Afatinib was acquired from Zhengzhou Acme Chem Co., Ltd. (Zhengzhou, China). Sitagliptin was procured from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). GLP-23-33 was obtained from Nanjing Peptide Biotech Ltd. (Nanjing, China). Gly-Pro-7-AMC was sourced from Sigma-Aldrich (St. Louis, MO, USA). The Invitrogen™ TRIzol reagent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). The Prime Script RT Reagent Kit and SYBR Premix Ex Taq Kit were purchased from Takara Biomedical Technology (Dalian, China). Total GLP-1 ELISA kit and total GLP-2 ELISA kit were acquired from CUSABIO Biotech CO., Ltd. (Wuhan, China), while the active GLP-1 (GLP-17-36) ELISA kit were from ALPCO (Salem, USA).
Sitagliptin
Manufactured by Yuanye Bio-Technology
Sourced in China
Sitagliptin is a pharmaceutical compound used as a laboratory reagent. It is a dipeptidyl peptidase-4 (DPP-4) inhibitor, which plays a role in glucose metabolism. The core function of Sitagliptin is to inhibit the DPP-4 enzyme, thereby affecting the regulation of blood glucose levels. Detailed information regarding its intended use or applications is not available.
Automatically generated - may contain errors
Lab products found in correlation
Sybr premix ex taq kit, by Takara Bio (1 mentions)
Invitrogen trizol reagent, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Biacore t200, by GE Healthcare (1 mentions)
Cm5 chip, by GE Healthcare (1 mentions)
Edc nhs, by GE Healthcare (1 mentions)
Pbs p, by GE Healthcare (1 mentions)
Ethanolamine, by GE Healthcare (1 mentions)
2 protocols using sitagliptin
1
Pharmacological Modulation of GLP-1 and GLP-2
2
Sitagliptin-Target Protein Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To verify the affinity and kinetics between sitagliptin and the novel target, we performed this experiment using Biacore T200 (GE Healthcare) instrument based on SPR, and used GraphPad Prism 8.0 software to visualize the data results (18 (link), 19 (link)).
Firstly, the amino acid sequence of the target protein was searched by Uniprot database, and the sequence was imported into Expasy (https://web.expasy.org/ ) website to calculate the isoelectric point of the protein (20 (link)). Then, the preconcentration experiment was carried out to determine the optimal coupling conditions. The target protein was coupled to CM5 chip (GE Healthcare) using the Immobilization module in Biacore T200 Control Software, and the reference channel was set to deduct the background. The chip was activated by EDC/NHS (GE Healthcare), and the uncoupling site was blocked by ethanolamine (GE Healthcare).
sitagliptin (HPLC ≥ 98%, Shanghai yuanye Bio-Technology Co., Ltd) was dissolved in DMSO (VWR) solution and diluted with PBS-P+ (GE Healthcare) solution to the desired compound concentration (1×PBS-P+, 5% DMSO). The affinity and kinetics of sitagliptin with the novel target protein were tested by LMW kinetics module in Biacore T200 Control Software, and extra wash after injection with 50% DMSO was added.
Firstly, the amino acid sequence of the target protein was searched by Uniprot database, and the sequence was imported into Expasy (
sitagliptin (HPLC ≥ 98%, Shanghai yuanye Bio-Technology Co., Ltd) was dissolved in DMSO (VWR) solution and diluted with PBS-P+ (GE Healthcare) solution to the desired compound concentration (1×PBS-P+, 5% DMSO). The affinity and kinetics of sitagliptin with the novel target protein were tested by LMW kinetics module in Biacore T200 Control Software, and extra wash after injection with 50% DMSO was added.
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