Ex527

Manufactured by MedChemExpress

Sourced in United States, China

EX527 is a lab equipment product from MedChemExpress. It is a selective and potent inhibitor of the protein SIRT1.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (11 mentions) Compound c, by MedChemExpress (4 mentions) Lipopolysaccharides (lps), by Merck Group (3 mentions) Srt1720, by MedChemExpress (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dorsomorphin, by MedChemExpress (2 mentions) Sr 18292, by MedChemExpress (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Palmitate pa, by Merck Group (1 mentions) Thapsigargin thp, by Merck Group (1 mentions) Resveratrol, by Merck Group (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) Mab4470, by R&D Systems (1 mentions) Atrogin 1 ab168372, by Abcam (1 mentions) Gapdh, by Proteintech (1 mentions) Caski, by Merck Group (1 mentions) Sw756, by Merck Group (1 mentions) Lipofectamine 3000 kit, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)

35 protocols using ex527

Puerarin Attenuates LPS-Induced HUVEC Inflammation

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HUVECs were purchased from the Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China). LPS was acquired from Sigma-Aldrich (St. Louis, MO, USA). SRT1720 and EX-527 were purchased from MedChemExpress (Princeton, NJ, USA). Pue (purity ≥ 98%) was purchased from the Chinese Medicine Resource Center, Chinese Academy of Traditional Chinese Medicine (Beijing, China).
The cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, Carlsbad, USA) containing 10% fetal bovine serum (Gibco) and 100 μg/mL penicillin-streptomycin (Gibco). Cells were passaged using trypsin-EDTA (Gibco). The cells were incubated at 37°C, 95% humidity, and 5% CO₂. The medium was renewed every 2 days. HUVECs were used up to passage five [62 (link)]. The HUVECs were activated with 10 μg/mL LPS for 24 h [63 (link)] and pretreated with 10, 20, 50, 100, or 150 mg/L Pue for 24 h before LPS induction. As indicated, HUVECs were incubated with EX-527 (MedChemExpress) for 6 h to inhibit SIRT-1 activity or with SRT1720 (MedChemExpress) for 6 h to activate SIRT-1.

Chang X., Zhang T., Liu D., Meng Q., Yan P., Luo D., Wang X, & Zhou X. (2021). Puerarin Attenuates LPS-Induced Inflammatory Responses and Oxidative Stress Injury in Human Umbilical Vein Endothelial Cells through Mitochondrial Quality Control. Oxidative Medicine and Cellular Longevity, 2021, 6659240.

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Resveratrol Attenuates 3-NPA-Induced Neurotoxicity

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Forty six-week-old female C57BL/6 mice were randomly divided into 5 groups, each with 8 mice: (a) Control group: mice received 0.5% methyl cellulose orally and received intraperitoneal injections of saline; (b) Res group: mice received 40 mg/kg Res (MedChemExpress) orally and received intraperitoneal injections of saline [27 (link)]; (c) 3-NPA group: mice received 0.5% methyl cellulose orally and received intraperitoneal injections of 40 mg/kg 3-NPA; (d) 3-NPA+Res group: mice received 40 mg/kg Res orally and received intraperitoneal injections of 40 mg/kg 3-NPA; (e) Ex-527 group: mice received 0.5% methyl cellulose orally and received intraperitoneal injections of 2.5 mg/kg Ex-527 (MedChemExpress) [28 (link)]. All groups received the same volume of treatment agents for 21 days.

Guo L., Liu X., Chen H., Wang W., Gu C, & Li B. (2022). Decrease in ovarian reserve through the inhibition of SIRT1-mediated oxidative phosphorylation. Aging (Albany NY), 14(5), 2335-2347.

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Investigating RUNX3 and EZH2 Regulation

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Cells in logarithmic growth phase were transfected with siNC, siRUNX3, siNC and siEZH2 for 24 h by using Lipofectamine 3000. At 24 h after transfection, SiEZH2 + EX527 groups were treated with EX527 (10 μM; MedChemExpress) for 24 h.

Liu H., Yan G., Li L., Wang D., Wang Y., Jin S., Jin Z., Li L, & Zhu L. (2022). RUNX3 mediates keloid fibroblast proliferation through deacetylation of EZH2 by SIRT1. BMC Molecular and Cell Biology, 23, 52.

