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2 protocols using phenylethyl isothiocyanate

1

Comprehensive Analytical Protocol for Natural and Synthetic Compounds

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A mixture of C7–C30 normal alkanes in n-hexane (HPLC grade) was purchased from Beijing Huawei Ruike Chemical Co., Ltd. (Beijing, China); 6-undecanone (AR) was obtained from Tokyo Huacheng Industrial Pearl Club; sec-butylisothiocyanate-(96%), allyl isothiocyanate (95%), 3-(methylthio)propyl isothiocyanate(98%), benzyl isothiocyanate(97%), phenylethyl isothiocyanate(96%), allyl mercaptan(97%), diallyl sulfide(95%), diallyl disulphide(98%), carbon disulfide(97%), allyl chloride(96%), hexanoic acid(95%), 3-methylphenol(98%), and propylene glycol (99%) were purchased from Sigma-Aldrich Chemical Co. (Saint Louis, USA). Sudan Ⅲ and liquid paraffin were obtained from Beijing Chemical Reagent Co., Ltd. (Beijing, China).
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2

Isothiocyanate Compounds and Quercetin Analysis

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Ethyl isothiocyanate (97%), butyl isothiocyanate (99%), allyl isothiocyanate (99.7%), phenyl isothiocyanate (99%), 4-methoxyphenyl isothiocyanate (98%), 4-methylphenyl isothiocyanate (97%), benzyl isothiocyanate (98%), phenylEthyl isothiocyanate (98.5%), and quercetin (>95%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 4-Hydroxybenzyl isothiocyanate (98%) was purchased from Santacruz Biotechnologies (Santacruz, TX, USA). Thin-layer chromatography (TLC) plates (250 μm thickness, 2−25 μm particle size) were purchased from Macherey–Nagel (Macherey-Nagel, Düren, Germany). Sepabeads resin styrenic adsorbent (130 Å porosity) was obtained from Sorbtech (Peachtree Corners, GA, USA). LC-MS grade acetonitrile, water, and formic acid, and ACS-grade n-hexane, ethyl acetate, acetone, chloroform, and methanol were purchased from Sigma-Aldrich. Moringa seeds collected in West Bengal, India, were purchased from Amazon (Bellevue, WA, USA) in May 2019.
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