Alexa fluor 546 goat anti mouse igg

Cycloheximide (CHX), polybrene, polyethylenimine (PEI), puromycin, and Oil Red O were from Sigma–Aldrich (St. Louis, MO, U.S.A.), MG132 was from BD Biosciences (Franklin Lakes, NJ, U.S.A.), and Polyjet, Lipo2000 and TRIzol reagent were from Invitrogen (Carlsbad, CA, U.S.A.). RIPA buffer and PCR 2X mix were from Yeasen (Shanghai, China). Rapamycin was from MCE (NJ, USA). AICAR was from CSNpharm (Chicago, MI, USA). Sodium caprylate was from Sangon (Shanghai, China). Antibodies against Flag tag and α-tubulin were from Sigma–Aldrich; antibodies against Myc tag and mouse IgG were from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.); antibodies against GFP, HUWE1, HSP90, CREB, phosphorylated CREB, and TP53 were from Cell Signaling Technology (Boston, MA, USA); antibodies against HA and GAPDH were from ABclonal (Boston, MA, U.S.A.); antibodies against PAQR9, PPARα, Alexa Fluor 546 goat anti-mouse IgG, and Alexa Fluor 546 goat anti-rabbit IgG were from Abcam (Cambridge, U.K.); antibody against ubiquitin was from Santa Cruz Biotechnology (TX,U.S.A.).

The materials and antibodies used in this study were purchased as follows: anti-HSL from Cell Signaling Technology (4107s, Danvers, MA, USA); anti-phospho-HSL (Ser660) from Cell Signaling Technology (4126s); anti-phospho-PKA substrate from Cell Signaling Technology (9621s); Anti-GAPDH from ABclonal (AC033, Boston, MA, USA); anti-Flag tag from Sigma–Aldrich (F3165, St. Louis, MO, USA); anti-GM130 from Abcam (ab52649, Cambridge, UK); anti-PDE4D from Abcam (ab14613); anti-perilipin1 from Vala Sciences (4854, San Diego, CA, USA); anti-p-perilipin1 from Vala Sciences (4856); anti-α-tubulin from Sigma–Aldrich (T6199); anti-Myc tag from Santa Cruz Biotechnology (sc-40, Santa Cruz, CA); anti-GFP from Santa Cruz Biotechnology (sc-9996); cycloheximide (CHX) and polyethylenimine (PEI) from Sigma–Aldrich (C7698); Alexa Fluor 546 goat anti-mouse IgG from Abcam (A11003); Alexa Fluor 546 goat anti-rabbit IgG from Abcam (A11035); Alexa Fluor 488 goat anti-mouse IgG from Abcam (A11029); Alexa Fluor 488 goat anti-rabbit IgG from Abcam (A11034). The selective PDE4D inhibitor D-159687 (CSN23886) was from CSNpharm (Chicago, MI, USA).