Haplotype tagging SNPs across C3 region were obtained from HapMap Project database for the Han Chinese population (http://hapmap.ncbi.nlm.nih.gov/ ). Eight SNPs were selected by the tagger-pairwise method with R square and MAF values greater than 0.80 and 0.10 respectively. This set of 8 SNPs captured 96% of alleles in the C3 locus with MAF larger than 0.1 and a mean r2 of 0.97. All SNPs were genotyped by TaqMan SNP Genotyping Assays (Applied Biosystems Inc., Foster City, CA) in the Light Cycler 480 Genotyping Master (Roche Diagnostics Inc., Mannhein, Germany) according to manufacturers’ protocols. All PCR amplifications were performed with the following thermal cycling conditions: 95 °C for 10 minutes followed by 40 cycles of 92 °C for 15 seconds, and 62 °C for 1.5 minutes. The HLA-B27 allele was detected by nested polymerase chain reaction (nPCR).
Light cycler 480 genotyping master
Manufactured by Roche
Sourced in Germany
The Roche Light Cycler 480 Genotyping Master is a versatile real-time PCR reagent kit designed for high-throughput genotyping applications. It provides reliable and sensitive detection of single nucleotide polymorphisms (SNPs) and other genetic variations.
Automatically generated - may contain errors
3 protocols using light cycler 480 genotyping master
1
Haplotype Tagging SNPs in C3 Region
2
EGFR Mutation Status Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melting curve analysis was performed as previously described (20 (link)). In brief, to analyze T790M mutation status, exon 20 of EGFR was first amplified by PCR from DNA by using the appropriate primers and the LightCycler 480 Genotyping Master (Roche Diagnostics, Mannheim, Germany), and then hybridized by using sensor and anchor probes. Human genomic DNA (Promega, Madison, WI, USA) was used as a wild-type EGFR control.
3
FKBP5 SNP rs1360780 Genotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples (7.5mL) were collected in ethylenediaminetetraacetic acid containing tubes at baseline and DNA was extracted using the Puregene whole blood DNA-extraction kit (Gentra Systems Inc.). The FKBP5 SNP rs1360780 was genotyped by using real-time PCR and subsequent melting curve analysis performed with a Lightcycler 480 Genotyping Master (Roche Applied Science, Mannheim, Germany). The minor allele frequency was 28%. Genotype distribution did not deviate from the Hardy-Weinberg equilibrium as indicated by a nonsignificant P value of the exact test (P=.110). The SNP could be successfully genotyped in all samples (call rate=100%).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!