Transfected CRC cells were resuspended using phosphate buffer saline (PBS). The CRC cells were stained with Annexin V combined fluorescein isothiocyanate (FITC) and propidine iodide (PI; Solarbio, Beijing, China) in dark. After washing with PBS, the apoptotic cells (FITC+/PI±) and non-apoptotic cells were subjected to the analysis by the flow cytometer (BD Biosciences, San Jose, CA, USA).
Propidine iodide
Manufactured by Solarbio
Sourced in China
Propidine iodide is a chemical compound used in various laboratory applications. It is a staining agent commonly employed in microscopy and cell biology research to visualize and differentiate cellular structures.
Automatically generated - may contain errors
2 protocols using propidine iodide
1
Apoptosis Assay of CRC Cells
2
Annexin V-FITC Apoptosis Assay
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Annexin V-FITC Apoptosis Detection Kit (Invitrogen) was used to determine the apoptosis of CC cells. CC cells were harvested and re-suspended in 500µL Annexin V-fluorescein isothiocyanate (FITC) binding buffer. 5 µL Annexin V-FITC and 5 µL propidine iodide (PI; Solarbio, Beijing, China) were mixed with the CC cells in the dark for 10 min. The apoptotic cells (FITC+, PI±) were recognized through using flow cytometry system (BD Biosciences, San Jose, CA, USA).
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