Ncm460

Colon cancer cell lines LOVO, HCT116, Caco-2, HT29, SW480, and SW1116 were obtained from American Type Culture Collection (ATCC) and subcultured and preserved by Shanghai Institute of Digestive Surgery; normal human colon mucosal epithelium cell NCM460 was kindly donated by Jingqing Zeng (Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; the donator bought NCM460 cell line from INCELL Corporation, LLC, San Antonio, TX, USA). LOVO was cultured in F-12K Nutrient Mixture Medium (Corning Cellgro®, MA, USA), HCT116 and HT29 in McCoy's 5A (Corning Cellgro®), SW480 and SW1116 in Leibovitz' L-15 (Corning Cellgro®), and NCM460 in DMEM (Corning Cellgro®). All culture medium above was supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA). Caco-2 was cultured in Minimum Essential Medium (Corning Cellgro®) with 20% FBS. Cells were placed in the incubator (Heracelles, Germany) at 37°C, in a humidified atmosphere with 5% CO₂.

The human CRC cell lines DLD1, HCT15, RKO, and SW620 were purchased from ATCC (Rockville, MD, USA), and the normal colonic epithelial cell line NCM460 was obtained from INCELL Corporation (San Antonio, TX, USA). The cells were cultured with DMEM (Thermo Fisher Scientific, San Jose, CA, USA) containing 10% FBS (ExCell Bio, Shanghai, China) in a 5% CO₂ cell incubator at 37 °C. NU2058 was purchased from Selleck Chemicals (Huston, TX, USA) and dissolved in DMSO.

Human colorectal cancer SW480, SW620, HCT116, HT29, RKO, LS174T, COLO-215, HCT8 and LoVo cell lines were purchased from American Type Culture Collection (Manassas, USA). Human normal intestinal epithelial cells NCM460 were purchased from INCELL Corporation LLC (San Antonio, USA). HCT116, HT29, RKO, LoVo, COLO-215, HCT8 and NCM460 cells are cultured in RPMI-1640. LS174T cells were cultured in DMEM. SW480 and SW620 were cultured in Leibovitz's L-15 medium. All cells were cultured at 37˚C containing 5% CO₂. All medium were supplemented with 10% fetal bovine serum (FBS, Gibco, USA).

HCT116, RKO, COLO205, LOVO, SW480, SW620, HT29 cells were purchased from the American Type Culture Collection (ATCC) and cultured according to the culture methods of ATCC. Colon normal immortalized epithelial cell line NCM460 was obtained from In Cell (San Antonio, TX) and cultured according to the method of manufacturer.