Rat leptin elisa kit

Before the sacrifice, blood was drawn from the tail vein, and the systolic blood pressure was measured afterwards. A total of 800 µL of blood were drawn into tubes containing L-heparin (Sarstedt, Nümbrecht, Germany). Following a 10 min, 3000 rpm centrifugation of the blood samples, the plasma was extracted and kept at −80 °C. Blood parameters (Supplementary Table S1) were evaluated as previously described [20 (link),21 (link),22 (link),23 (link),24 (link)]. Leptin concentration was evaluated by the colorimetric method using a specific kit (Rat Leptin ELISA Kit, Abcam ab100773, Cambridge CB2 0AX, UK).

The levels of plasma adipokines (leptin and adiponectin) were assessed using Rat Leptin Elisa kit (Abcam®–ab100773, Cambridge, MA) and Rat Adiponectin Elisa kit (Abcam®–ab108784, Cambridge, MA) respectively. Measurements were conducted according to manufacturer's instructions. Briefly, standards, controls, and samples were placed into the wells and incubated 2 h at room temperature. After washing 5 times, the enzyme-linked polyclonal antibody specific for rats adipokines of interest was added to the wells and then, after washing, the substrate solution was added. The enzyme reaction was read at 450 nm (correction wavelength set at 570 nm). The samples values were read off the standard value. Values below the standard curve were excluded from the final analysis. Plasma triglyceride levels were determined by MultiCarein™ triglyceride test strips and by a specific analyzer (Biochemical Systems International, Arezzo, Italy).