium). Tris-HCl buffer was purchased from Invitrogen (Grand Island, NY). Chondroitinase ABC, Resazurin sodium salt, Griess reagent, fluorescein diacetate (FDA), and other salts and reagents were purchased from Sigma (St. Louis, MO).
3
Protease Inhibitor Mini Tablet Preparation
4
Comprehensive Surface Analysis of Osteoblast Cultures
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Surface topography was examined under scanning electron microscopy (SEM; S-3400N, Hitachi High-Technologies, Tokyo, Japan) and surface roughness (Ra, the arithmetic average of the roughness profile) was measured under laser scanning microscopy (VK-9710, Keyence, Osaka, Japan) at each time point. The elemental composition of each D0 and D56 surface was examined using X-ray photoelectron spectroscopy (XPS; K-Alpha, Thermo Fisher Scientific, Waltham, MA, USA). The surface wettability of D0 and D56 disks in each surface group was also examined by using the sessile drop technique to measure the water contact angle (DM 500FK, Drop Master, Kyowa Interface Science, Saitama, Japan).
Cell culture MC3T3-E1 cells from an osteoblast precursor cell line derived from murine calvaria were used in this study. These cells were cultured with alpha-minimum essential medium (α-MEM; Gibco, Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco). At confluence, the cells were detached using 0.05% trypsin-EDTA phenol red (Gibco) and reseeded for further experiments.
Cell culture MC3T3-E1 cells from an osteoblast precursor cell line derived from murine calvaria were used in this study. These cells were cultured with alpha-minimum essential medium (α-MEM; Gibco, Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco). At confluence, the cells were detached using 0.05% trypsin-EDTA phenol red (Gibco) and reseeded for further experiments.
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