Optima lc ms grade methanol

Manufactured by Thermo Fisher Scientific

Sourced in United States, Canada, Germany, New Zealand

Optima LC-MS grade methanol is a high-purity solvent designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. It is produced to meet the stringent requirements of LC-MS analysis, ensuring minimal interference and optimized performance.

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Methanol (2986 citations) LC-MS grade methanol (172 citations) Optima grade (92 citations) LC-MS grade (75 citations) Optima LC/MS (67 citations) MS-grade methanol (30 citations) Optima grade methanol (29 citations) Methanol (LC grade) (7 citations) Methanol Optima® (6 citations) LC/MS methanol (4 citations)

46 protocols using optima lc ms grade methanol

Quantification of DNA Adducts by UHPLC

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Milli-Q ultra-pure water (Merck Life Sciences, Søborg, Denmark), methanol optima LC/MS grade from Thermo Fisher Scientific (Waltham, MA), and ammonium bicarbonate from Merck (St. Louis, MO.) were used for the UHPLC analysis. The following DNA adducts reference standards, were purchased from Toronto Research Chemicals: 2′-deoxy-N⁶-methyladenosine (N⁶-Me-dA); 5-methyl-2′-deoxycytidine (5-Me-dC); O⁶-methyl-2′-deoxyguanosine (O⁶-Me-dG); N3-methylthymidine (3-Me-dT); N⁴,5-dimethyldeoxycytidine (N⁴,5-DiMe-dC); N²-ethyl-2′-deoxyguanosine (N²-ethyl-dG); N⁶-(2-hydroxyethyl)-2′-deoxyadenosine (N⁶-(2-OH-ethyl)-dA); 8-oxo-2′deoxyguanosine (8-oxo-dG); etheno-2′-deoxy-β-D-adenosine (1,N⁶-ε-dA); 3,N⁴-etheno-2′-deoxycytidine (3,N⁴-ε-dC); 3-(2-deoxy-β-D-erythro-pentofuranosyl)pyrimido [1,2-a]purin-10(3H)-one (M1-dG); 3-(2-Deoxy-β-D-erythro-pentofuranosyl)-3,5-dihydropyrimido [1,2-a]purine-6,10-dione (6-Oxo-M1-dG); γ-Hydroxy-1,N2-propano-2′-deoxyguanosine (1,N²-γ-OH-P-dG) (Acr-1I-dG); N-(2′-deoxyguanosin-8-yl)-4-aminobiphenyl (8-ABP-dG); and N2-(deoxyguanosin-8-yl)-2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (8-MeIQx-dG). Stock solutions of the DNA adduct standards were dissolved at 1 or 0.5 mg ml⁻¹ in methanol, or a mixture of water and methanol. The working solutions were diluted with water to 100 ng ml⁻¹.

La Barbera G., Nommesen K.D., Cuparencu C., Stanstrup J, & Dragsted L.O. (2022). A Comprehensive Database for DNA Adductomics. Frontiers in Chemistry, 10, 908572.

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Fluralaner Extraction and UPLC/MS Analysis

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Technical grade fluralaner was purchased from Cayman Chemical (Ann Arbor, MI, USA) and used as a standard in developing and validating the extraction and UPLC/MS procedures. Acetonitrile Optima LC–MS grade, methanol Optima LC–MS grade, formic acid Optima LC–MS grade, and o-phosphoric acid (85wt%) HPLC grade, were purchased from Fisher Scientific (Thermo Fisher, Waltham, MA, USA). Ultrapure (type 1) water was obtained from a Millipore Synergy UV water purification system (Millipore Sigma, Burlington, MA, USA).

González-Morales M.A., Thomson A.E., Yeatts J., Enomoto H., Haija A., Santangelo R.G., Petritz O.A., Crespo R., Schal C, & Baynes R. (2023). Pharmacokinetics of fluralaner as a systemic drug to control infestations of the common bed bug, Cimex lectularius, in poultry facilities. Parasites & Vectors, 16, 333.

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Synthesis and Characterization of Potassium Ferrate

Cited 1 time

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Ultrapure water (18.2 MΩ•cm) was obtained from a Milli-Q Element A10 water purification system. Suwannee River NOM (SRNOM, 2R101N) and Suwannee River humic acid (SRHA, 3S101H) were purchased from the International Humic Substances Society. Solid potassium ferrate (K₂FeO₄) was chemically prepared based on previous studies (Yang et al., 2015 ; Dong et al., 2019 (link)). Stock solutions of Fe(VI) were freshly prepared by dissolving K₂FeO₄ in ultrapure water before each experiment, and Fe(VI) concentration was quantified with the absorbance at 510 nm (absorption coefficient 1150 M⁻¹ cm⁻¹). HF Mega Bond Elut C18 cartridges (1 g, 6 mL) were ordered from Agilent Technologies. Lead(IV) oxide (> 97%, extra pure, ACROS Organics) were ordered from Fisher Chemical (Hampton, NH, USA), and it has been identified as pure plattnerite (β-PbO₂) by X-ray Diffraction in a previous study (Xie et al., 2010 (link)). Other chemicals and reagents, including potassium iodide (KI, 99+%, ACS reagent), potassium bromide (KBr, 99+%, for spectroscopy, IR grade), nitric acid (trace metal grade), and methanol (Optima LC/MS grade) were purchased from Fisher Chemical (Hampton, NH, USA).

