Bx53 microscope

Manufactured by Olympus

Sourced in Japan, United States, Germany, China, United Kingdom, Australia, Netherlands, Denmark, Panama

The BX53 microscope is a high-performance optical microscope designed for a variety of laboratory applications. It features a sturdy, ergonomic design and advanced optical components to provide clear, detailed images. The BX53 is capable of brightfield, darkfield, and polarized light microscopy techniques.

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Lab products found in correlation

Dp72 digital camera, by Olympus (25 mentions) Cellsens imaging software, by Olympus (19 mentions) Dp73 camera, by Olympus (19 mentions) Cellsens software, by Olympus (13 mentions) Dp72 camera, by Olympus (12 mentions) Image pro plus 6, by Media Cybernetics (12 mentions) Schiff s reagent, by Fujifilm (11 mentions) Cellsens dimension 1, by Olympus (11 mentions) Hoechst 33342, by Thermo Fisher Scientific (11 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (10 mentions) Mayer s hematoxylin solution, by Fujifilm (9 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (9 mentions) Dp26 camera, by Olympus (8 mentions) Dp74 camera, by Olympus (7 mentions) Dp21 digital camera, by Olympus (7 mentions) Ceros 2 sperm analysis system, by Hamilton Thorne (6 mentions) Dp80 digital camera, by Olympus (6 mentions) Triton x 100, by Merck Group (6 mentions) Cellsens standard software, by Olympus (6 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (6 mentions)

1 318 protocols using bx53 microscope

Microplastic Presence in Ecuadorian Marine Organisms

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To analyse plastic presence in marine organisms of human consumption, 15 specimens of each of the 16 different species collected, including molluscs, fish and crustaceans were bought across the most representative market ports in all four provinces (El Oro, Santa Elena, Manabí and Esmeraldas) evaluated in the Pacific coast of Ecuador (see Supplementary S1), under the same permit mentioned above. They were preserved frozen at – 20 °C. Samples were then dissected and tissue from the digestive tract and the dorsal muscle were investigated for each specimen. The collected samples were analysed in a BX53 Olympus microscope coupled with a microscale to visually quantify the presence of microplastic particles over 200 μm.
For muscle inspection, 0.5 cm³-muscle tissue fragments were imbibed in paraffin. These preparations were tanned with hematoxylin and eosin (H–E) technique and cut with a microtome³². Tissue slices were then prepared on microscope plates using Entellan resin and inspected for microplastic presence under the BX53 Olympus microscope. The figure presenting the concentrations of microplastic particles in marine organisms was made on Adobe Acrobat DC Pro (https://acrobat.adobe.com); organism illustrations were obtained at www.pexels.com (free access and use) and manually adjusted to the figure.

Alfaro-Núñez A., Astorga D., Cáceres-Farías L., Bastidas L., Soto Villegas C., Macay K, & Christensen J.H. (2021). Microplastic pollution in seawater and marine organisms across the Tropical Eastern Pacific and Galápagos. Scientific Reports, 11, 6424.

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Quantifying Lung Metastases in Breast Cancer

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Lungs excised from mice injected with either MIV-shSCR or MIV-INHBA breast cancer cells were fixed in 4% paraformaldehyde (PFA) and embedded in paraffin. Transverse tissue sections (8 μm-thick) were produced using an SRM200 microtome (Sakura), followed by staining with hematoxylin and eosin (H&E) using standard methodology. Bright-field images of stained slides were obtained using an Olympus BX53 microscope. For immunofluorescence analysis, tissue sections were re-hydrated and incubated in blocking solution (10% donkey serum, 3% FBS in PBS). Then sections were stained overnight with primary mouse anti-INHBA (1:50) and mouse anti-IL13Rα2 (1:50) antibodies (Santa Cruz Biotechnology) followed by incubation with secondary Alexa-fluor 488 donkey anti-mouse antibodies (1:300) and DAPI. Images were captured using an Olympus BX53 microscope. Average size of lung metastases was calculated from histology images using Image J software.

Kalli M., Mpekris F., Wong C.K., Panagi M., Ozturk S., Thiagalingam S., Stylianopoulos T, & Papageorgis P. (2019). Activin A Signaling Regulates IL13Rα2 Expression to Promote Breast Cancer Metastasis. Frontiers in Oncology, 9, 32.

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Murine Lung Histological Analysis

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Mice were thoracotomized under profound anesthesia, the pulmonary circulation flushed with saline and then the lungs were excised. One of the lungs was perfused with 10% neutral buffered formalin, fixed in formalin for 24 h and then embedded in paraffin. Tissue specimens of 5 µm were prepared, stained with hematoxylin-eosin and then examined under a light microscopy (Olympus BX53 microscope; Tokyo, Japan). An investigator blinded to the treatment group counted the number of inflammatory cells in a blinded fashion; at least five microscopic fields were counted per mouse. Cell counting in the histological sections was performed using the Olympus BX53 microscope with a plan objective, combined with an Olympus DP70 digital camera (Tokyo, Japan) and the WinROOF image processing software (Mitani Corp., Fukui, Japan) for Windows.

