Pericytes were transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit (Lonza, Switzerland, EU, Cat# VPI-1001) as described [16 (link)]. For ocln overexpression, cells were transfected with 1 μg PCMV3-OCLN plasmid (Sino Biological, Wayne, PA, Cat# HG15134-UT) or with PCMV3 (Sino Biological, Cat# CV011) as a negative control. For gene silencing, cells were transfected with 0.5 μg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer OAS1-siRNAs (OriGene, Rockville, MD, USA, Cat# SR303266), 3 unique 27mer OAS2-siRNAs (OriGene, Cat# SR303267), 3 unique 27mer OAS3-siRNAs (OriGene, Cat# SR303268), 3 unique 27mer OASL-siRNAs (OriGene, Cat# SR305676), 3 unique 27mer RNaseL-siRNAs (OriGene, Cat# SR304081), 3 unique 27mer occludin-siRNAs (OriGene, Cat# SR303274), 3 unique 27mer TJP1-siRNAs (OriGene, Cat# SR322042), or trilencer-27 universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) as a negative control.
Pcmv3 ocln plasmid
Manufactured by Sino Biological
The PCMV3-OCLN plasmid is a laboratory research tool designed for the expression of the occludin (OCLN) protein in various cell lines. The plasmid contains the OCLN gene under the control of a cytomegalovirus (CMV) promoter, allowing for the efficient expression of the target protein. This plasmid is intended for use in research applications that require the production and study of the occludin protein.
Automatically generated - may contain errors
Lab products found in correlation
Pcmv3, by Sino Biological (2 mentions)
Amaxa nucleofector technology, by Lonza (2 mentions)
Basic nucleofector kit, by Lonza (2 mentions)
27mer oas1 sirnas, by OriGene (2 mentions)
27mer oas2 sirnas, by OriGene (2 mentions)
27mer oas3 sirnas, by OriGene (2 mentions)
27mer oasl sirnas, by OriGene (2 mentions)
27mer rnasel sirnas, by OriGene (2 mentions)
27mer occludin sirnas, by OriGene (2 mentions)
27mer tjp1 sirnas, by OriGene (2 mentions)
2 protocols using pcmv3 ocln plasmid
1
Pericyte Transfection and Gene Silencing
2
Transfection and Gene Silencing in Pericytes
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Pericytes were transfected using Amaxa Nucleofector Technology and the Basic Nucleofector Kit (Lonza, Switzerland, EU, Cat# VPI-1001) as described [15 (link)]. For ocln overexpression, cells were transfected with 1 μg PCMV3-OCLN plasmid (Sino Biological, Wayne, PA, Cat# HG15134-UT) or with PCMV3 (Sino Biological, Cat# CV011) as a negative control. For gene silencing, cells were transfected with 0.5 μg per 106 cells of the following small interfering RNA (siRNA): 3 unique 27mer OAS1-siRNAs (OriGene, Rockville, MD, USA, Cat# SR303266), 3 unique 27mer OAS2-siRNAs (OriGene, Cat# SR303267), 3 unique 27mer OAS3-siRNAs (OriGene, Cat# SR303268), 3 unique 27mer OASL-siRNAs (OriGene, Cat# SR305676), 3 unique 27mer RNaseL-siRNAs (OriGene, Cat# SR304081), 3 unique 27mer occludin-siRNAs (OriGene, Cat# SR303274), 3 unique 27mer TJP1-siRNAs (OriGene, Cat# SR322042), or trilencer-27 universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) as a negative control.
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