Atorvastatin

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Atorvastatin is a selective and competitive inhibitor of HMG-CoA reductase, the enzyme that catalyzes the rate-limiting step in cholesterol biosynthesis. It is used as a laboratory tool to study cholesterol metabolism and related biological processes.

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Lab products found in correlation

Fluvastatin, by Selleck Chemicals (2 mentions) Pravastatin, by Selleck Chemicals (2 mentions) Simvastatin, by Selleck Chemicals (2 mentions) 293t cell, by American Type Culture Collection (1 mentions) Simvastatin, by Santa Cruz Biotechnology (1 mentions) Cholesterol, by Santa Cruz Biotechnology (1 mentions) Methyl β cyclodextrin mcd, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Cholesterol, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Lovastatin, by Santa Cruz Biotechnology (1 mentions) Abiraterone acetate, by Selleck Chemicals (1 mentions) Pravastatin, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Anti rhoa, by Merck Group (1 mentions) Goat anti rabbit igg, by Merck Group (1 mentions)

5 protocols using atorvastatin

Hormone-sensitive Prostate Cancer Cell Lines

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The hormone sensitive PC cell lines, LNCaP and 22RV1 were used for the in vitro studies. LNCaP and 22RV1 cells were maintained in RPMI 1640 and supplemented with 10% FBS and antibiotics. For the cell proliferation studies, all PC cells were cultured in Phenol-Red free RPMI 1640/10% charcoal-stripped FBS. 293T cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco's Modified Eagle's Medium (DMEM) with 10% FBS and antibiotics. All cell lines were regularly screened for mycoplasma (Sigma Venor GeM Mycoplasma Detection Kit). DHEAS was obtained from BioVender R&D Products. Atorvastatin was obtained from Santa Cruz Biotechnology, and fluvastatin, pravastatin and simvastatin were purchased from Selleckchem.

Harshman L.C., Wang X., Nakabayashi M., Xie W., Valenca L., Werner L., Yu Y., Kantoff A.M., Sweeney C.J., Mucci L.A., Pomerantz M., Lee G.S, & Kantoff P.W. (2015). Statin use at the time of initiation of androgen deprivation therapy and time to progression in patients with hormone-sensitive prostate cancer. JAMA oncology, 1(4), 495-504.

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Preparation of Statin and Cholesterol Treatments

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Simvastatin and atorvastatin (both from Santa Cruz Biotechnology, Inc.) were dissolved in DMSO (Sigma-Aldrich; Merck KGaA) and dilutions were made for appropriate treatment conditions at 1% final concentration of DMSO. Cholesterol (Santa Cruz Biotechnology, Inc.) was dissolved in chloroform and a desired concentration of Cholesterol was left after being evaporated using nitrogen gas. The Cholesterol was then resuspended in methyl-β-cyclodextrin (MCD; Sigma-Aldrich; Merck KGaA) at a ratio of 1:4.

Seeree P., Janvilisri T., Kangsamaksin T., Tohtong R, & Kumkate S. (2019). Downregulation of ABCA1 and ABCG1 transporters by simvastatin in cholangiocarcinoma cells. Oncology Letters, 18(5), 5173-5184.

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Zebrafish Embryo Cardiac and Lens Assays

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One dpf embryo was manually dechorionated, and 10 embryos were placed in one well of a 24-well plate (polystyrene, tissue culture grade) with 1 mL of 0.3× Danieau water. Then, 50 μM dexamethasone (Sigma, D1756) diluted in 0.3× Danieau water was treated from 1 to 4 dpf to examine the cardiac phenotypes. For stain experiments, 2.5 or 5 μM atorvastatin (Santa Cruz Biotechnology, sc-337542A) was treated from 1 to 4 dpf to observe lens defects, and 4 μM lovastatin (Santa Cruz Biotechnology, sc-200850A) was treated for 16 h before examining lens abnormalities at 4 dpf.

Park J., MacGavin S., Niederbrach L, & Mchaourab H.S. (2023). Interplay between Nrf2 and αB-crystallin in the lens and heart of zebrafish under proteostatic stress. Frontiers in Molecular Biosciences, 10, 1185704.

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Prostate Cancer Cell Lines Comparison

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The hormone sensitive prostate cancer cell lines, LNCaP and 22RV1, were employed in the study. LNCaP and 22RV1 cells were maintained in RPMI 1640 and supplemented with 10% FBS and antibiotics. The stable inducible shSLCO2B1-expressing cell lines were used as previously described and maintained in medium containing puromycin^{1 (link)}. All cell lines were regularly screened for mycoplasma (Sigma Venor GeM Mycoplasma Detection Kit). Atorvastatin was purchased from Santa Cruz Biotechnology. Abiraterone acetate, fluvastatin, pravastatin and simvastatin were purchased from Selleckchem.

Harshman L.C., Werner L., Tripathi A., Wang X., Maughan B.L., Antonarakis E.S., Nakabayashi M., McKay R., Pomerantz M., Mucci L.A., Taplin M.E., Sweeney C.J., Lee G.S, & Kantoff P.W. (2017). The impact of statin use on the efficacy of abiraterone acetate in patients with castration-resistant prostate cancer. The Prostate, 77(13), 1303-1311.

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Neonatal Rat Metabolism and Insulin Signaling

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Neonatal Sprague-Dawley (SD) rats (age 24 h, male and female) were provided by the Experimental Animal Center of China Medical University. Atorvastatin, pravastatin, and rosuvastatin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The insulin purchased from the Sigma-Aldrich (St Louis, MO, USA). Rabbit anti-GLUT4, anti-pIRS-1, anti-RhoA, anti-β-actin, and goat anti-rabbit IgG antibodies were purchased from Sigma-Aldrich.

Jiang Z., Yu B, & Li Y. (2016). Effect of Three Statins on Glucose Uptake of Cardiomyocytes and its Mechanism. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 2825-2830.

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