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Isolation and Culture of Primary Mouse Hepatocytes

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Primary hepatocytes were isolated from 8-12-week-old C57BL6/J mice (20-25 g) using a two-step perfusion method, and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), as described previously (26 (link)). For pharmacological studies, cells were treated with 40 U/ml EPO, 0.3 mmol/l palmitate (PA; Sigma-Aldrich; Merck KGaA) or 1 µmol/l thapsigargin (Thp; Sigma-Aldrich; Merck KGaA) for 24 h. Where indicated, the cells were pre-treated with 40 µmol/l resveratrol (Sigma-Aldrich; Merck KGaA) or 2 µmol/l EX-527 (MedChem Express, LLC) for 1 h prior to EPO treatment. Primary hepatocytes isolated from EPO-treated and control mice were incubated with 1, 2 and 10 nmol/l human recombinant FGF21 (Abcam) for 4 h; vehicle treatment served as control.

Hong T., Ge Z., Zhang B., Meng R., Zhu D, & Bi Y. (2019). Erythropoietin suppresses hepatic steatosis and obesity by inhibiting endoplasmic reticulum stress and upregulating fibroblast growth factor 21. International Journal of Molecular Medicine, 44(2), 469-478.

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Molecular Mechanisms of Muscle Atrophy

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UA and Ex-527 were acquired from MedChemExpress (Princeton, NJ, USA). The primary antibody against myosin heavy chain (MyHC, MAB4470) was acquired from R&D Systems (Minneapolis, MN, USA). The primary antibodies against STAT3 (#9139), p-STAT3 (Yyr705) (#9145), NF-κB (p65) (#8242), p-NF-κB (p-p65) (Ser536) (#3033), and SIRT1 (#9475) were acquired from Cell Signaling Technology (Beverly, MA, USA); FOXO3a (#10849-1-Ap), FOXO1 (#18592-1-Ap), NOX4 (#14347-1-Ap), and GAPDH (#10494-1-Ap) were acquired from Proteintech (Wuhan, China); and Atrogin-1 (ab168372) and MuRF1 (ab172479) were acquired from Abcam (Cambridge, MA, USA).

Tao W., Ouyang Z., Liao Z., Li L., Zhang Y., Gao J., Ma L, & Yu S. (2023). Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1. Cancers, 15(8), 2378.

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Sirt1 Inhibition in C2C12 Myotubes

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The SIRT1 inhibitor EX-527 (Med Chem Express) was used to inhibit the expression of SIRT1. C2C12 myotube cells were treated with PA and Liraglutide or semaglutide for 24 hours with or without EX-527.

Xiang J., Qin L., Zhong J., Xia N, & Liang Y. (2023). GLP-1RA Liraglutide and Semaglutide Improves Obesity-Induced Muscle Atrophy via SIRT1 Pathway. Diabetes, Metabolic Syndrome and Obesity, 16, 2433-2446.

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Cervical Cancer Cell Line Manipulation

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Human CC cell lines CaSki, SiHa, C-33A, SW756, and HeLa, and a human immortalized cervical epithelial cell-line End1/E6E7 were procured from American Type Culture Collection (Manassas, VA, USA). The C-33A, SW756, and HeLa cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). CaSki cells were incubated in Roswell Park Memorial Institute-1640 (Sigma-Aldrich). All media contained 10% inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Jiangsu, China). The culture condition was 37°C with 5% CO_2.Small interfering (si) RNA of TCF3 (si-TCF3), overexpression plasmid of SIRT1 (SIRT1-OE) and the negative control (NC) plasmids were provided by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The plasmids were transfected into cells using a Lipofectamine 3000 kit (Thermo Fisher Scientific) at a ratio of 1:2.5 (RNA: Lipofectamine 3000). After 48 h, the cells were treated with 10 μM EX527 (MedChemExpress, Co., Ltd., NJ, USA), a SIRT1 inhibitor, for 24 h to suppress SIRT1 activity, or treated with a β-catenin inhibitor XAV-939 (2 μM, MedChemExpress) for 24 h to suppress β-catenin accumulation in nucleus. Cells treated with dimethyl sulfoxide (DMSO) were set to controls.