Liu J., Mulenos M.R., Hockaday W.C., Sayes C.M, & Sharma V.K. (2020). Ferrate(VI) pretreatment of water containing natural organic matter, bromide, and iodide: A potential strategy to control soluble lead release from PbO2(s). Chemosphere, 263, 128035.

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Newborn Screening for Galactosemia

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The NeoBase non-derivatized newborn screening kit and the NeoBase succinylacetone assay solution were purchased from Perkin Elmer, Waltham, MA, USA. Formic acid Ph Eur 98–100% was purchased from Merck, Darmstadt, Germany. Methanol Optima LC/MS grade was purchased from Fisher Scientific, Waltham, MA, USA. Galactose-1-phosphate ≥98% was purchase from Sigma Aldrich, Copenhagen, Denmark. Galactose-1-phosphate-13C6 98.4% was purchase from Omnicron Biochemicals, South Bend, IN, USA. The extraction solution was prepared according to NeoBase kit insert (including the succinylacetone assay solution) with the following modification. An isotopically labelled GAL-1-P was added to achieve a final solution of 0.15 mmol/L. The enzyme activity assay Neonatal GALT was purchased from Perkin Elmer.

Cohen A.S., Baurek M., Lund A.M., Dunø M, & Hougaard D.M. (2019). Including Classical Galactosaemia in the Expanded Newborn Screening Panel Using Tandem Mass Spectrometry for Galactose-1-Phosphate. International Journal of Neonatal Screening, 5(2), 19.

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Certified Reference Materials for Paralytic Shellfish Toxins

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Certified reference materials for C1/2, GTX1/4, GTX2/3, dcGTX2/3, GTX5, GTX6, STX, NEO, dcSTX, dcNEO, an in-house reference material of C3/4 were obtained from the National Research Council Canada (Halifax, Nova Scotia, Canada). Formic acid and ammonium formate were obtained from Sigma-Aldrich (Oakville, ON, Canada). Acetic acid (AcOH) and hydrochloric acid were purchased from Caledon (Georgetown, ON, Canada). Acetonitrile and methanol (Optima LC-MS grade) were purchased from Fisher Scientific (Mississauga, ON, Canada). Deionized water (18.2 MΩ cm) was obtained from a Milli-Q gradient A10 purification system (Millipore Corp., Billerica, MA, USA).

Qiu J., Wright E.J., Thomas K., Li A., McCarron P, & Beach D.G. (2020). Semiquantitation of Paralytic Shellfish Toxins by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry Using Relative Molar Response Factors. Toxins, 12(6), 398.

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Quantification of Phenolic Compounds

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Folin–Ciocalteu phenol reagent was purchased from Sigma-Aldrich (St. Louis, MO, USA), while sodium carbonate anhydrous was from Merck KGaA (Darmstadt, Germany). Deionized water was used throughout and obtained using a Milli-Q_PLUS system (Merck). Ethanol absolute, HPLC grade, Sharlau (Barcelona, Spain) and methanol for UV, IR, HPLC, ACS, PanReac Applichem (Darmstadt, Germany) were used for the extractions, while methanol OPTIMA LC/MS grade, Fisher Chemical (Madrid, Spain) was used for HPLC analysis. Catechin, chlorogenic acid, quercetin, p-coumaric acid, gallic acid, and phloridzin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The individual stock solutions of phenolic compounds were prepared in MeOH:H₂O (70:30, v/v) at 1000 µg/mL and maintained at −20 °C in the dark. Matrix-matched calibration curves at concentrations between 0.05 and 21 µg/mL were used to quantify the phenols in samples.

Pollini L., Cossignani L., Juan C, & Mañes J. (2021). Extraction of Phenolic Compounds from Fresh Apple Pomace by Different Non-Conventional Techniques. Molecules, 26(14), 4272.