Baffour Tonto P., Yasuma T., Kobayashi T., D’Alessandro-Gabazza C.N., Toda M., Saiki H., Fujimoto H., Asayama K., Fujiwara K., Nishihama K., Okano T., Takeshita A, & Gabazza E.C. (2019). Protein S is Protective in Acute Lung Injury by Inhibiting Cell Apoptosis. International Journal of Molecular Sciences, 20(5), 1082.

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Morphological Examination of Adult Insect Specimens

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All adult specimens were obtained after by rearing from immature stages. Adult external morphology was examined by using a Leica M-205C stereomicroscope, and photographs were taken with a Leica DFC-450 digital camera connected to a Leica M-205C stereomicroscope. Genitalia were prepared following the methods of Li and Zheng (1996) . Dissections of genitalia were conducted under an Olympus SZX-7 stereomicroscope. Genital morphology was examined with an Olympus BX-53 microscope, and the illustrations were prepared by using an Olympus DP-26 digital camera connected to the Olympus BX-53 microscope. Terminology follows Kumata (1998) and Kumata et al. (1988) .
All specimens studied are deposited in the

Insect Collection, Department of Bioscience and Biotechnology, Changzhi College, Changzhi, Shanxi, China

(ICCC).

Bai H., Xu J, & Dai X. (2016). Two new and one newly recorded species of Gracillariidae from China (Lepidoptera). ZooKeys.

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Evaluation of Pollen Viability and Anther Morphology in BS366 under Cold Stress

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Photographs of the flower tissue for BS366 under cold and control environments were obtained using ZEISS SteREO Discovery, V20. To evaluate pollen viability, cold and control anthers were separately crushed, stained with 1% iodine-potassium iodide (I₂-KI) solution and photographed under an Olympus BX-53 microscope (Tokyo, Japan). For microspore and anther phenotype observation, anthers and spikelets from the corresponding developmental stages of BS366, from meiosis to the mature pollen stage under both control and cold conditions, were collected and fixed in FAA solution (formaldehyde: glacial acetic acid: 50% ethanol =5: 5: 9). The anthers were separated from the young spikes, mashed with tweezers to release the pollen, and dyed with improved carbol fuchsin solution. Photographs of microspores and pollen were obtained using an Olympus BX-53 microscope (Tokyo, Japan). For the anther phenotype analysis, the anthers were fixed in FAA solution, removed from the FAA fixative, dehydrated in an ethanol series, and then embedded in paraffin. Tissue sections were cut transversely from the wax-embedded anthers and stained using safranin O-fast green. The anther morphology was analyzed with a scanning electron microscope (HITACHI SU8100).

Liu Y.J., Li D., Gong J., Wang Y.B., Chen Z.B., Pang B.S., Chen X.C., Gao J.G., Yang W.B., Zhang F.T., Tang Y.M., Zhao C.P, & Gao S.Q. (2021). Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366. BMC Genomics, 22, 911.

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Pollen Grain and Anther Microstructure Analysis

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ospdt1 plants and flowers were examined under an Olympus MVX10 stereomicroscope and photographed using a Canon EOS 5D digital camera. After staining with 1% (w/v) I₂-KI, mature pollen grains were examined under an Olympus BX53 microscope. Anthers at different developmental stages were fixed in 4% (w/v) paraformaldehyde and 0.25% (v/v) glutaraldehyde in 0.1-M sodium phosphate buffer (pH 7.2) and postfixed in 1% (v/v) osmic acid. Following dehydration in a graded ethanol series and acetone replacement, anthers were embedded in Epon 812 resin. Transverse sections (2–4 µm thickness) were cut with a Leica RM2265 microtome, stained with 1% (w/v) toluidine blue O, and examined under an Olympus BX53 microscope. For TEM, ultra-thin sections cut using a Leica UC6 microtome were double-stained with uranyl acetate and lead citrate aqueous solution and examined under a Hitachi H-7500 transmission electron microscope. For SEM, mature pollen grains were examined under a Hitachi SU3500 scanning electron microscope.

Yin W., Yang H., Wang Y., Feng P., Deng Y., Zhang L., He G, & Wang N. (2022). Oryza sativa PECTIN DEFECTIVE TAPETUM1 affects anther development through a pectin-mediated signaling pathway in rice. Plant Physiology, 189(3), 1570-1586.

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Histological Analysis of Plant Tissues

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For histological analysis, samples of hand‐sectioned, fresh stems were stained with either Toluidine blue O, phloroglucinol‐HCL, or Mäule stain to visualise the cell walls, according to the methods outlined by Mitra & Loque (2014 ), with an Olympus BX53 microscope (Shinjuku, Tokyo, Japan) under bright field. At least three plant stems from three independent transformed lines were sectioned and measured for tissue organisation analyses.
For analysis of primary roots and pistils, phloroglucinol‐HCL staining was used. After staining, images were acquired with an Olympus BX53 microscope under bright field. At least three plants from three independent transgenic lines were sectioned and stained, with one representative image shown (Fig. S3).
A Zeiss LSM 780 laser scanning confocal microscope (Oberkochen, Germany) was used to detect lignin in fresh stem sections at growth stage 6.0 under ultraviolet (UV) light (excitation 405 nm, emission 440 nm, detection 404–476 nm). Yellow fluorescent protein (YFP) signals were detected in fresh stem sections using the Zeiss LSM 780 laser scanning confocal microscope (excitation 514 nm, emission 550 nm, detection 525–574 nm; LIMS Bio‐imaging platform, La Trobe University). At least three plants from three independent transgenic lines were imaged, with one representative image shown (Figs 1, 4).