Yu X., Li Z., Bai R, & Tang F. (2022). Transcriptional factor 3 binds to sirtuin 1 to activate the Wnt/β-catenin signaling in cervical cancer. Bioengineered, 13(5), 12516-12531.

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Quercetin Modulates Macrophage Metabolism

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Quercetin and LPS were purchased from Sigma-Aldrich (St. Louis, MO, USA). The mouse macrophage-like cell line, RAW264.7 (CSTR:19375.09.3101MOUTCM13), was acquired from the National Collection of Authenticated Cell Cultures, the Chinese Academy of Sciences (Shanghai, China). Fetal bovine serum (FBS) and Dulbecco’s modified eagle’s medium (DMEM) high glucose were purchased from Gibcol Life Technology (Thermo Fisher, Waltham, MA, USA). An enhanced ATP Assay Kit was acquired from Beyotime^® Biotechnology (Shanghai, China). anti-PGC1 (Cat# ab191838), anti-glutathione peroxidase 4 (Cat# ab125066), anti-AMPK alpha 1 (Cat# ab32047) and anti-SIRT1 (Cat# ab110304) antibodies were purchased from Abcam Biotechnology (Cambridge, MA, USA). Phospho-AMPK (Thr172) (Cat# 2535s) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). The 3-4,5-dimethylthiazole-z-yl-3,5-diphenyltetrazolium bromide (MTT), trypsin, dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), LA Assay Kit, Micro Pyruvate (PA) Assay Kit and Mitochondrial Membrane Potential Assay Kit with JC-1 were purchased from Solarbio Science & Technology Co. Ltd. (Beijing, China), and stored at −20 °C. EX-527 and compound C were obtained from Med Chem Express (Monmouth Junction, NJ, USA).

Peng J., Yang Z., Li H., Hao B., Cui D., Shang R., Lv Y., Liu Y., Pu W., Zhang H., He J., Wang X, & Wang S. (2023). Quercetin Reprograms Immunometabolism of Macrophages via the SIRT1/PGC-1α Signaling Pathway to Ameliorate Lipopolysaccharide-Induced Oxidative Damage. International Journal of Molecular Sciences, 24(6), 5542.

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Investigating Neuroprotective Effects

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Cells were divided into four groups during EX527 treatment (control, OGD, OGD+EX527, OGD+EX527+RSV) and three groups during RSV treatment (control, OGD, OGD+RSV). Cells were pretreated with 25 μM EX527 (MedChemExpress, USA) for 3 hours before OGD, and post-treated with 20 μM RSV (Solarbio, China) immediately after OGD.

Shi L., Zhang J., Wang Y., Hao Q., Chen H, & Cheng X. (2020). Sirt1 Regulates Oxidative Stress in Oxygen-Glucose Deprived Hippocampal Neurons. Frontiers in Pediatrics, 8, 455.

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Modulation of C17.2 Neural Stem Cells

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Mouse C17.2 neural stem cells were maintained in minimum essential medium (Gibco-Invitrogen, Carlsbad, CA, United States) supplemented with 10% fetal bovine serum (Gibco), 1% penicillin/streptomycin (100 U/mL and 100 μg/mL, respectively; Life Technologies), and 1% minimum essential medium non-essential amino acids (Gibco) in a humidified environment at 37°C with 5% CO₂.
C17.2 cells were seeded in 6-well plates (1 × 10⁵ cells/well) and cultured before transfection. Plasmids pcDNA3.1(+)-CMV-Bhlhe40 and pcDNA3.1(+)-CMV-MCS were purchased from SyngenTech (Beijing, China). The cells were transfected with 1.5 μg plasmids or 100 pmol small interfering (si)RNAs in 2 mL of culture medium per well, using LipoFiter^TM liposomal transfection reagent (Hanbio Technology, China). After transfection for 48 h, the cells were harvested.
For Sirt1 activation or inhibition, RSV (a specific activator of Sirt1; 5 μM in DMSO) or EX527 (a specific inhibitor of Sirt1; 5 μM in DMSO; MedChemExpress) was added to the cell culture for 12 h.

Zhao L., Liu D., Ma W., Gu H., Wei X., Luo W, & Yuan Z. (2021). Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta. Frontiers in Cell and Developmental Biology, 9, 644346.

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