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Ab246832

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Ab246832 is a laboratory reagent. It is designed for use in scientific research applications.

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Lab products found in correlation

Ab64238, by Abcam (3 mentions) S7100, by Merck Group (3 mentions) Nanozoomer scanner, by Hamamatsu Photonics (3 mentions) Oct compound, by Sakura Finetek (3 mentions) Vectastatin elite abc hrp kit, by Vector Laboratories (1 mentions) Dab peroxidase substrate kit, by Vector Laboratories (1 mentions)

6 protocols using ab246832

1

Comprehensive Histological Characterization of Liver

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For histology, tissues were dissected and immediately fixed in 10% formalin overnight and processed for paraffin embedding and H&E staining. Paraffin-embedded liver sections were incubated with F4/80 antibody, and antibody was detected using a peroxidase-based method (Abcam, ab64238). Liver collagen was detected by Sirius Red staining (Abcam, ab246832) and apoptosis was detected by TUNEL staining (Millipore, S7100). For Oil Red O staining, liver tissue was frozen in OCT compound (Sakura Finetek, 4583), sectioned, and stained. Liver sections were imaged with a NanoZoomer Scanner (Hamamatsu) and quantification was done using NIH ImageJ.
Chen J., Wang R., Xiong F., Sun H., Kemper B., Li W, & Kemper J.K. (2024). Hammerhead-type FXR agonists induce an eRNA FincoR that ameliorates nonalcoholic steatohepatitis in mice. bioRxiv.
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2

Fibrin Architecture Visualization via Picrosirius Red

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Fibrin architecture was detected by PicroSirius red staining as previously reported [26 (link)]. Briefly, the PRF slides were deparaffinized, hydrated, treated with adequate picrosirius red solution (Abcam, ab246832), and incubated for 60 min. Finally, the stained slides were mounted on a resinous medium.
Nguyen-Thanh T., Nguyen-Tran B.S., Cruciani S., Nguyen-Thi T.D., Dang-Cong T, & Maioli M. (2023). Osteochondral Regeneration Ability of Uncultured Bone Marrow Mononuclear Cells and Platelet-Rich Fibrin Scaffold. Bioengineering, 10(6), 661.
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3

Liver Fibrosis Assessment in COVID-19

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Connective tissue stain (Sirius red) and immunohistochemistry (IHC) were performed using formalin-fixed, paraffin-embedded liver biopsy of four COVID-19 patients. For Sirius red staining, liver sections were dewaxed, rehydrated and stained for 2 minutes with hematoxylin, then 30 minutes with a picrosirius red solution (ab246832, Abcam). For IHC staining, antigen retrieval of dewaxed and rehydrated paraffin-embedded liver sections was performed using sodium citrate pH=6 for α-SMA and pepsin for CK19, respectively, followed by blocking with 10% goat serum for 30 minutes, and incubation with anti-α-SMA (Cell Signaling Technology, 19245, 1:400) and anti-CK19 (Sigma-Aldrich, MAB3238, 1:100) primary antibody overnight at 4 °C. After incubation with biotinylated secondary antibody for 1.5 hours, detection was performed with the Vectastatin Elite ABC-HRP kit (Vector Laboratories, SP-6100) with the DAB Peroxidase Substrate kit (Vector Laboratories, SK-4100), followed by counterstaining with haematoxylin.
Pita-Juarez Y., Karagkouni D., Kalavros N., Melms J.C., Niezen S., Delorey T.M., Essene A.L., Brook O.R., Pant D., Skelton-Badlani D., Naderi P., Huang P., Pan L., Hether T., Andrews T.S., Ziegler C.G., Reeves J., Myloserdnyy A., Chen R., Nam A., Phelan S., Liang Y., Amin A.D., Biermann J., Hibshoosh H., Veregge M., Kramer Z., Jacobs C., Yalcin Y., Phillips D., Slyper M., Subramanian A., Ashenberg O., Bloom-Ackermann Z., Tran V.M., Gomez J., Sturm A., Zhang S., Fleming S.J., Warren S., Beechem J., Hung D., Babadi M., Padera RF J.r., MacParland S.A., Bader G.D., Imad N., Solomon I.H., Miller E., Riedel S., Porter C.B., Villani A.C., Tsai L.T., Hide W., Szabo G., Hecht J., Rozenblatt-Rosen O., Shalek A.K., Izar B., Regev A., Popov Y., Jiang Z.G, & Vlachos I.S. (2022). A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients. bioRxiv.
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4

Comprehensive Histological Characterization of Liver

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For histology, tissues were dissected and immediately fixed in 10% formalin overnight and processed for paraffin embedding and H&E staining. Paraffin-embedded liver sections were incubated with F4/80 antibody, and antibody was detected using a peroxidase-based method (Abcam, ab64238). Liver collagen was detected by Sirius Red staining (Abcam, ab246832) and apoptosis was detected by TUNEL staining (Millipore, S7100). For Oil Red O staining, liver tissue was frozen in OCT compound (Sakura Finetek, 4583), sectioned, and stained. Liver sections were imaged with a NanoZoomer Scanner (Hamamatsu) and quantification was done using NIH ImageJ.
Chen J., Wang R., Xiong F., Sun H., Kemper B., Li W, & Kemper J. (2024). Hammerhead-type FXR agonists induce an enhancer RNA Fincor that ameliorates nonalcoholic steatohepatitis in mice. eLife, 13, RP91438.
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5

Comprehensive Liver Tissue Analysis

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Liver tissue was frozen in OCT compound (Sakura Finetek, 4583), sectioned, and stained with H&E or with Oil Red-O. Paraffin-embedded liver sections were incubated with F4/80 antibody, and antibody was detected using a peroxidase-based method (ab64238, Abcam). Liver collagen was detected by Sirius Red staining (Abcam, ab246832) and apoptosis was detected by TUNEL staining (Millipore, S7100). Liver sections were imaged with a NanoZoomer Scanner (Hamamatsu) and quantification was done using NIH ImageJ.
Sun H., Seok S., Jung H., Kemper B, & Kemper J.K. (2022). Obesity-induced miR-802 directly targets AMPK and promotes nonalcoholic steatohepatitis in mice. Molecular Metabolism, 66, 101603.
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6

Picrosirius Red Staining of PTX3-Treated HFL1 Cells

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Following the protocol mentioned in a previous study,27 HFL1 cells were seeded in 24‐well plates and treated with or without 100 ng/ml PTX3. Experimental cells were fixed with methanol at −20°C overnight. Cells were stained with Picrosirius Red Solution (ab246832, Abcam) for 1 h at room temperature and rinsed with an acetic acid solution. Quantification of the total number of nodule foci was determined visually under a microscope.
Chi J., Hsiao Y., Liang H., Huang T., Chen F., Chen C., Ko C., Cheng C, & Wang J. (2022). Blockade of the pentraxin 3/CD44 interaction attenuates lung injury‐induced fibrosis. Clinical and Translational Medicine, 12(11), e1099.
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