The mouse kidneys were perfused with 10% formalin in 9 mmol/l sodium cacodylate, 0.05% picric acid, and 135 mmol/l sucrose, and fixed in 4% paraformaldehyde and 2.5% glutaraldehyde in PBS on ice for 2 h. For scanning electron microscopy, the samples were treated with 2% OsO4 in PBS for 2 h, dehydrated in a graded series of ethanol (from 60% to 100%) at room temperature, and soaked into 3-methylbutylacetate to be dried completely in Critical Point Dryer (Hitachi). The dried specimens were mounted on brass stages and coated with ionized gold particles (20 nm) in Ion Corter IB-3 (Eiko Engineering). The samples were observed in a JSM-7505FA electron microscope (JOEL Ltd) at an accelerating voltage of 10 kV. For transmission electron microscopy, the samples were washed with PBS, treated with 2% OsO4 in PBS on ice for 2 h, dehydrated in graded ethanol, and embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate for 2 h and lead nitrate for 3 min. The samples were observed with a Hitachi H-7500 electron microscope (Hitachi High-Tech).
H 7500 electron microscope
Manufactured by Hitachi
Sourced in Japan
The H-7500 is a high-resolution transmission electron microscope (TEM) manufactured by Hitachi. It is designed to provide detailed imaging and analysis of samples at the nanoscale level. The H-7500 operates with an accelerating voltage of up to 100 kV and offers a maximum magnification of up to 600,000x. It is equipped with advanced imaging and analysis capabilities to support a wide range of applications in materials science, life sciences, and other fields.
Automatically generated - may contain errors
Lab products found in correlation
Sc1000, by Ametek (2 mentions)
Quetol 812, by Nisshin EM (2 mentions)
Reichert ultracut e, by Leica (2 mentions)
Critical point dryer, by Hitachi (1 mentions)
Ultracut r, by Leica (1 mentions)
Ultramicrotome, by Leica (1 mentions)
Polyethylenimine (pei), by Polysciences (1 mentions)
S 4700 microscope, by Hitachi (1 mentions)
Glutaraldehyde, by Nacalai Tesque (1 mentions)
Em uc6 ultramicrotome, by Leica (1 mentions)
Epon 812 resin mixture, by TAAB (1 mentions)
Ultracut s ultramicrotome, by Leica (1 mentions)
Eponate 12, by Ted Pella (1 mentions)
Durcupan acm, by Merck Group (1 mentions)
Rabbit anti c peptide, by Cell Signaling Technology (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Epon812 medium grade resin, by Polysciences (1 mentions)
Uv 3600 spectrophotometer, by Shimadzu (1 mentions)
Propylenoid, by Ladd Research Industries (1 mentions)
Lx 112, by Ladd Research Industries (1 mentions)
66 protocols using h 7500 electron microscope
1
Ultrastructural Analysis of Mouse Kidneys
2
Histological and Ultrastructural Analysis of X-SCID Rat Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were euthanized under isoflurane anesthesia. Tissues collected from 1- to 8.5-month-old X-SCID rats (n=23) were fixed in 10% neutral buffered formalin, and embedded in paraffin. The
following tissues were sampled: liver, spleen, kidneys, heart, lungs, thymus, salivary glands, exorbital lacrimal glands, Harderian glands, testes, deferent ducts, epididymis, prostate
glands, uterus, ovaries, and oviducts. Four µm-thick sections were cut and stained with hematoxylin and eosin (HE). Formalin-fixed tissues were also stored in 2.5%
glutaraldehyde in 0.1 M phosphate buffer (PB; pH 7.4), post-fixed with 1% osmic tetraoxide at 4°C overnight, and embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate
and lead citrate, and examined in a Hitachi H-7500 electron microscope (Hitachi, Tokyo, Japan).
following tissues were sampled: liver, spleen, kidneys, heart, lungs, thymus, salivary glands, exorbital lacrimal glands, Harderian glands, testes, deferent ducts, epididymis, prostate
glands, uterus, ovaries, and oviducts. Four µm-thick sections were cut and stained with hematoxylin and eosin (HE). Formalin-fixed tissues were also stored in 2.5%
glutaraldehyde in 0.1 M phosphate buffer (PB; pH 7.4), post-fixed with 1% osmic tetraoxide at 4°C overnight, and embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate
and lead citrate, and examined in a Hitachi H-7500 electron microscope (Hitachi, Tokyo, Japan).
