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C57bl 6n

Manufactured by Taconic Biosciences
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Sourced in Germany, United States, Denmark

C57BL/6N is a widely used inbred mouse strain that serves as a standard animal model in biomedical research. It is a subline of the C57BL/6 strain, which is one of the most frequently utilized mouse strains. C57BL/6N mice exhibit stable, well-characterized genetic and phenotypic traits, making them a valuable tool for scientific investigations.

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Lab products found in correlation

Swiss webster, by Taconic Biosciences (2 mentions) C57bl 6n, by Charles River Laboratories (2 mentions) 129 svev mice, by Taconic Biosciences (2 mentions) Fvb ntac, by Taconic Biosciences (1 mentions) 129s6 svevtac, by Taconic Biosciences (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Swiss webster mice, by Taconic Biosciences (1 mentions) Male c57bl 6j mice, by Taconic Biosciences (1 mentions) δdblgata1 mice, by Jackson ImmunoResearch (1 mentions) Balb c, by Jackson ImmunoResearch (1 mentions) Metaphor agarose, by Lonza (1 mentions) Ccr5 mice, by Taconic Biosciences (1 mentions) C57bl 6n mice, by Taconic Biosciences (1 mentions) C3h hen, by Taconic Biosciences (1 mentions) Rodent diet 5l0d, by PicoLab (1 mentions) B6 sjl, by Taconic Biosciences (1 mentions) Facsaria iiu, by BD (1 mentions)

26 protocols using c57bl 6n

1

Generation and Characterization of Shank3 Knockout Mice

Cited 2 times
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All animal procedures were approved by the Institutional Animal Care and Use Committee of the Icahn School of Medicine at Mount Sinai and the James J. Peters Veterans Affairs Medical Center (JPVAMC). Shank3-deficient mice were generated using Bruce4 C57BL/6 embryonic stem cells, genotyped as described previously (Bozdagi et al., 2010 (link); Yang et al., 2012 (link)) and maintained on a pure C57BL/6N background (Taconic, Germantown, NY). Two additional lines of mice were generated by backcrossing C57BL/6N animals on FVB/NTac and 129S6/SvEvTac (Taconic) backgrounds using the Taconic Speed Congenics program to achieve >99% congenic animals. For each line, heterozygotes were mated to generate litters that consisted of three genotypes – wild type, heterozygotes and knockout. The mice were weaned at 21 days of age, and one or two littermates from each genotype were group housed in standard plastic cages of three to six littermates per cage. Standard rodent chow and tap water were available ad libitum. The colony room was maintained on a 12 hours light-dark cycle at a constant temperature of 21–22°C and 55% humidity.
Drapeau E., Dorr N.P., Elder G.A, & Buxbaum J.D. (2014). Absence of strong strain effects in behavioral analyses of Shank3-deficient mice. Disease Models & Mechanisms, 7(6), 667-681.
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2

Murine Models for Colitis Studies

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C57Bl6/J (Jackson Lab., USA) wild type mice were used for colitis models with antioxidant administration, while C57Bl6/N (Taconic, Germany) wild type mice between 6 and 8 weeks of age were used for all Lactobacillus colitis studies. Both mouse strains were kept in IVC cages in a specific pathogen free (SPF) facility with maintained temperature (21 °C), humidity (55%), 12 h light/dark cycle and were continuously bred in the same room of the facility. They received ad libitum irradiated chow (Teklad Global) and sterilized filtered water (0.2 µm filter). Experimental groups were randomly assigned by combining age and gender matched littermates of several in-house breeders. Male and female mice were used. All animal experiments were carried out in compliance with EU Directive 86/609/EEC, were approved by the Institutional Animal Research Ethics Committee and were authorized by Irish Regulatory Authority.
Singh A.K., Hertzberger R.Y, & Knaus U.G. (2018). Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis. Redox Biology, 16, 11-20.
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3