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Protein Standards Preparation and Characterization

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Protein standards: ubiquitin from bovine erythrocytes, cytochrome c and myoglobin from equine heart were obtained as lyophilized powders (Sigma Aldrich). All standards were reconstituted as received in high purity water and stored at ca. -4 °C before further dilution. Initial stock solutions of 1 mg/ml of all proteins were prepared in 18 MΩ deionized water (Milli-Q, Millipore). Myoglobin sample was in addition dialyzed overnight (Slider-A-Lyzer Dialysis Cassettes) to remove excess salts. Two sets of analytical stock solutions at ca. 1 ug/mL were prepared in aqueous buffered (high pH) and organic acidified (low pH) solution conditions. All buffered samples were prepared in 20 mM ammonium acetate (77.08 g/mol, ≥ 99% purity, Fisher Scientific), whereas samples in the organic solvents were prepared in 50 % methanol/50 % water with 0.2 % formic acid. High purity water, methanol (optima LC-MS grade, Fisher Scientific) and formic acid (≥ 99% purity, VMD International Ltd.) were used as received from the vendors. The pH of each prepared sample solution was measured using a pH meter (SevenEasy, Mettler-Toledo, Columbus, OH), and corresponded to a pH range between 2.7 and 3.8 for acidic organic samples and a pH range between 6.5 and 6.7 for aqueous buffered samples. The pH values were measured after all experiments to minimize contamination due to sample carryover.

May J.C., Jurneczko E., Stow S.M., Kratochvil I., Kalkhof S, & McLean J.A. (2017). Conformational Landscapes of Ubiquitin, Cytochrome c, and Myoglobin: Uniform Field Ion Mobility Measurements in Helium and Nitrogen Drift Gas. International journal of mass spectrometry, 427, 79-90.

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Quantification of Fluralaner by LC-MS/MS

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Fluralaner was purchased from Cayman chemical (Ann Arbor, MI). Acetonitrile Optima LC-MS grade (ACN), Methanol Optima LC-MS grade (MeOH), Formic acid Optima LC-MS grade, and o-phosphoric acid (85wt%) HPLC grade, were purchased from Fisher Scientific (Thermo Fisher, Waltham, MA). Ultrapure (type 1) water was obtained from a Millipore Synergy UV water purification system (Millipore Sigma, Burlington, MA) in the lab. Physicochemical properties of Fluralaner are shown in Table 1.Table 1

Physicochemical properties and structure of Fluralaner.

Table 1

Molecular formula	C₂₂H₁₇C_l2F₆N₃O₃
Molecular weight	556.3 g/mol
Log P	5.4
Water solubility	0.1 mg/L

Elliot B.A., Enomoto H., Petritz O., Crespo R., Yeatts J., Fricke I., Singleton A., Thomson A, & Baynes R.E. (2023). Pharmacokinetics of intravenously and trans-dermally administered fluralaner in healthy laying shaver hens: fluralaner in chickens. Poultry Science, 103(3), 103362.

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Oxylipin Extraction and Quantification

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Acetic acid and methanol (Optima LC/MS Grade) as well as acetonitrile (HPLC-MS grade) were obtained from Fisher Scientific (Schwerte, Germany) and ammonium acetate (p.a.) was purchased from Merck (Darmstadt, Germany). Methyl tert-butyl ether and n-hexane (HPLC grade) were obtained from Carl Roth (Karlsruhe, Germany). Methyl tricosanoate (FAME C23:0) was obtained from Santa Cruz Biotechnology (Heidelberg, Germany). Oxylipin and deuterated oxylipin standards were purchased from Cayman Chemicals (local distributor: Biomol, Hamburg, Germany). Further oxylipin standards (Epoxy octadecadienoic acids (EpODEs) and dihydroxy octadecadienoic acids (DiHODEs)) were a kind gift from the laboratory of Bruce Hammock (UC Davis, CA, USA). Ethyl acetate, methyl formate and all other chemicals were purchased from Sigma Aldrich (Taufkirchen, Germany).

Ostermann A.I., Waindok P., Schmidt M.J., Chiu C.Y., Smyl C., Rohwer N., Weylandt K.H, & Schebb N.H. (2017). Modulation of the endogenous omega-3 fatty acid and oxylipin profile in vivo—A comparison of the fat-1 transgenic mouse with C57BL/6 wildtype mice on an omega-3 fatty acid enriched diet. PLoS ONE, 12(9), e0184470.

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Quantification of 3-Hydroxy and 3-Oxopentanoic Acids

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Analytical standards, 3-hydroxypentanoic acid (purity 95%; Fig. 1a), 3-oxopentanoic acid (purity 97%; Fig. 1b) were purchased from Toronto Research Chemicals (Toronto, ON, Canada) and sulbactam (Internal Standard (IS); purity ≥ 99.7%; Fig. 1c) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Methanol (Optima LC-MS grade), water (Optima LC-MS grade), and formic acid (Optima LC-MS grade) were purchased from Fisher Chemicals (Hampton, NH, USA). Human plasma with potassium ethylenediaminetetraacetic acid (K₂EDTA) as the anticoagulant was procured from BioIVT (Westbury, NY, USA). All chemicals and reagents used without further purification.

Kallem R.R., Pascual J.M., Primeaux S., Avila A, & Putnam W.C. (2021). Development and validation of a LC-MS/MS method for quantitation of 3-hydroxypentanoic acid and 3-oxopentanoic acid in human plasma and its application to a clinical study of glucose transporter type I deficiency (G1D) syndrome. Journal of pharmaceutical and biomedical analysis, 205, 114335.

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