3
Histopathological Analysis of Spinal Cord in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats (3, 5, 7, 8, 9, and 10 weeks of age) were euthanized under isoflurane anesthesia. We examined a total 112 rats for histopathology (homozygous; n = 79 and WT; n = 33). Tissue samples from the central nervous system (CNS) were fixed in 10% neutral buffered formalin, and embedded in paraffin. Sections of 4 μm thick were cut and stained with hematoxylin and eosin (HE). We also counted the number of spheroids (exceedingly 5 μm in diameter) in the transverse sections of the cervical (C5 level) and lumber (L1–2 level) spinal cord at 10 weeks of age by microscopic observation. The areas of 2.37 mm2 (high-power fileld) in the dorsal cord of the spinal cord from five different animals were evaluated in each experimental group. The data are presented as the number of spheroids/mm2. For transmission electron microscopy (TEM), two formalin-fixed tissues of kk-homozygous rats at 5 and 10 weeks of age were stored in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4), post-fixed with 1% osmium tetraoxide at 4°C overnight and embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with a Hitachi H-7500 electron microscope (Hitachi, Tokyo, Japan).
4
Ultrastructural Analysis of Trigeminal Ganglion Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On day 22 after surgery, when mechanical allodynia is most prominent, rats of the sham-operated and CCI-ION groups, as well as naïve rats, were deeply anesthetized with sodium pentobarbital (80 mg/kg, i.p.) and perfused intracardially with 100 mL heparinized 0.9% saline, followed by 500 mL freshly-prepared fixative, containing 1% PFA and 2.5% glutaraldehyde in 0.1 M PB. TGs were dissected out and fixed in the fixative used for perfusion for an additional 2 h at 4°C. Sections were cut at 50 μm on a Vibratome and osmicated in 1% osmium tetroxide in PB for 30 min. After that, sections were dehydrated in graded ethanol, flat-embedded in Durcupan ACM (Fluka, Buchs, Switzerland) between Aclar plastic films (EMS, Hatfield, PA), and cured for 48 h at 60°C. Small chips containing the ophthalmo-maxillary region of the TG were cut out of the wafers and glued onto blank resin blocks with cyanoacrylate. Thin sections were cut with a diamond knife, mounted on formvar-coated single slot nickel grids, and stained with uranyl acetate and lead citrate. Grids were examined on a Hitachi H7500 electron microscope (Hitachi, Tokyo, Japan) at 80 kV accelerating voltage. Images were captured with Digital Micrograph software driving a cooled CCD camera (SC1000; Gatan, Pleasanton, CA) attached to the microscope, and saved as TIFF files.
5
Glutaraldehyde Fixation and Electron Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The anterior walls of the left ventricle were fixed in 2.5% glutaraldehyde and 1% paraformaldehyde at four °C for 24 h and then washed with 0.1 M phosphate-buffered solution at 4 °C, stained in 2% osmium tetroxide, embedded and sectioned. After washing twice, the tissues were postfixed with a 1% OsO4-buffered solution (pH 7.4) for 1 h. After being dehydrated and embedded, the resins were sectioned using an EM Ultramicrotome LKB-2088 and stained with 1% toluidine blue. The ultrathin sections were then double-stained with uranyl acetate and lead citrate and examined with a Hitachi H-7500 electron microscope (Hitachi, Tokyo, Japan).
6
Apoptosis Evaluation by Flow Cytometry and Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This assay involved staining cells with Annexin V-FITC (a phospholipid-binding protein binding to disrupted cell membranes) in combination with PI (a vital dye binding to DNA penetrating into apoptotic cells). Flow cytometry (FACS) was performed for determining the percentage of apoptotic cells (Annexin V + /PI).