Genetically Modified Mouse Lines for RNA-Seq

Cited 1 time
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Aldh1l1-eGFP (MMRRC #3843271) on a Swiss-Webster background were acquired from MMRRC and maintained by breeding with Swiss-Webster mice (from Taconic). Hemizygous transgenic mice and wild-type littermates were used for experiments. B6N.129-Rpl22tm1.1Psam/J (JAX# 011029) were acquired from the Jackson Laboratory and bred with Aldh1l1-cre/ERT2 mice (Srinivasan et al., 2016 (link)) (N3 backcrossed to C57Bl/6N (from Taconic) from an in-house colony; hemizygous transgenic heterozygous knock-in mice were used for RNA-Seq experiments.
Chai H., Diaz-Castro B., Shigetomi E., Monte E., Octeau J.C., Yu X., Cohn W., Rajendran P.S., Vondriska T.M., Whitelegge J.P., Coppola G, & Khakh B.S. (2017). Neural circuit-specialized astrocytes: transcriptomic, proteomic, morphological and functional evidence. Neuron, 95(3), 531-549.e9.
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4

Generating Knockout and Wild-type Mice

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MCT4+/− mice were purchased from Taconic Bioscience. The animals were backcrossed for 10 generations to C57Bl/6N (Taconic) mice and MCT4+/− mice were used for breeding to obtain knock out and wild type littermates. Genotype was confirmed by PCR. Mice were kept in a 12:12 light/dark cycle and provided with ad libitum food and drinking water.
Bisetto S., Whitaker-Menezes D., Wilski N.A., Tuluc M., Curry J., Zhan T., Snyder C.M., Martinez-Outschoorn U.E, & Philp N.J. (2018). Monocarboxylate Transporter 4 (MCT4) Knockout Mice Have Attenuated 4NQO Induced Carcinogenesis; A Role for MCT4 in Driving Oral Squamous Cell Cancer. Frontiers in Oncology, 8, 324.
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5

Ocular P. aeruginosa Infection in Mice

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Mice were housed and bred at the MCP Animal Care Facility. Swiss Webster (SW) and C57BL/6N mice were purchased from Taconic Farms. Seven to nine-week old mice were used throughout the experiments. We have not observed significant differences in the bacterial clearance of male and female mice to ocular P. aeruginosa–induced infection.
Kugadas A., Geddes‐McAlister J., Guy E., DiGiandomenico A., Sykes D.B., Mansour M.K., Mirchev R, & Gadjeva M. (2019). Frontline Science: Employing enzymatic treatment options for management of ocular biofilm‐based infections. Journal of Leukocyte Biology, 105(6), 1099-1110.
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6

Germ-free Mice for Microbiome Research

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Six-week old, female C57BL/6N germ-free mice were purchased from Taconic USA and maintained at a facility in either the University of British Columbia or Joslin Diabetes Center at Harvard Medical School. In each facility, mice were housed in cages with 3 mice per cage and given access to sterilized food and water ad libitum. Mice were allocated to experimental groups randomly. After inoculation, mice were housed in a barrier facility at UBC and a germ-free facility at Joslin, and sterility was maintained for 3 days until the experimental endpoint. All experimental procedures were conducted under protocols approved by the Institutional Animal Care and Use Committee (IACUC) at each institution.
Brown E.M., Ke X., Hitchcock D., Jeanfavre S., Avila-Pacheco J., Nakata T., Arthur T.D., Fornelos N., Heim C., Franzosa E.A., Watson N., Huttenhower C., Haiser H.J., Dillow G., Graham D.B., Finlay B.B., Kostic A.D., Porter J.A., Vlamakis H., Clish C.B, & Xavier R.J. (2019). Bacteroides-derived sphingolipids are critical for maintaining intestinal homeostasis and symbiosis. Cell host & microbe, 25(5), 668-680.e7.
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7

