The animals used in this study were male C57BL/6 mice that were either PND 28 (adolescents) or PND 56 (adults) at the onset of drinking, to maintain consistency with our previous experiments. C57BL/6 mice are commonly used in alcohol studies due to their propensity to consume alcohol (Hwa et al., 2011 (link); Le et al., 1994 (link)). Animals were housed in a climate-controlled vivarium under a reverse light/dark cycle (lights off at 10am) in groups of 4 per cage. Animals were identified using small animal ear tags (Stoelting, Wood Dale, IL). Food and water were available ad libitum, except during the 2-h alcohol-drinking period. The study consisted of 2 age groups (adults and adolescents), 2 drinking groups (alcohol or water), and 3 treatment groups (MTEP, CDPPB, or vehicle); n = 11/group.
Small animal ear tags
Manufactured by Stoelting
Sourced in United States
Small animal ear tags are identification devices that are attached to the ears of small animals, such as rodents, for the purpose of tracking and identification. They serve as a reliable method to mark and distinguish individual animals within a research or laboratory setting.
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Lab products found in correlation
4 protocols using small animal ear tags
1
Alcohol Drinking Behavior in Adolescent and Adult Mice
2
Voluntary Alcohol Consumption in Mice
3
Investigating Alcohol Consumption in Mice
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This study used 60 adult male C57BL/6J mice (Jackson Laboratories, Sacramento, CA) that
were eight weeks of age at the onset of drinking. Animals were housed in groups of four in
standard Plexiglas cages, in a temperature-controlled vivarium (23℃), under a 12-h reverse
light/dark cycle (lights off at 10 a.m.). Animals were identified using small animal ear
tags (Stoelting, Wood Dale, IL). Food and water were available ad
libitum, with the exception of the 2-h alcohol-drinking period. The study had a 2
(alcohol or water) × 3 (vehicle, buspirone, or MTEP) factorial design, with
n = 10/group. All experiments were conducted in compliance with the
National Institutes of Health Guide for Care and Use of Laboratory Animals (NIH
Publication No. 80–23, revised 2014) and approved by the IACUC of the University of
California, Santa Barbara.
were eight weeks of age at the onset of drinking. Animals were housed in groups of four in
standard Plexiglas cages, in a temperature-controlled vivarium (23℃), under a 12-h reverse
light/dark cycle (lights off at 10 a.m.). Animals were identified using small animal ear
tags (Stoelting, Wood Dale, IL). Food and water were available ad
libitum, with the exception of the 2-h alcohol-drinking period. The study had a 2
(alcohol or water) × 3 (vehicle, buspirone, or MTEP) factorial design, with
n = 10/group. All experiments were conducted in compliance with the
National Institutes of Health Guide for Care and Use of Laboratory Animals (NIH
Publication No. 80–23, revised 2014) and approved by the IACUC of the University of
California, Santa Barbara.
4
Binge Drinking in Adolescent Mice
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The subjects in this study were male C57BL/6J mice, purchased from The Jackson Laboratory (Sacramento, CA, United States). Mice were allowed 7 days to acclimate to our vivarium and were either PND 28 (adolescents) or PND 56 (adults) at the onset of our binge-drinking procedures. Animals were identified using small animal ear tags (Stoelting, Wood Dale, IL, United States) and housed in age-matched groups of 4 in a climate-controlled vivarium under a reverse light/dark cycle (lights off at 10:00 h). Food and water were available ad libitum, except during the 2-h alcohol-drinking period. The study design consisted of 2 age of drinking-onset groups (adults versus adolescents), 2 drinking groups (alcohol versus water), and 3 intra-AcbSh treatment groups (0, 1 or 10 μg MTEP). The sample size was 12 per group.
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