Tsq vantage triple quadrupole qqq mass spectrometer

Manufactured by Thermo Fisher Scientific

Sourced in Denmark

The TSQ Vantage triple quadrupole (QQQ) mass spectrometer is a high-performance analytical instrument designed for sensitive and selective detection and quantification of target analytes in complex matrices. It utilizes triple quadrupole technology to provide accurate and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Nanoacquity uplc system, by Waters Corporation (2 mentions) Easy column, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TSQ Vantage (98 citations) TSQ Vantage mass spectrometer (25 citations) TSQ Vantage triple (5 citations) Triple quadrupole (QqQ) mass spectrometer (4 citations) TSQ Vantage triple quadrupole (3 citations) TSQ Vantage QqQ mass spectrometer (2 citations)

4 protocols using tsq vantage triple quadrupole qqq mass spectrometer

Plasma Glycopeptide Profiling via SRM-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

All SRM analyses were performed on
a TSQ Vantage triple quadrupole (QQQ) mass spectrometer (Thermo Scientific)
at the University of Washington Proteomics Resource. The mass spectrometer
was coupled to a nanoelectrospray ionization source and a Waters nanoACQUITY
UPLC system. Glycopeptides from 1 μL of original plasma (∼1
μg peptides) were loaded onto a C18 trap column (20 mm long,
75 μm ID) and separated by a 150 mm C18 column (75 μm
ID) over a 60 min 2–35% linear acetonitrile gradient. Spray
voltage was set at 1700 V. Scheduled SRM was performed with 5 min
retention time windows for most of the peptides and an instrument
cycle time of 2000 ± 500 ms. Dwell times were varied depending
on the number of concurrent transitions; in all cases they were at
least 10 ms.

Pan C., Zhou Y., Dator R., Ginghina C., Zhao Y., Movius J., Peskind E., Zabetian C.P., Quinn J., Galasko D., Stewart T., Shi M, & Zhang J. (2014). Targeted Discovery and Validation of Plasma Biomarkers of Parkinson’s Disease. Journal of Proteome Research, 13(11), 4535-4545.

+ Open protocol

+ Expand

Liquid Chromatography-Mass Spectrometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The liquid chromatography (LC)-MS system consisted of a Proxeon EASY nano-LC (Proxeon, Odense, Denmark) coupled to a TSQ-Vantage triple quadrupole (QQQ) mass spectrometer (Thermo Fisher Scientific, Waltham, MA). The LC was C18-based reverse phase separation with a 2-cm trap column and a 10-cm analytical column (EASY column, Thermo). Columns were equilibrated with 100% buffer A and peptides were eluted with a 40 min linear gradient of buffer A and buffer B (AcN, 5% H₂O, 0.1% HCOOH), until 40% B was reached. MS raw data files were imported into Skyline and each file was quality checked by visual inspection of retention time and ranking of transitions for each peptide. A more detail description can be found in the Data S1.

Fernández-Guerra P., Birkler R.I., Merinero B., Ugarte M., Gregersen N., Rodríguez-Pombo P., Bross P, & Palmfeldt J. (2014). Selected reaction monitoring as an effective method for reliable quantification of disease-associated proteins in maple syrup urine disease. Molecular Genetics & Genomic Medicine, 2(5), 383-392.

+ Open protocol

+ Expand

Quantitative Plasma Glycopeptide Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All SRM analyses were performed on a TSQ Vantage triple quadrupole (QQQ) mass spectrometer (Thermo Scientific) at the University of Washington Proteomics Resource. The mass spectrometer was coupled to a nano-electrospray ionization source and a Waters nanoACQUITY UPLC system. Glycopeptides from 1 μl original plasma (~ 1 μg peptides) were loaded onto a C18 trap column (20 mm long, 75μm ID) and separated by a 150 mm C18 column (75μm ID) over a 60-min 2-35% linear acetonitrile gradient. Spray voltage was set at 1700 V. Scheduled SRM were performed with 5-min retention time windows for most of the peptides and an instrument cycle time of 2000 ± 500ms. Dwell times were varied depending on the number of concurrent transitions; in all cases they were at least 10 ms.

+ Open protocol

+ Expand

Pentazoles Fragmentation Analysis by MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents and solvents were purchased from Aladdin and Energy Chemical as analytical grade and used without further purification. All experiments used deionized water with a resistivity of 18.2 MΩ.
For the cleavage experiments, these pentazoles reaction liquid were dissolved in CH₃OH, and infused into the spectrometer’s ion source at 10 μL·min⁻¹ with a syringe pump. In tandem mass spectrometry (MS/MS) experiments, the desired negative-ion or positive-ion peaks are mass-selected and subjected to product ion mass analysis following collision-induced dissociation (CID) at variable collision voltages using N₂ as the collision gas.
Mass spectrometric ionization was carried out in electrospray ionization source (ESI) ionization mode with a source temperature of 250 °C. The detection was carried out on a TSQ Vantage triple quadrupole (QqQ) mass spectrometer (Thermo Fisher Scientific), equipped with an ESI turbo spray ionization source. The entire system was controlled via Xcalibur 2.2 software and data were processed using TraceFinder 3.1.

Hou T., Yuan X., Jiang S., Xu Z., Zhang X., Lu M, & Xu Y. (2024). Experimental detection of the diamino-pentazolium cation and theoretical exploration of derived high energy materials. Scientific Reports, 14, 10120.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!