Husbandry of C57BL/6 Male Mice

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10 week-old C57BL/6N and C57BL/6J male mice were respectively purchased from Taconic (Germantown, NY) and The Jackson Laboratories (Bar Harbor, ME). Food and water were freely available throughout the experiments. Mice were housed individually in open cages placed in ventilated housing enclosures with a controlled light cycle.
Combe R., Mudgett J., El Fertak L., Champy M.F., Ayme-Dietrich E., Petit-Demoulière B., Sorg T., Herault Y., Madwed J.B, & Monassier L. (2016). How Does Circadian Rhythm Impact Salt Sensitivity of Blood Pressure in Mice? A Study in Two Close C57Bl/6 Substrains. PLoS ONE, 11(4), e0153472.
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8

Breeding and Maintaining Mouse Strains

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Breeders of IL-33fl/fl-eGFP reporter (cat#030619), Balb/c (cat#000651), and ΔdblGata1 mice (cat#005653) were purchased from the Jackson laboratory. Breeders of IL-33−/− mice (on C57BL/6N genetic background) were obtained from J. Brown (University of Colorado Anschutz Medical Campus). C57BL/6N from Taconic Biosciences were used as WT control mice. All colonies were established and maintained in the UTHealth animal facility. All experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee at UTHealth.
Xu L., Yang Y., Wen Y., Jeong J.M., Emontzpohl C., Atkins C.L., Sun Z., Poulsen K.L., Hall D.R., Bynon J.S., Gao B., Lee W.M., Rule J., Jacobsen E.A., Wang H, & Ju C. (2022). Hepatic recruitment of eosinophils and their protective function during acute liver injury. Journal of hepatology, 77(2), 344-352.
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9

Generation and Characterization of Cyp2c Knockout Mice

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Animal experiments were conducted in wild-type (WT) and Cyp2c-knockout (Cyp2c−/−) mice with C57BL/6 N background obtained from Taconic Biosciences (New York, NY, USA). The mice (model # 9177) were originally generated by a Cre-mediated germline deletion of 14 out of 15 isoforms of the Cyp2c genes. The deleted genes, which are located within a 1.2 Mb cluster on chromosome 19, include Cyp2c55, 2c65, 2c66, 2c29, 2c38, 2c39, 2c67, 2c68, 2c40, 2c69, 2c37, 2c54, 2c50, and 2c70. Cyp2c44, which is physically located outside of this cluster, has not been deleted. The resultant chimeras were backcrossed to C57BL/6 N WT mice [22 (link)]. Male and female heterozygotes were subsequently purchased from Taconic Biosciences and bred to generate knockout mice and littermate controls for this study. Mice were genotyped using a genotyping kit (#KK5621, KAPA Biosystems). The DNA products after PCR were resolved by electrophoresis on a 2% agarose gel with 0.05% ethidium bromide. The primer sequences used for genotyping are provided in Supplemental Table 1.
Oteng A.B., Higuchi S., Banks A.S, & Haeusler R.A. (2021). Cyp2c-deficiency depletes muricholic acids and protects against high-fat diet-induced obesity in male mice but promotes liver damage. Molecular Metabolism, 53, 101326.
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10

Generation and characterization of Reln(CTRdel) mice

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Generation and characterization of RelnCTRdel mice (RRID: MGI:5505412) was previously described [19 (link),26 (link)]. The mutant mice have a mixed background, carrying A/J alleles in proximal chromosome 5~28 Mb region flanked by microsatellite markers D5Mit193 and D5Mit386. The mice analyzed were outcrossed at least eight generations to C57BL6/N (Taconic). Mice of both sexes were analyzed. Animals were maintained in accordance with guidelines of National Institutes of Health and the Seattle Children’s Hospital Institutional Animal Care and Use Committee.
Ha S., Tripathi P.P., Daza R.A., Hevner R.F, & Beier D.R. (2020). Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis. Journal of Developmental Biology, 8(3), 20.
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