Ma Y., MacMillan C.P., de Vries L., Mansfield S.D., Hao P., Ratcliffe J., Bacic A, & Johnson K.L. (2022). FLA11 and FLA12 glycoproteins fine‐tune stem secondary wall properties in response to mechanical stresses. The New Phytologist, 233(4), 1750-1767.

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Quantification of Dopaminergic Neurons and Fibers

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DA neurons labeled with TH in the substantia nigra (SN) were identified and counted at 20 × magnification at three SN sections per mouse located approximately at Bregma − 3.16 mm, − 3.40 mm, and − 3.64 mm [33 , 37 (link)] using the Olympus BX53 microscope and CellSens software. The data were presented as a percentage of TH⁺ cells remaining in the ipsilateral side compared to the mean of the TH⁺ cells in the contralateral intact hemisphere of sham/CTRL diet mice corresponding to 100%.
The density of TH⁺ and DAT⁺ fibers was evaluated in 3 striatal sections per mouse located between AP + 0.62 to + 1.18 relative to bregma [33 ]. High-resolution images were obtained at 4 × magnification (Olympus BX53 microscope). The optical density of the striatal fibers was analyzed by ImageJ (NIH, USA) and normalized to the corpus callosum for each picture. The data is presented as percentage of fiber density in the ipsilateral side compared to the mean of the fiber density in the contralateral intact hemisphere of sham/CTRL diet mice corresponding to 100%.

Elabi O.F., Cunha J.P., Gaceb A., Fex M, & Paul G. (2021). High-fat diet-induced diabetes leads to vascular alterations, pericyte reduction, and perivascular depletion of microglia in a 6-OHDA toxin model of Parkinson disease. Journal of Neuroinflammation, 18, 175.

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Evaluating Pollen Viability in BS366 Plants

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Ten BS366 plants were used for the pollen viability evaluation. Anthers from three middle florets of one spike were mixed into three replicates to assess the pollen viability per plant. Anthers were separately crushed and stained with 1% iodine-potassium iodide (I₂-KI) solution. The fertile pollen ratio of each replicate was calculated for the cold- and control-treated BS366. BS366 plants of uniform growth were selected and then bagged at heading stage. Ten individual plants with three main spikes were chosen for phenotype evaluation. Spikelet seed-setting rate was calculated using (Number of spikelet seeds per ear/Total number of spikelets per ear) × 100% at maturity stage. For microspore phenotype observation, anthers at respective stages under both conditions were collected and fixed in FAA solution (formaldehyde:glacial acetic acid:50% ethanol = 5:5:9). To evaluate pollen viability, cold and control anthers were separately crushed, stained with 1% I₂-KI solution and photographed under an Olympus BX-53 microscope (Tokyo, Japan). For microspore phenotype observation, the anthers were mashed with tweezers to release the pollen and dyed with improved carbol fuchsin solution. Photographs of microspores and pollen were obtained using an Olympus BX-53 microscope (Tokyo, Japan).

Liu Y., Li D., Zhang S., Zhang L., Gong J., Li Y., Chen J., Zhang F., Liao X., Chen Z., Wang Y., Pang B., Ma J., Chen X., Gao J., Zhao C, & Gao S. (2022). Integrated Analysis of Microarray, Small RNA, and Degradome Datasets Uncovers the Role of MicroRNAs in Temperature-Sensitive Genic Male Sterility in Wheat. International Journal of Molecular Sciences, 23(15), 8057.

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Evaluating Pollen Characteristics in Male Flowers

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To record the flowering time of male flowers, the first flower and floral growth were observed until anthesis. The observations were conducted in autumn 2016, spring 2017, and autumn 2017.
To test pollen tube elongation, pollen was obtained from 0 DPA male flowers and spread onto medium consisting of 10% (w/v) sucrose, 0.5% (w/v) boric acid, and 0.5% (w/v) Phytagel (Sigma) at room temperature. At least 20 dimensions of the images were obtained with an Olympus BX53 microscope taken after 45–60 min. To test pollen vigor, pollen was obtained from 0 DPA male flowers. The pollen was spread onto medium consisting of 1% (w/v) triphenyltetrazolium chloride solution (0.2 g of triphenyltetrazolium chloride and 12 g of sucrose dissolved in 20 ml of distilled water). The sample was kept at room temperature for 2 h and then examined with an Olympus BX53 microscope.

Cai Y., Bartholomew E.S., Dong M., Zhai X., Yin S., Zhang Y., Feng Z., Wu L., Liu W., Shan N., Zhang X., Ren H, & Liu X. (2020). The HD-ZIP IV transcription factor GL2-LIKE regulates male flowering time and fertility in cucumber. Journal of Experimental Botany, 71(18), 5425-5437.

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