89Zr was produced at Memorial Sloan Kettering Cancer Center using an EBCO TR19/9 variable-beam energy cyclotron (Ebco Industries Inc., British Columbia, Canada) via the 89Y(p,n)89Zr reaction. 89Zr was purified in accordance with previously reported methods to create 89Zr with a specific activity of 5.3 – 13.4 mCi/μg (195 – 497 MBq/μg).2 (link) Radiolabeling reactions were monitored using silica-gel impregnated glass-microfiber instant thin layer chromatography paper (iTLC-SG, iTLC-SA, Varian, Lake Forest, CA) and analyzed on a Bioscan AR-2000 radio-TLC plate reader using Winscan Radio-TLC software (Bioscan Inc., Washington, DC). All radiolabeling chemistry was performed with ultrapure water (>18.2 MΩ cm−1 at 25°C, Milli-Q, Millipore, Billerica, MA), and treatment of water or buffers with Chelex-100 resin was performed as indicated (1.2 g/L, 24 h) (BioRad Laboratories, Hercules, CA). Radioactivity in samples was measured using a Capintec CRC-15R dose calibrator (Capintec, Ramsey, NJ). For [89Zr]Zr4+ logD experiments, a Perkin-Elmer (Waltham, MA) Automated Wizard Gamma Counter was used for counting radioactivity.
Capintec crc 15r dose calibrator
Manufactured by Mirion Technologies
Sourced in Canada
The Capintec CRC-15R is a dose calibrator designed for measuring the radioactivity of radiopharmaceuticals and other radioactive samples. It provides accurate and reliable measurements of the activity levels of various isotopes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using capintec crc 15r dose calibrator
1
Production and Purification of High-Activity 89Zr
2
Radiolabeling of Nanoparticles with 89Zr
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the NP radiolabeling
and the preparation of the phospholipid chelator DSPE-DFO, similar
methods were used as previously described.19 (link),65 (link),66 (link)89Zr-oxalate was produced at
Memorial Sloan Kettering Cancer Center (New York) on an EBCO TR19/9
variable-beam energy cyclotron (Ebco Industries Inc., BC, Canada) via the 89Y(p,n)89Zr reaction and
purified in accordance with previously reported methods to yield 89Zr with a specific activity of 195–497 MBq/μg.66 (link) Activity measurements were made using a Capintec
CRC-15R Dose Calibrator (Capintec, Ramsey, NJ). 89Zr-oxalate
was neutralized with aqueous Na2CO3 (1 M), added
to a solution of 0.5 mol % DFO-bearing liposomes or nanoemulsions
in PBS, and stirred at 37 °C for 2 h. The labeled NPs were separated
from free 89Zr using a PD-10 desalting column (GE Healthcare),
with PBS as the eluent and collecting 0.5 mL fractions. The radiochemical
yield was typically >90%. The fractions containing most activity
(∼1.5–2.0
mL) were used for the experiments. These had a radiochemical purity
of >98%, as determined by SEC radio-HPLC analyses performed on
a Shimadzu
system equipped with a Superdex 10/300 SEC column and a A Lablogic
Scan-RAM radio-TLC/HPLC detector. A flow rate of 1 mL/min was used
with demiwater as the eluent.
and the preparation of the phospholipid chelator DSPE-DFO, similar
methods were used as previously described.19 (link),65 (link),66 (link)89Zr-oxalate was produced at
Memorial Sloan Kettering Cancer Center (New York) on an EBCO TR19/9
variable-beam energy cyclotron (Ebco Industries Inc., BC, Canada) via the 89Y(p,n)89Zr reaction and
purified in accordance with previously reported methods to yield 89Zr with a specific activity of 195–497 MBq/μg.66 (link) Activity measurements were made using a Capintec
CRC-15R Dose Calibrator (Capintec, Ramsey, NJ). 89Zr-oxalate
was neutralized with aqueous Na2CO3 (1 M), added
to a solution of 0.5 mol % DFO-bearing liposomes or nanoemulsions
in PBS, and stirred at 37 °C for 2 h. The labeled NPs were separated
from free 89Zr using a PD-10 desalting column (GE Healthcare),
with PBS as the eluent and collecting 0.5 mL fractions. The radiochemical
yield was typically >90%. The fractions containing most activity
(∼1.5–2.0
mL) were used for the experiments. These had a radiochemical purity
of >98%, as determined by SEC radio-HPLC analyses performed on
a Shimadzu
system equipped with a Superdex 10/300 SEC column and a A Lablogic
Scan-RAM radio-TLC/HPLC detector. A flow rate of 1 mL/min was used
with demiwater as the eluent.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!