Electron microscopy. After collection, NB4 cells were fixed in 2.5% glutaraldelhyde for at least 3 h and subsequently treated with 2% paraformaldehyde at room temperature for 60 min, 0.1% glutaraldehyde in 0.1 M sodium cacodylate for 2 h and post-fixation with 1% osmium tetroxide (OsO 4 ) for 1.5 h. After the second rinsing, the samples were dehydrated with graded acetone and ultimately embedded in Quetol 812. Ultrathin sections were observed under Hitachi H7500 electron microscope (Hitachi, Tokyo, Japan).
Caspase activity assay. Caspase-3 activity was assayed by caspase-3 colorimetric assay kit (Calbiochem, Berlin, Germany) according to the manufacturer's instructions.
Statistical analysis. The quantitative data were presented as means ± standard deviation. In addition, analysis was performed with specified statistical methods using GraphPad Prism (version 5.04). For calculating P-value, two-tailed parameters with a confidence interval of 95% were used. A P-value <0.05 was considered to indicate a statistically significant result.
Electron microscopy. After collection, NB4 cells were fixed in 2.5% glutaraldelhyde for at least 3 h and subsequently treated with 2% paraformaldehyde at room temperature for 60 min, 0.1% glutaraldehyde in 0.1 M sodium cacodylate for 2 h and post-fixation with 1% osmium tetroxide (OsO 4 ) for 1.5 h. After the second rinsing, the samples were dehydrated with graded acetone and ultimately embedded in Quetol 812. Ultrathin sections were observed under Hitachi H7500 electron microscope (Hitachi, Tokyo, Japan).
Caspase activity assay. Caspase-3 activity was assayed by caspase-3 colorimetric assay kit (Calbiochem, Berlin, Germany) according to the manufacturer's instructions.
Statistical analysis. The quantitative data were presented as means ± standard deviation. In addition, analysis was performed with specified statistical methods using GraphPad Prism (version 5.04). For calculating P-value, two-tailed parameters with a confidence interval of 95% were used. A P-value <0.05 was considered to indicate a statistically significant result.
7
Ultrastructural Analysis of Lipid Droplets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4% glutaraldehyde prior to postfixation in 1% osmium tetroxide. Samples were processed and embedded in Epon-Araldite resin. Ultrathin sections were obtained by using an ultramicrotome and counterstained with lead citrate. A Hitachi H-7500 electron microscope was used to observe lipid droplets and autophagosomes.
8
Transmission Electron Microscopy of EVs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Resuspended HPA‐derived EVs from the 2000, 10,000, 100,000, and 167,000 × g were deposited on 200‐mesh Formvar‐coated copper grids and the membranes were covered for 4–5 min for the absorption. Next, for contrast staining, the grids were further transferred to uranyl acetate solution and washed with PBS, and allowed to air‐dry at room temperature. Imaging was performed using a Hitachi H‐7500 electron microscope (Hitachi, Tokyo, Japan) at 200 kV.
9
Ultrastructural Analysis of HCT-116 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCT-116 cells were administered DMSO or oxaliplatin in combination with PL after pretreatment or not with 5 mM NAC for indicated times. After treatment, cells underwent fixation with 2.5% glutaraldehyde in PBS overnight at 4 °C and postfixation with 1% OsO4 in ambient conditions for 1 h. Following the fixation procedure, cells were stained with 1% uranyl acetate, and underwent dehydration (graded acetone solutions) and Epon embedding. Finally, 70 nm sections were obtained and assessed under an H-7500 electron microscope (Hitachi, Japan).
10
Ultrastructural Analysis of Explants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The explants at culture day 14 were fixed with cacodylate-buffered 2% glutaraldehyde solution for 24 h, and, after rinsing, post-fixed with 1% osmium tetroxide. The explants were stained en bloc with uranyl acetate to preserve lamellar inclusion bodies [29 ], dehydrated with a graded ethanol, and embedded in Epon–Araldite mixture. Thick sections were stained with 1% toluidine blue before making ultra-thin sections. The ultra-thin sections were examined with a Hitachi H7500 electron microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!